Recombinant Mouse IFNAA Protein, CF

Recombinant Mouse IFNAA (Catalog # 10150-IF) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is <15 pg/mL.

2 μg/lane of Recombinant Mouse IFNAA (Catalog # 10150-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 16-21 kDa.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse IFNAA Protein, CF Summary

• Details of Functionality: Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED₅₀ for this effect is <15 pg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse IFNAA protein
  Cys24-Glu190
• Accession #: Q61718
• N-terminal Sequence: Cys24
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 19.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 16-21 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IFNAA Protein, CF

• Gm12597
• IFNA15
• IFNAA

Background

Interferon-alpha A (IFNAA), also referred to as interferon-alpha 15 (IFNA15), is one of 14 subtypes within the IFN-alpha family (1). The members of the IFN-alpha family, also known as alpha leukocyte interferons, encompass a group of distinct but closely related proteins which share approximately 80% amino acid (aa) sequence identity and have a similar globular structure composed of five alpha-helices (1-3). IFN-alpha family members signal through a common set of cell surface receptor complex composed of IFNAR2 and IFNAR1 subunits (2). The mature extracellular domain (ECD) of mouse IFNAA is 167 aa and shares 93% and 92% aa sequence identity with mouse and rat, respectively. Most of the members of the murine IFN-alpha family are N-glycosylated, however IFNAA is one of 4 members that lacks a N-glycosylation site (3). IFNAA is one of the many interferon alpha subtypes belonging to the Type I leukocyte IFN family. The Type I IFN family has antiviral, anti-proliferative and natural killer cell activities.

Publications for IFN-alpha 15/IFNA15 (10150-IF)(1)

Publication using 10150-IF

• Lu, J;Hu, Z;Jiang, H;Wen, Z;Li, H;Li, J;Zeng, K;Xie, Y;Chen, H;Su, XZ;Cai, C;Yu, X; Dual nature of type I interferon responses and feedback regulations by SOCS1 dictate malaria mortality Journal of advanced research 2024-08-22 [PMID: 39181199] (In vivo assay, Mouse, Transgenic Mouse)

Bioinformatics

• Uniprot:
  • Q61718()