Recombinant Mouse IFN-kappa Protein

Recombinant Mouse IFN-kappa (Catalog # 8437-MK) exhibits anti-viral activity in L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.2-1.2 ng/mL.

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse IFN-kappa Protein Summary

• Details of Functionality: Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED₅₀ for this effect is 0.2-1.2 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse IFN-kappa protein
  Leu22-Lys199
• Accession #: Q7TSL0
• N-terminal Sequence: Leu22
• Protein/Peptide Type: Recombinant Proteins
• Gene: IFNK
• Purity: >95%, by SDS-PAGE with silver staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 19-22 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Sodium Acetate, NaCl and EDTA with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE with silver staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IFN-kappa Protein

• IFNK
• IFNkappa
• IFN-kappa

Background

Interferon (IFN)-kappa is a member of the type I IFN family, which also includes IFN-alpha , -beta , -epsilon , and -omega . Mouse IFN-kappa is expressed at low levels in peritoneal macrophages and its expression is up-regulated by double-stranded (ds) RNA and IFN-gamma (1). Mice over-expressing IFN-kappa in pancreatic beta  cells developed type I diabetes, similar to what has been reported for mice over-expressing IFN-alpha , -beta , and -gamma (1-4). Mouse IFN-kappa shares 68% and 30% amino acid sequence identity with rat and human IFN-kappa , respectively. Human IFN-kappa has been detected in keratinocytes, monocytes, and monocyte-derived dendritic cells and is reported to have contact-dependent antiviral activity (5-7). Human papillomavirus (HPV) 16 oncogene expression, which is necessary for the development of cervical cancer, has been shown to down-regulate human IFN-kappa expression (8-11).

1. Vassileva, G. et al. (2003) J. Immunol. 170:5748.
2. Stewart, T.A. et al. (1993) Science 260:1942.
3. Sarvetnick, N. et al. (1988) Cell 52:773.
4. Pelegrin, M. et al. (1998) J. Biol. Chem. 273:12332.
5. LaFleur, D.W. et al. (2001) J. Biol. Chem. 276:39765.
6. Nardelli, B. et al. (2002) J. Immunol. 169:4822.
7. Buontempo, P.J. et al. (2006) J. Interferon Cytokine Res. 26:40.
8. Rincon-Orozco, B. et al. (2009) Cancer Res. 69:8718.
9. DeCarlo, C.A. et al. (2010) Lab. Invest. 90:1482.
10. Reiser, J. et al. (2011) J. Virol. 85:11372.
11. Sunthamala, N. et al. (2014) PLoS One 9:e91473.

Publications for IFN-kappa (8437-MK)(2)

Publications using 8437-MK

• Yang, N;Sun, Y;Han, B;Deng, N;Li, G;Han, Q;Wang, Y;Cai, H;Liu, F;Cao, B;Deng, W;Bao, H;Kong, S;Lu, J;Wang, H; Trophoblastic signals facilitate endometrial interferon response and lipid metabolism, ensuring normal decidualization Cell reports 2024-05-18 [PMID: 38762885] (In vivo assay, Mouse)
• SJ Wolf, CO Audu, A Joshi, AD denDekker, WJ Melvin, FM Davis, X Xing, R Wasikowski, L Tsoi, S Kunkel, JE Gudjonsson, MX O'Riordan, JM Kahlenberg, KA Gallagher Interferon kappa (IFNk) is critical for normal wound repair and is decreased in diabetic wounds JCI Insight, 2022-05-09;0(0):. 2022-05-09 [PMID: 35358091] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: IFNK
• Uniprot:
  • Q7TSL0()