Recombinant Mouse IGF-II/IGF2 Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse IGF-II/IGF2 Protein, CF Summary

Details of Functionality
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 2-10 ng/mL.
Source
E. coli-derived mouse IGF-II/IGF2 protein
Ala25-Glu91
Accession #
N-terminal Sequence
Ala25
Protein/Peptide Type
Recombinant Proteins
Gene
Igf2
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
7.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
792-MG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IGF-II/IGF2 Protein, CF

  • C11orf43
  • chromosome 11 open reading frame 43
  • FLJ22066
  • FLJ44734
  • GRDF
  • IGF2
  • IGF-2
  • IGFII
  • IGF-II
  • insulin-like growth factor 2 (somatomedin A)
  • insulin-like growth factor II
  • insulin-like growth factor type 2
  • MSA
  • PEG2
  • PP9974
  • somatomedin-A

Background

IGF-II (Insulin-like growth factor II; also multiplication-stimulating polypeptide/MSP and somatomedin-A) is a secreted 8 kDa polypeptide that belongs to the insulin family of peptide growth factors (1, 2, 3). It is part of a complex system of growth and metabolic-regulating proteins that is particularly important during development. It has been associated with nervous system proliferation and differentiation, myelination, adrenal cortical proliferation, and skeletal growth and differentiation (4). In human, IGF-II is primarily synthesized by the liver, and circulates at high levels in both fetus and adult. In rodent, however, IGF-II levels drop after the perinatal period, an effect attributed to the lack of a key gene promoter (2, 5). This may indicate that postnatally, IGF-II has either a limited, or local effect only in rodent. For example, evidence suggests IGF-II may be the intermediary for SHH induction of VEGF attendant with local neovascularization (6). Rodent cells known to express IGF-II include astrocytes (7), hepatocytes (8), osteoblasts (9), embryonic striated muscle cells (10, 11) plus Kupffer cells and Ito cells (12). Mouse IGF-II is synthesized as a 180 amino acid (aa) preproprecursor (13). It contains a 24 aa signal sequence, a 67 aa mature region, and an 89 aa C-terminal prodomain that is alternatively referred to as the E-peptide. Mature IGF-II is 91% and 97% aa identical to human and rat IGF-II, respectively. Proper processing of IGF-II requires the chaperone activity of GRP94 (14). This generates an 8 kDa mature form, an 18 kDa, 156 aa proform, and a potential 11 kDa, 88 aa “Big” form (aa 25-112).  This 11 kDa ”Big” form would be equivalent to human 15-16 kDa IGF-II, with the 5 kDa difference attributable to the presence of O-linked glycosylation (15).  There is an additional 34 aa proteolytic fragment that is termed preptin and contains aa 93-126 of the preproprecursor.  This is distinct from IGF-II, is secreted by pancreatic b cells, and facilitates insulin secretion (16, 17). IGF-II has multiple binding partners.  It binds to IGF-IR, the Insulin receptor (IR)-type A and IGF-IR:IR-A hybrids, the type 2 IGF receptor (IGF-2R), and IGF binding proteins 1-6 (18, 19).  The first three receptors initiate downstream signaling events, the IGF-2R sequesters local IGF-II, and the six IGFBPs regulate IGF-II activity in various tissues.

  1. LeRoith, D. & C.T. Roberts Jr. (2003) Cancer Lett. 195:127.
  2. Werner, H. & D. LeRoith (2000) Cell. Mol. Life Sci. 57:932.
  3. Pavelic, J. et al. (2007) Indian J. Med. Res. 125:511.
  4. Varela-Nieto, I. et al. (2007) Curr. Pharm. Des. 13:687.
  5. Rotwein, P. & L.J. Hall (1990) DNA Cell Biol. 10:725.
  6. Chao, W. & P. A. D-Amore (2008) Cytokine Growth Factor Rev. 19:111.
  7. Rotwein, P. et al. (1988) Proc. Natl. Acad. Sci. USA 85:265.
  8. Goya, L. et al. (1999) J. Biol. Chem. 274:24633.
  9. McCarthy, T.L. et al. (1992) Endocrinology 130:1303.
  10. Zindy, F. et al. (1992) J. Hepatol. 14:30.
  11. Holthuizen, P.E. et al. (1993) Regul. Pept. 48:77.
  12. Merrick, D. et al. (2007) BMC Dev. Biol. 7:65.
  13. Stempien, M.M. et al. (1986) DNA 5:357.
  14. Ostrovsky, O. et al. (2009) Mol. Biol. Cell 20:1855.
  15. Daughaday, W.H. et al. (1993) Proc. Natl. Acad. Sci. USA 90:5823.
  16. Buchanan, C.M. et al. (2001) Biochem. J. 360:431.
  17. Cornish, J. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292:E117.
  18. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.
  19. Belfiore, A. (2007) Curr. Pharm. Des. 13:671.

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Publications for IGF-II/IGF2 (792-MG)(18)

We have publications tested in 3 confirmed species: Mouse, Rat, N/A.

We have publications tested in 5 applications: Bioassay, ELISA (Capture), ELISA (Standard), In Vivo, Western Blot.


Filter By Application
Bioassay
(10)
ELISA (Capture)
(1)
ELISA (Standard)
(1)
In Vivo
(5)
Western Blot
(1)
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Filter By Species
Mouse
(16)
Rat
(1)
N/A
(1)
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Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using 792-MG Applications Species
KL Lee, JF Aitken, HL Hsu, GM Williams, MA Brimble, GJS Cooper Glucoregulatory activity of vesiculin in insulin sensitive and resistant mice Peptides, 2019;116(0):1-7. 2019 [PMID: 31018156] (In Vivo, Mouse) In Vivo Mouse
C De Giovann, P Nanni, L Landuzzi, ML Ianzano, G Nicoletti, S Croci, A Palladini, PL Lollini Immune targeting of autocrine IGF2 hampers rhabdomyosarcoma growth and metastasis BMC Cancer, 2019;19(1):126. 2019 [PMID: 30732578] (ELISA (Capture), Mouse) ELISA (Capture) Mouse
P Periasamy, V Tran, HC O'Neill Identification of genes which regulate stroma-dependent in vitro hematopoiesis PLoS ONE, 2018;13(10):e0205583. 2018 [PMID: 30308055] (Bioassay, Mouse) Bioassay Mouse
MJ Wang, F Chen, QG Liu, CC Liu, H Yao, B Yu, HB Zhang, HX Yan, Y Ye, T Chen, KJ Wangenstee, X Wang, YP Hu, ZY He Insulin-like growth factor 2 is a key mitogen driving liver repopulation in mice Cell Death Dis, 2018;9(2):26. 2018 [PMID: 29348399] (Bioassay, Mouse) Bioassay Mouse
AB Steinmetz, SA Stern, AS Kohtz, G Descalzi, CM Alberini Insulin-Like Growth Factor II Targets the mTOR Pathway to Reverse Autism-Like Phenotypes in Mice J. Neurosci., 2018;38(4):1015-1029. 2018 [PMID: 29217683] (In Vivo, Mouse) In Vivo Mouse
M Pardo, Y Cheng, D Velmeshev, M Magistri, H Eldar-Fink, A Martinez, MA Faghihi, RS Jope, E Beurel Intranasal siRNA administration reveals IGF2 deficiency contributes to impaired cognition in Fragile X syndrome mice JCI Insight, 2017;2(6):e91782. 2017 [PMID: 28352664] (In Vivo, Mouse) In Vivo Mouse
Nakatsuka R, Iwaki R, Matsuoka Y, Sumide K, Kawamura H, Fujioka T, Sasaki Y, Uemura Y, Asano H, Kwon A, Sonoda Y Identification and Characterization of Lineage(-)CD45(-)Sca-1(+) VSEL Phenotypic Cells Residing in Adult Mouse Bone Tissue. Stem Cells Dev, 2015;25(1):27-42. 2015 [PMID: 26595762] (Bioassay, Mouse) Bioassay Mouse
Casellas A, Mallol C, Salavert A, Jimenez V, Garcia M, Agudo J, Obach M, Haurigot V, Vila L, Molas M, Lage R, Morro M, Casana E, Ruberte J, Bosch F Insulin-like Growth Factor 2 Overexpression Induces beta-Cell Dysfunction and Increases Beta-cell Susceptibility to Damage. J Biol Chem, 2015;290(27):16772-85. 2015 [PMID: 25971976] (Bioassay, Mouse) Bioassay Mouse
Ikemoto-Uezumi M, Uezumi A, Tsuchida K, Fukada S, Yamamoto H, Yamamoto N, Shiomi K, Hashimoto N Pro-Insulin-Like Growth Factor-II Ameliorates Age-Related Inefficient Regenerative Response by Orchestrating Self-Reinforcement Mechanism of Muscle Regeneration. Stem Cells, 2015;33(8):2456-68. 2015 [PMID: 25917344] (Western Blot, Mouse) Western Blot Mouse
Shi Y, Chen J, Karner C, Long F Hedgehog signaling activates a positive feedback mechanism involving insulin-like growth factors to induce osteoblast differentiation. Proc Natl Acad Sci U S A, 2015;112(15):4678-83. 2015 [PMID: 25825734] (Bioassay, Mouse) Bioassay Mouse
Show All 18 Publications.

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Bioinformatics

Gene Symbol Igf2
Uniprot