Recombinant Mouse IFN-beta Protein

Images

 
1 μg/lane of Recombinant Mouse IFN-beta (Catalog # 8234-MB) wasresolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions andvisualized by silver staining, showing bands at 32.7 and 32.9kDa, ...read more
Recombinant Mouse IFN-beta (Catalog # 8234-MB) suppresses viral activity using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus.The ED50 for this effect is 1-6 pg/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Mouse IFN-beta Protein Summary

Additional Information
S.A approximately 1.2x10^9 IU/mg compared to WHO standard
Details of Functionality
Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED50 for this effect is 1-6 pg/mL.

The specific activity of recombinant Mouse IFN‑ beta is approximately 1.2 x 109 IU/mg, which is calibrated against Murine IFN‑ beta WHO International Standard. The Murine IFN‑ beta WHO International Standard (NR-3079) was obtained through the NIH Biodefense and Emerging Infections Research Resources Repository, NIAID, NIH.
Source
Human embryonic kidney cell, HEK293-derived mouse IFN-beta protein
Ile22-Asn182
Accession #
N-terminal Sequence
Ile22
Structure / Form
Monomer
Protein/Peptide Type
Recombinant Proteins
Gene
Ifnb1
Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
30-38 kDa, reducing conditions
Publications
Read Publications using
8234-MB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and Tween® 80 with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IFN-beta Protein

  • Fibroblast interferon
  • IFBIFNB
  • IFF
  • IFNB1
  • IFNbeta
  • IFN-beta
  • interferon beta
  • interferon, beta 1, fibroblast
  • MGC96956

Background

Interferon beta (IFN-beta ), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules (1). Mature mouse IFN-beta shares 75% and 47% amino acid sequence identity with the rat and human proteins, respectively. Fibroblasts are the major producers of IFN-beta, but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogens (2). It is transcriptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3, 4). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) (5). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS (6-10). IFN-beta can additionally induce the expression of the anti-inflammatory cytokine IL-10 (11).
  1. González-Navajas, J.M. et al. (2012) Nat. Rev. Immunol. 12:125.
  2. Reder, A.T. and X. Feng (2013) Front. Immunol. 4:281.
  3. Schafer, S.L. et al. (1998) J. Biol. Chem. 273:2714.
  4. Häcker, H. et al. (2006) Nature 439:204.
  5. Teige, I. et al. (2003) J. Immunol. 170:4776.
  6. Shinohara, M.L. et al. (2008) Immunity 29:68.
  7. Guo, B. et al. (2008) J. Clin. Invest. 118:1680.
  8. Ramgolam, V.S. and S. Markovic-Plese (2010) Endocr. Metab. Immune Disord. Drug Targets 10:161.
  9. Martín-Saavedra, F.M. et al. (2008) Mol. Immunol. 45:4008.
  10. Inoue, M. and M.L. Shinohara (2013) Immunology 139:11.
  11. Wang, H. et al. (2011) J. Immunol 186:675.

Customers Who Viewed This Item Also Viewed...

NBP2-75930
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-24875
Species: Hu, Mu, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
NB100-56566
Species: Hu, Mu, Rt, Po, Bv, Ma
Applications: WB, ChIP, DB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, B/N, CyTOF-ready, ELISA(Cap), Flow-CS, Flow-IC
NB100-56583
Species: Hu, Rb
Applications: WB, Flow, IHC-P, KO
NB120-13810
Species: Hu, Mu, Rt, Po
Applications: WB, Simple Western, IB, ICC/IF, IP
NBP2-67741
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-48011
Species: Hu, Ca, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-67330
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP2-45678
Species: Hu
Applications: WB, IHC, IF
NBP2-29328
Species: Hu, Mu, Rt, Xp, Ye, Ze
Applications: ELISA
NB100-56722
Species: Hu, Mu, Rt, Ca
Applications: WB, DB, ELISA, Flow, Func, ICC/IF, IP, In vitro, B/N, Flow-CS
NBP2-22380
Species: Hu
Applications: WB, IHC, IHC-P

Publications for IFN-beta (8234-MB)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: Bioassay, ELISA (Standard), In Vivo.


Filter By Application
Bioassay
(5)
ELISA (Standard)
(1)
In Vivo
(1)
All Applications
Filter By Species
Mouse
(6)
All Species
Showing Publications 1 - 7 of 7.
Publications using 8234-MB Applications Species
SW Wong, BW Huang, X Hu, E Ho Kim, JP Kolb, RJ Padilla, P Xue, L Wang, TH Oguin, PA Miguez, HC Tseng, CC Ko, J Martinez Global deletion of Optineurin results in altered type I IFN signaling and abnormal bone remodeling in a model of Paget&#039;s disease Cell Death Differ., 2019;0(0):. 2019 [PMID: 31076632] (Bioassay, Mouse) Bioassay Mouse
AM Giebel, S Hu, K Rajaram, R Finethy, E Toh, JA Brothwell, SG Morrison, RJ Suchland, BD Stein, J Coers, RP Morrison, DE Nelson Genetic Screen in Chlamydia muridarum Reveals Role for an Interferon-Induced Host Cell Death Program in Antimicrobial Inclusion Rupture MBio, 2019;10(2):. 2019 [PMID: 30967464] (Bioassay, Mouse) Bioassay Mouse
A Singanayag, N Glanville, JL Girkin, YM Ching, A Marcellini, JD Porter, M Toussaint, RP Walton, LJ Finney, J Aniscenko, J Zhu, MB Trujillo-T, MA Calderazzo, C Grainge, SL Loo, PC Veerati, PS Pathinayak, KS Nichol, AT Reid, PL James, R Solari, PAB Wark, DA Knight, MF Moffatt, WO Cookson, MR Edwards, P Mallia, NW Bartlett, SL Johnston Corticosteroid suppression of antiviral immunity increases bacterial loads and mucus production in COPD exacerbations Nat Commun, 2018;9(1):2229. 2018 [PMID: 29884817] (In Vivo, Mouse) In Vivo Mouse
P Mukherjee, G Hough, A Chattopadh, M Navab, HR Fogelman, D Meriwether, K Williams, S Bensinger, T Moller, KF Faull, AJ Lusis, L Iruela-Ari, KI Bostrom, P Tontonoz, ST Reddy, AM Fogelman Transgenic Tomatoes Expressing the 6F Peptide and Ezetimibe Prevent Diet-induced Increases of Interferon-? and Cholesterol 25-hydroxylase in Jejunum J. Lipid Res., 2017;0(0):. 2017 [PMID: 28592401] (ELISA (Standard)) ELISA (Standard)
A Hunger, RF Medrano, DB Zanatta, PR Del Valle, CA Merkel, TA Salles, DG Ferrari, TK Furuya, SO Bustos, R de Freitas, E Costanzi-S, BE Strauss Reestablishment of p53/Arf and interferon-? pathways mediated by a novel adenoviral vector potentiates antiviral response and immunogenic cell death Cell Death Discov, 2017;3(0):17017. 2017 [PMID: 28386458] (Bioassay, Mouse) Bioassay Mouse
Y Xiao, Q Zou, X Xie, T Liu, HS Li, Z Jie, J Jin, H Hu, G Manyam, L Zhang, X Cheng, H Wang, I Marie, DE Levy, SS Watowich, SC Sun The kinase TBK1 functions in dendritic cells to regulate T cell homeostasis, autoimmunity, and antitumor immunity J. Exp. Med., 2017;0(0):. 2017 [PMID: 28356390] (Bioassay, Mouse) Bioassay Mouse
J C Neil Runx1 Orchestrates Sphingolipid Metabolism and Glucocorticoid Resistance in Lymphomagenesis J. Cell. Biochem., 2016;0(0):. 2016 [PMID: 27869314] (Bioassay, Mouse) Bioassay Mouse

Reviews for IFN-beta (8234-MB) (0)

There are no reviews for IFN-beta (8234-MB). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for IFN-beta (8234-MB) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IFN-beta Products

Bioinformatics Tool for IFN-beta (8234-MB)

Discover related pathways, diseases and genes to IFN-beta (8234-MB). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IFN-beta (8234-MB)

Discover more about diseases related to IFN-beta (8234-MB).
 

Pathways for IFN-beta (8234-MB)

View related products by pathway.

PTMs for IFN-beta (8234-MB)

Learn more about PTMs related to IFN-beta (8234-MB).

Blogs on IFN-beta.

STING in Innate Immunity and Cancer: What’s the Buzz About?
STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA. Bacteria/Virus or self-derived DNA in the cytosol activates the STING pathway and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING also...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse IFN-beta Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol Ifnb1
Uniprot