Recombinant Mouse IFN-beta Protein

Recombinant Mouse IFN-beta (Catalog # 8234-MB) suppresses viral activity using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus.The ED50 for this effect is 1.00-6.00 pg/mL.

1 μg/lane of Recombinant Mouse IFN-beta (Catalog # 8234-MB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 32.7 and 32.9 kDa, respectively.

• Applications: Bioactivity

Recombinant Mouse IFN-beta Protein Summary

• Details of Functionality: Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED₅₀ for this effect is 1.00-6.00 pg/mL.
• Source: Human embryonic kidney cell, HEK293-derived mouse IFN-beta protein
  Ile22-Asn182
• Accession #: P01575
• N-terminal Sequence: Ile22
• Structure / Form: Monomer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Ifnb1
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 30-38 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and Tween® 80 with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IFN-beta Protein

• Fibroblast interferon
• IFB
• IFBIFNB
• IFF
• IFNB
• IFNB1
• IFNbeta
• IFN-beta
• interferon beta
• interferon, beta 1, fibroblast
• MGC96956

Background

Interferon beta (IFN-beta ), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules (1). Mature mouse IFN-beta shares 75% and 47% amino acid sequence identity with the rat and human proteins, respectively. Fibroblasts are the major producers of IFN-beta , but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogens (2). It is transcriptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3, 4). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) (5). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS (6-10). IFN-beta can additionally induce the expression of the anti-inflammatory cytokine IL-10 (11).

1. González-Navajas, J.M. et al. (2012) Nat. Rev. Immunol. 12:125.
2. Reder, A.T. and X. Feng (2013) Front. Immunol. 4:281.
3. Schafer, S.L. et al. (1998) J. Biol. Chem. 273:2714.
4. Häcker, H. et al. (2006) Nature 439:204.
5. Teige, I. et al. (2003) J. Immunol. 170:4776.
6. Shinohara, M.L. et al. (2008) Immunity 29:68.
7. Guo, B. et al. (2008) J. Clin. Invest. 118:1680.
8. Ramgolam, V.S. and S. Markovic-Plese (2010) Endocr. Metab. Immune Disord. Drug Targets 10:161.
9. Martín-Saavedra, F.M. et al. (2008) Mol. Immunol. 45:4008.
10. Inoue, M. and M.L. Shinohara (2013) Immunology 139:11.
11. Wang, H. et al. (2011) J. Immunol 186:675.

Publications for IFN-beta (8234-MB)(36)

Publications using 8234-MB

• Calcines-Rodríguez, L;Noyes-Martel, N;Becker, JL;Nguyen, SK;Osabutey, MA;Trojanczyk, LA;Majewska, AK;O'Banion, MK; Microglial interferon signaling and A? plaque pathology are enhanced in female 5xFAD Alzheimer's disease mice, independent of estrous cycle stage Journal of neuroinflammation 2025-12-27 [PMID: 41455993] (In vivo assay, Mouse)
• Shenoy, G;Sahu, A;Kuhn, M;Khristov, V;Heebner, M;Wilding, H;Clegg, T;Bhanja, D;Wade, Q;Liermann, LJ;Wang, D;Smith, N;Remite-Berthet, G;Khunsriraksakul, C;Palsa, K;Slagle-Webb, B;Mansouri, A;Zacharia, BE;Proctor, EA;Connor, JR; Analysis of transition metal content in glioblastoma reveals association between iron and survival Translational oncology 2025-03-30 [PMID: 40163909] (Cell Culture, Mouse)
• Kobayashi, M;Kobayashi, N;Deguchi, K;Omori, S;Nagai, M;Fukui, R;Song, I;Fukuda, S;Miyake, K;Ichinohe, T; TNF-? exacerbates SARS-CoV-2 infection by stimulating CXCL1 production from macrophages PLoS pathogens 2024-12-01 [PMID: 39652608] (In vivo assay, Mouse)
• Rajwani, J;Vishnevskiy, D;Turk, M;Naumenko, V;Gafuik, C;Kim, DS;Mah, LK;Snelling, S;Gonzales, GA;Xue, J;Chanda, A;Potts, KG;Todesco, HM;Lau, KCK;Hildebrand, KM;Chan, JA;Liao, S;Monument, MJ;Hyrcza, M;Bose, P;Jenne, CN;Canton, J;Zemp, FJ;Mahoney, DJ; VSV?M51 drives CD8+ T cell-mediated tumour regression through infection of both cancer and non-cancer cells Nature communications 2024-11-15 [PMID: 39548070] (Bioassay, Mouse)
• Lu, J;Hu, Z;Jiang, H;Wen, Z;Li, H;Li, J;Zeng, K;Xie, Y;Chen, H;Su, XZ;Cai, C;Yu, X; Dual nature of type I interferon responses and feedback regulations by SOCS1 dictate malaria mortality Journal of advanced research 2024-08-22 [PMID: 39181199] (In vivo assay, Mouse, Transgenic Mouse)
• Shuster, M;Lyu, Z;Augenstreich, J;Mathur, S;Ganesh, A;Ling, J;Briken, V; Salmonella Typhimurium infection inhibits macrophage IFN? signaling in a TLR4-dependent manner bioRxiv : the preprint server for biology 2024-03-05 [PMID: 38496427] (Bioassay, Mouse)
• Hardaker, EL;Sanseviero, E;Karmokar, A;Taylor, D;Milo, M;Michaloglou, C;Hughes, A;Mai, M;King, M;Solanki, A;Magiera, L;Miragaia, R;Kar, G;Standifer, N;Surace, M;Gill, S;Peter, A;Talbot, S;Tohumeken, S;Fryer, H;Mostafa, A;Mulgrew, K;Lam, C;Hoffmann, S;Sutton, D;Carnevalli, L;Calero-Nieto, FJ;Jones, GN;Pierce, AJ;Wilson, Z;Campbell, D;Nyoni, L;Martins, CP;Baker, T;Serrano de Almeida, G;Ramlaoui, Z;Bidar, A;Phillips, B;Boland, J;Iyer, S;Barrett, JC;Loembé, AB;Fuchs, SY;Duvvuri, U;Lou, PJ;Nance, MA;Gomez Roca, CA;Cadogan, E;Critichlow, SE;Fawell, S;Cobbold, M;Dean, E;Valge-Archer, V;Lau, A;Gabrilovich, DI;Barry, ST; The ATR inhibitor ceralasertib potentiates cancer checkpoint immunotherapy by regulating the tumor microenvironment Nature communications 2024-02-24 [PMID: 38402224] (Bioassay, Mouse)
• Zhang, H;Xue, K;Li, W;Yang, X;Gou, Y;Su, X;Qian, F;Sun, L; Cullin5 drives experimental asthma exacerbations by modulating alveolar macrophage antiviral immunity Nature communications 2024-01-04 [PMID: 38177117] (In vivo assay, Mouse, Transgenic Mouse)
• Byun, H;Singh, GB;Xu, WK;Das, P;Reyes, A;Battenhouse, A;Wylie, DC;Lozano, MM;Dudley, JP; Apobec-Mediated Retroviral Hypermutation In Vivo is Dependent on Mouse Strain bioRxiv : the preprint server for biology 2023-11-02 [PMID: 37961113] (Bioassay, Mouse)
• Xia, L;Komissarova, A;Jacover, A;Shovman, Y;Arcila-Barrera, S;Tornovsky-Babeay, S;Jaya Prakashan, MM;Nasereddin, A;Plaschkes, I;Nevo, Y;Shiff, I;Yosefov-Levi, O;Izhiman, T;Medvedev, E;Eilon, E;Wilensky, A;Yona, S;Parnas, O; Systematic identification of gene combinations to target in innate immune cells to enhance T cell activation Nature communications 2023-10-09 [PMID: 37813864] (Bioassay, Mouse, Transgenic Mouse)
• Li, Y;Xue, M;Deng, X;Dong, L;Nguyen, LXT;Ren, L;Han, L;Li, C;Xue, J;Zhao, Z;Li, W;Qing, Y;Shen, C;Tan, B;Chen, Z;Leung, K;Wang, K;Swaminathan, S;Li, L;Wunderlich, M;Mulloy, JC;Li, X;Chen, H;Zhang, B;Horne, D;Rosen, ST;Marcucci, G;Xu, M;Li, Z;Wei, M;Tian, J;Shen, B;Su, R;Chen, J; TET2-mediated mRNA demethylation regulates leukemia stem cell homing and self-renewal Cell stem cell 2023-08-03 [PMID: 37541212]
• P Dhillon, KA Mulholland, H Hu, J Park, X Sheng, A Abedini, H Liu, A Vassalotti, J Wu, K Susztak Increased levels of endogenous retroviruses trigger fibroinflammation and play a role in kidney disease development Nature Communications, 2023-02-02;14(1):559. 2023-02-02 [PMID: 36732547] (Bioassay, Mouse)
• C Manet, Z Mansuroglu, L Conquet, V Bortolin, T Comptdaer, H Segrt, M Bourdon, R Menidjel, N Stadler, G Tian, F Herit, F Niedergang, S Souès, L Buée, MC Galas, X Montagutel, E Bonnefoy Zika virus infection of mature neurons from immunocompetent mice generates a disease-associated microglia and a tauopathy-like phenotype in link with a delayed interferon beta response Journal of Neuroinflammation, 2022-12-20;19(1):307. 2022-12-20 [PMID: 36539803] (Cell Culture, Mouse)
• D Kwart, J He, S Srivatsan, C Lett, J Golubov, EM Oswald, P Poon, X Ye, J Waite, AG Zaretsky, S Haxhinasto, E Au-Yeung, NT Gupta, J Chiu, C Adler, S Cherravuru, E Malahias, N Negron, K Lanza, A Coppola, M Ni, H Song, Y Wei, GS Atwal, L Macdonald, NS Oristian, W Poueymirou, V Jankovic, M Fury, I Lowy, AJ Murphy, MA Sleeman, B Wang, D Skokos Cancer cell-derived type I interferons instruct tumor monocyte polarization Cell Reports, 2022-12-06;41(10):111769. 2022-12-06 [PMID: 36476866] (Bioassay, Mouse)
• N Zoellner, N Coesfeld, FH De Vos, J Denter, HC Xu, E Zimmer, B Knebel, H Al-Hasani, S Mossner, PA Lang, DM Floss, J Scheller Synthetic mimetics assigned a major role to IFNAR2 in type I interferon signaling Frontiers in Microbiology, 2022-09-02;13(0):947169. 2022-09-02 [PMID: 36118237] (Bioassay, Mouse)
• Zhouyangfan Peng, Mingwu Duan, Yiting Tang, Jianfeng Wu, Kai Zhao, Yanjun Zhong et al. Impaired interferon-gamma signaling promotes the development of silicosis iScience 2022-07-15 [PMID: 35800765] (Bioassay, Transgenic Mouse)
• Alexis R. Gibson, Adam Sateriale, Jennifer E. Dumaine, Julie B. Engiles, Ryan D. Pardy, Jodi A. Gullicksrud et al. A genetic screen identifies a protective type III interferon response to Cryptosporidium that requires TLR3 dependent recognition PLOS Pathogens 2022-05-18 [PMID: 35584177] (In vivo assay, Mouse)
• LC Dorman, PT Nguyen, CC Escoubas, ID Vainchtein, Y Xiao, PV Lidsky, H Nakajo, NJ Silva, C Lagares-Li, EY Wang, SE Taloma, B Cuevas, H Nakao-Inou, BM Rivera, B Schwer, C Condello, R Andino, TJ Nowakowski, AV Molofsky A type I interferon response defines a conserved microglial state required for effective neuronal phagocytosis bioRxiv : the preprint server for biology, 2023-03-20;0(0):. 2023-03-20 [PMID: 35233577] (Mouse)
• A Fedoriw, L Shi, S O'Brien, KN Smitheman, Y Wang, J Hou, C Sherk, S Rajapurkar, J Laraio, LJ Williams, C Xu, G Han, Q Feng, MT Bedford, L Wang, O Barbash, RG Kruger, P Hwu, HP Mohammad, W Peng Inhibiting Type I arginine methyltransferase activity promotes the T cell mediated antitumor immune response Cancer Immunology Research, 2022-04-01;0(0):. 2022-04-01 [PMID: 35181787] (Bioassay, Mouse)
• L Yang, S Chen, Q Zhao, C Pan, L Peng, Y Han, L Li, J Ruan, J Xia, H Yang, F Xu, G Cheng Histone deacetylase 3 contributes to the antiviral innate immunity of macrophages by interacting with FOXK1 to regulate STAT1/2 transcription Cell Reports, 2022-01-25;38(4):110302. 2022-01-25 [PMID: 35081346] (Bioassay, Mouse)
• NL Ka, GY Lim, S Hwang, SS Kim, MO Lee IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer Cell Reports, 2021-12-21;37(12):110138. 2021-12-21 [PMID: 34936865] (Bioassay, Mouse)
• JMJ Tan, ME Garner, JM Regeimbal, CJ Greene, JDR Márquez, DA Ammendolia, ARR McCluggage, T Li, KJ Wu, M Cemma, PP Ostrowski, B Raught, MS Diamond, S Grinstein, RM Yates, DE Higgins, JH Brumell Listeria exploits IFITM3 to suppress antibacterial activity in phagocytes Nature Communications, 2021-08-17;12(1):4999. 2021-08-17 [PMID: 34404769] (Bioassay, Mouse)
• LM Sanmarco, MA Wheeler, C Gutiérrez-, CM Polonio, M Linnerbaue, FA Pinho-Ribe, Z Li, F Giovannoni, KV Batterman, G Scalisi, SEJ Zandee, ES Heck, M Alsuwailm, DL Rosene, B Becher, IM Chiu, A Prat, FJ Quintana Gut-licensed IFN&amp;gamma+ NK cells drive LAMP1+TRAIL+ anti-inflammatory astrocytes Nature, 2021-01-06;0(0):. 2021-01-06 [PMID: 33408417] (Bioassay, Mouse)
• X Guo, P Ma, Y Li, Y Yang, C Wang, T Xu, H Wang, C Li, B Mao, X Qi RNF220 mediates K63-linked polyubiquitination of STAT1 and promotes host defense Cell Death Differ., 2020-08-19;0(0):. 2020-08-19 [PMID: 32814877] (Bioassay, Human, Mouse)
• D Giraldo, DR Wilcox, R Longnecker The Innate Immune Response to Herpes Simplex Virus 1 Infection Is Dampened in the Newborn Brain and Can Be Modulated by Exogenous Interferon Beta To Improve Survival MBio, 2020-05-26;11(3):. 2020-05-26 [PMID: 32457247] (In Vivo, Mouse)
• S Hosseini, K Michaelsen, G Grigoryan, C Chhatbar, U Kalinke, M Korte Type I Interferon Receptor Signaling in Astrocytes Regulates Hippocampal Synaptic Plasticity and Cognitive Function of the Healthy CNS Cell Rep, 2020-05-19;31(7):107666. 2020-05-19 [PMID: 32433975] (Mouse)
• Ziegler C, Allon S, Nyquist S, Mbano I, Miao V, Tzouanas C, Cao Y, Yousif A, Bals J, Hauser B, Feldman J, Muus C, Wadsworth M, Kazer S, Hughes T, Doran B, Gatter G, Vukovic M, Taliaferro F, Mead B, Guo Z, Wang J, Gras D, Plaisant M, Ansari M, Angelidis I, Adler H, Sucre J, Taylor C, Lin B, Waghray A, Mitsialis V, Dwyer D, Buchheit K, Boyce J, Barrett N, Laidlaw T, Carroll S, Colonna L, Tkachev V, Peterson C, Yu A, Zheng H, Gideon H, Winchell C, Lin P, Bingle C, Snapper S, Kropski J, Theis F, Schiller H, Zaragosi L, Barbry P, Leslie A, Kiem H, Flynn J, Fortune S, Berger B, Finberg R, Kean L, Garber M, Schmidt A, Lingwood D, Shalek A, Ordovas-Montanes J SARS-CoV-2 Receptor ACE2 Is an Interferon-Stimulated Gene in Human Airway Epithelial Cells and Is Detected in Specific Cell Subsets across Tissues. Cell, 2020-04-27;181(5):1016-1035.e19. 2020-04-27 [PMID: 32413319] (Bioassay, Mouse)
• N Muthalagu, T Monteverde, X Raffo-Irao, R Wiesheu, D Whyte, A Hedley, S Laing, B Kruspig, R Upstill-Go, R Shaw, S Neidler, C Rink, SA Karim, K Gyuraszova, C Nixon, W Clark, AV Biankin, LM Carlin, SB Coffelt, OJ Sansom, JP Morton, DJ Murphy Repression of the Type I Interferon pathway underlies MYC &amp; KRAS-dependent evasion of NK &amp; B cells in Pancreatic Ductal Adenocarcinoma Cancer Discov, 2020-03-21;0(0):. 2020-03-21 [PMID: 32200350] (Bioassay, Mouse)
• Samira Mansouri, Divya S. Katikaneni, Himanshu Gogoi, Mauricio Pipkin, Tiago N. Machuca, Amir M. Emtiazjoo et al. Lung IFNAR1hi TNFR2+ cDC2 promotes lung regulatory T cells induction and maintains lung mucosal tolerance at steady state Mucosal Immunology 2020-07-01 [PMID: 31959883] (In vivo assay, Transgenic Mouse)
• SW Wong, BW Huang, X Hu, E Ho Kim, JP Kolb, RJ Padilla, P Xue, L Wang, TH Oguin, PA Miguez, HC Tseng, CC Ko, J Martinez Global deletion of Optineurin results in altered type I IFN signaling and abnormal bone remodeling in a model of Paget&#039;s disease Cell Death Differ., 2019-05-10;0(0):. 2019-05-10 [PMID: 31076632] (Bioassay, Mouse)
• AM Giebel, S Hu, K Rajaram, R Finethy, E Toh, JA Brothwell, SG Morrison, RJ Suchland, BD Stein, J Coers, RP Morrison, DE Nelson Genetic Screen in Chlamydia muridarum Reveals Role for an Interferon-Induced Host Cell Death Program in Antimicrobial Inclusion Rupture MBio, 2019-04-09;10(2):. 2019-04-09 [PMID: 30967464] (Bioassay, Mouse)
• A Singanayag, N Glanville, JL Girkin, YM Ching, A Marcellini, JD Porter, M Toussaint, RP Walton, LJ Finney, J Aniscenko, J Zhu, MB Trujillo-T, MA Calderazzo, C Grainge, SL Loo, PC Veerati, PS Pathinayak, KS Nichol, AT Reid, PL James, R Solari, PAB Wark, DA Knight, MF Moffatt, WO Cookson, MR Edwards, P Mallia, NW Bartlett, SL Johnston Corticosteroid suppression of antiviral immunity increases bacterial loads and mucus production in COPD exacerbations Nat Commun, 2018-06-08;9(1):2229. 2018-06-08 [PMID: 29884817] (In Vivo, Mouse)
• P Mukherjee, G Hough, A Chattopadh, M Navab, HR Fogelman, D Meriwether, K Williams, S Bensinger, T Moller, KF Faull, AJ Lusis, L Iruela-Ari, KI Bostrom, P Tontonoz, ST Reddy, AM Fogelman Transgenic Tomatoes Expressing the 6F Peptide and Ezetimibe Prevent Diet-induced Increases of Interferon-? and Cholesterol 25-hydroxylase in Jejunum J. Lipid Res., 2017-06-07;0(0):. 2017-06-07 [PMID: 28592401] (ELISA (Standard))
• A Hunger, RF Medrano, DB Zanatta, PR Del Valle, CA Merkel, TA Salles, DG Ferrari, TK Furuya, SO Bustos, R de Freitas, E Costanzi-S, BE Strauss Reestablishment of p53/Arf and interferon-? pathways mediated by a novel adenoviral vector potentiates antiviral response and immunogenic cell death Cell Death Discov, 2017-03-20;3(0):17017. 2017-03-20 [PMID: 28386458] (Bioassay, Mouse)
• Y Xiao, Q Zou, X Xie, T Liu, HS Li, Z Jie, J Jin, H Hu, G Manyam, L Zhang, X Cheng, H Wang, I Marie, DE Levy, SS Watowich, SC Sun The kinase TBK1 functions in dendritic cells to regulate T cell homeostasis, autoimmunity, and antitumor immunity J. Exp. Med., 2017-03-29;0(0):. 2017-03-29 [PMID: 28356390] (Bioassay, Mouse)
• J C Neil Runx1 Orchestrates Sphingolipid Metabolism and Glucocorticoid Resistance in Lymphomagenesis J. Cell. Biochem., 2017-01-10;0(0):. 2017-01-10 [PMID: 27869314] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Ifnb1
• Uniprot:
  • P01575()