Recombinant Mouse IL-1ra/IL-1F3 Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse IL-1ra/IL-1F3 Protein Summary

Details of Functionality
Measured by its ability to inhibit IL-1 alpha -dependent proliferation in D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 15‑60 ng/mL in the presence of 50 pg/mL of recombinant human IL-1 alpha.
Source
E. coli-derived mouse IL-1ra/IL-1F3 protein
Arg8-Gln159, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
Il1rn
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
480-RM in the following applications:

Packaging, Storage & Formulations

Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Citric Acid, NaCl and EDTA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-1ra/IL-1F3 Protein

  • DIRA
  • ICIL-1ra
  • IL1F3
  • IL-1F3
  • IL1ra
  • IL-1ra
  • IL-1ra3
  • IL1RN
  • IL-1RN
  • interleukin 1 receptor antagonist
  • IRAP
  • MVCD4

Background

IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally-occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells. In mouse, IL-1 RII does not bind IL-1ra. The recombinant, non-glycosylated form of IL-1ra blocks binding of IL-1 to its receptor equally as well as the naturally-occurring, glycosylated form. The IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Pre-clinical and clinical studies were done to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.

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Publications for IL-1ra/IL-1F3/IL1RN (480-RM)(21)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, N/A.

We have publications tested in 3 applications: Bioassay, ELISA (Standard), In Vivo.


Filter By Application
Bioassay
(9)
ELISA (Standard)
(3)
In Vivo
(9)
All Applications
Filter By Species
Human
(1)
Mouse
(14)
Rat
(3)
N/A
(3)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using 480-RM Applications Species
HG Lee, JU Lee, DH Kim, S Lim, I Kang, JM Choi Pathogenic function of bystander-activated memory-like CD4+ T cells in autoimmune encephalomyelitis Nat Commun, 2019;10(1):709. 2019 [PMID: 30755603] (Bioassay, Mouse) Bioassay Mouse
HC Xiang, LX Lin, XF Hu, H Zhu, HP Li, RY Zhang, L Hu, WT Liu, YL Zhao, Y Shu, HL Pan, M Li AMPK activation attenuates inflammatory pain through inhibiting NF-?B activation and IL-1? expression J Neuroinflammation, 2019;16(1):34. 2019 [PMID: 30755236] (In Vivo, Mouse) In Vivo Mouse
Y Kang, H Zhang, Y Zhao, Y Wang, W Wang, Y He, W Zhang, W Zhang, X Zhu, Y Zhou, L Zhang, Z Ju, L Shi Telomere Dysfunction Disturbs Macrophage Mitochondrial Metabolism and the NLRP3 Inflammasome through the PGC-1?/TNFAIP3 Axis Cell Rep, 2018;22(13):3493-3506. 2018 [PMID: 29590618] (In Vivo, Mouse) In Vivo Mouse
MX Li, HL Zheng, Y Luo, JG He, W Wang, J Han, L Zhang, X Wang, L Ni, HY Zhou, ZL Hu, PF Wu, Y Jin, LH Long, H Zhang, G Hu, JG Chen, F Wang Gene deficiency and pharmacological inhibition of caspase-1 confers resilience to chronic social defeat stress via regulating the stability of surface AMPARs Mol. Psychiatry, 2017;0(0):. 2017 [PMID: 28416811] (In Vivo, Mouse) In Vivo Mouse
Interaction between interleukin-1? and type-1 cannabinoid receptor is involved in anxiety-like behavior in experimental autoimmune encephalomyelitis J Neuroinflammation, 2016;13(1):231. 2016 [PMID: 27589957] (In Vivo, Mouse) In Vivo Mouse
Reed M, Morris S, Owczarczyk A, Lukacs N Deficiency of autophagy protein Map1-LC3b mediates IL-17-dependent lung pathology during respiratory viral infection via ER stress-associated IL-1. Mucosal Immunol, 2015;8(5):1118-30. 2015 [PMID: 25669150] (In Vivo, Mouse) In Vivo Mouse
Shaw A, Pickup M, Chytil A, Aakre M, Owens P, Moses H, Novitskiy S TGFbeta signaling in myeloid cells regulates mammary carcinoma cell invasion through fibroblast interactions. PLoS ONE, 2015;10(1):e0117908. 2015 [PMID: 25629162] (Bioassay, Mouse) Bioassay Mouse
Cowardin C, Kuehne S, Buonomo E, Marie C, Minton N, Petri W Inflammasome activation contributes to interleukin-23 production in response to Clostridium difficile. MBio, 2015;6(1):. 2015 [PMID: 25626905] (Bioassay, Mouse) Bioassay Mouse
Khare S, Ratsimandresy R, de Almeida L, Cuda C, Rellick S, Misharin A, Wallin M, Gangopadhyay A, Forte E, Gottwein E, Perlman H, Reed J, Greaves D, Dorfleutner A, Stehlik C The PYRIN domain-only protein POP3 inhibits ALR inflammasomes and regulates responses to infection with DNA viruses. Nat Immunol, 2014;15(4):343-53. 2014 [PMID: 24531343] (Bioassay, Mouse) Bioassay Mouse
Zhao H, Zhao X, Cao X, Wu G Age-Dependent Neuroimmune Modulation of IGF-1R in the Traumatic Mice. Immun Ageing, 2012;9(1):12. 2012 [PMID: 22640633] (Bioassay, Mouse) Bioassay Mouse
Show All 21 Publications.

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Bioinformatics

Gene Symbol Il1rn
Uniprot