Recombinant Mouse IL-27 (NS0-expressed) Protein

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1 μg/lane of Recombinant Mouse IL-27 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 61 kDa.
Recombinant Mouse IL-27 (Catalog # 2799‑ML) demonstrates anti-viral activity in HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.6-6 ng/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse IL-27 (NS0-expressed) Protein Summary

Details of Functionality
Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Bender H. et al. (2009) Hepatology 50:585. The ED50 for this effect is 0.6-6 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse IL-27 protein
Mouse EBI3
(Tyr19-Pro228)
Accession # O35228
GGGSGGGSGGGSGGGS Mouse p28
(Phe29-Ser234)
Accession # Q8K3I6
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Tyr19
Protein/Peptide Type
Recombinant Proteins
Gene
Il27
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
48.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
60-65 kDa, reducing conditions
Publications
Read Publications using
2799-ML in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and CHAPS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-27 (NS0-expressed) Protein

  • IL-27 p28 subunit
  • IL-27 subunit alpha
  • IL27
  • IL-27
  • IL27A
  • IL-27-A
  • IL27-A
  • IL27p28
  • IL30
  • interleukin 27
  • interleukin 30
  • interleukin-27 subunit alpha
  • MGC71873
  • p28IL-27A

Background

IL-27 is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines (1). It is composed of EBI3 (EBV-induced gene 3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the cloned 28 kDa glycoprotein that is related to the p35 chain of IL-12 (2-4). The mouse EBI3 gene encodes a 228 amino acid (aa) precursor that contains an 18 aa signal peptide and a 210 aa mature protein (5). The mature region contains two potential N-linked glycosylation sites, two fibronectin type III domains, and two pairs of conserved cysteine residues that place the molecule in the type I cytokine receptor family (5). As with p40, (the EBI3 counterpart in IL-12), IL-27 EBI3 is reported to form homodimers (6). Mouse EBI3 is 61% and 66% aa identical to human and bovine EBI3. The mouse p28 gene encodes a 234 aa precursor that contains a 28 aa signal sequence and a 206 aa mature region (7). The mature region is characterized by the presence of one potential N-linked glycosylation site and four alpha -helices, placing it in the IL-6 family of helical cytokines. Mouse p28 is 74% aa identical to human p28. IL-27 is expressed by monocytes, endothelial cells and dendritic cells (8). IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX-1 (TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1 (7, 9, 10). IL-27 has both anti- and proinflammatory properties. As an anti-inflammatory, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity (3, 8). At the onset of infection, IL-27 induces an IL-12 receptor on naïve CD4+ T cells, making them susceptible to subsequent IL-12 activity (and possible Th1 development) (6). Notably, IL-12 family cytokines are both induced and inhibited by bacterial products. Microbes promote IL-27 secretion through TLR4, and also block IL-27 production via C5a induction (11).

  1. Boulay, J-L. et al. (2003) Immunity 19:159.
  2. Trinchieri, G. et al. (2003) Immunity 19:641.
  3. Murakami, M. et al. (2004) Growth Factors 22:75.
  4. Cordoba-Rodriguez, R. and D.M. Frucht (2003) Expert Opin. Biol. Ther. 3:715.
  5. Nomura, H. et al. (1997) GenBank Accession # O35228.
  6. Holscher, C. (2004) Med. Microbiol. Immunol. (Berl). 193:1.
  7. Pflanz, S. et al. (2002) Immunity 16:779.
  8. Villarino, A.V. et al. (2004) J. Immunol. 173:715.
  9. Pflanz, S. et al. (2004) J. Immunol. 172: 2225.
  10. Scheller, J. et al. (2005) Biochem. Biophys. Res. Commun. 326:724.
  11. Hawlish, H. et al. (2005) Immunity 22:415.

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2799-ML
Species: Mu
Applications: Bioactivity

Publications for IL-27 (2799-ML)(33)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: Bioassay, Cell Culture, Differentiation, In Vivo.


Filter By Application
Bioassay
(20)
Cell Culture
(1)
Differentiation
(1)
In Vivo
(11)
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Human
(2)
Mouse
(30)
Rat
(1)
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Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using 2799-ML Applications Species
M Matsuda, K Doi, T Tsutsumi, M Inaba, J Hamaguchi, T Terada, R Kawata, K Kitatani, T Nabe Adoptive transfer of type 1 regulatory T cells suppressed the development of airway hyperresponsiveness in ovalbumin-induced airway inflammation model mice J. Pharmacol. Sci., 2019;141(4):139-145. 2019 [PMID: 31744690] (Cell Culture, Mouse) Cell Culture Mouse
A Abdullah, M Maged, I Hairul-Isl, A Osama I, H Maha, A Manal, H Hamza Activation of aryl hydrocarbon receptor signaling by a novel agonist ameliorates autoimmune encephalomyelitis PLoS ONE, 2019;14(4):e0215981. 2019 [PMID: 31026283] (Bioassay, Mouse) Bioassay Mouse
QT Nguyen, E Jang, HT Le, S Kim, D Kim, N Dvorina, MA Aronica, WM Baldwin, K Asosingh, S Comhair, B Min IL-27 targets Foxp3+ Tregs to mediate antiinflammatory functions during experimental allergic airway inflammation JCI Insight, 2019;4(2):. 2019 [PMID: 30674714] (In Vivo, Mouse) In Vivo Mouse
R Aoki, A Aoki-Yoshi, C Suzuki, Y Takayama Indole-3-Pyruvic Acid, an Aryl Hydrocarbon Receptor Activator, Suppresses Experimental Colitis in Mice J. Immunol., 2018;0(0):. 2018 [PMID: 30429284] (Differentiation, Mouse) Differentiation Mouse
HA Ting, D de Almeida, AJ Rasky, CA Malinczak, IP Maillard, MA Schaller, NW Lukacs Notch ligand Delta-like 4 induces epigenetic regulation of Treg cell differentiation and function in viral infection Mucosal Immunol, 2018;0(0):. 2018 [PMID: 30038214] (Bioassay, Mouse) Bioassay Mouse
L Bouchareyc, EM Grössinger, M Kang, IE Adamopoulo ??TCR regulates production of interleukin-27 by neutrophils and attenuates inflammatory arthritis Sci Rep, 2018;8(1):7590. 2018 [PMID: 29765156] (Bioassay, Mouse) Bioassay Mouse
J Do, D Kim, S Kim, A Valentin-T, N Dvorina, E Jang, V Nagarajave, TM DeSilva, X Li, AH Ting, DAA Vignali, SA Stohlman, WM Baldwin, B Min Treg-specific IL-27R? deletion uncovers a key role for IL-27 in Treg function to control autoimmunity Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 28874534] (In Vivo, Mouse) In Vivo Mouse
WF Carson, KA Cavassani, EM Soares, S Hirai, NA Kittan, MA Schaller, MM Scola, A Joshi, A Matsukawa, DM Aronoff, CN Johnson, Y Dou, KA Gallagher, SL Kunkel The STAT4/MLL1 Epigenetic Axis Regulates the Antimicrobial Functions of Murine Macrophages J. Immunol., 2017;0(0):. 2017 [PMID: 28733487] (Bioassay, Mouse) Bioassay Mouse
IA Mufazalov, J Kuschmann, D Andruszews, J Masri, LA Gabriel, P Adams, S Reissig, N Hövelmeyer, A Waisman Balanced Bcl-3 expression in murine CD4(+) T cells is required for generation of encephalitogenic Th17 cells Eur. J. Immunol., 2017;0(0):. 2017 [PMID: 28598502] (Bioassay, Mouse) Bioassay Mouse
MT Stier, K Goleniewsk, JY Cephus, DC Newcomb, TP Sherrill, KL Boyd, MH Bloodworth, ML Moore, K Chen, JK Kolls, RS Peebles STAT1 Represses Cytokine-Producing Group 2 and Group 3 Innate Lymphoid Cells during Viral Infection J. Immunol., 2017;0(0):. 2017 [PMID: 28576981] (Bioassay, Mouse) Bioassay Mouse
Show All 33 Publications.

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Bioinformatics

Gene Symbol Il27
Uniprot