Recombinant Mouse IFN-beta Protein


1 μg/lane of Recombinant Mouse IFN-beta (Catalog # 8234-MB) wasresolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions andvisualized by silver staining, showing bands at 32.7 and 32.9kDa, more
Recombinant Mouse IFN-beta (Catalog # 8234-MB) suppresses viral activity using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus.The ED50 for this effect is 1-6 pg/mL.

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Recombinant Mouse IFN-beta Protein Summary

Additional Information
S.A approximately 1.2x10^9 IU/mg compared to WHO standard
Details of Functionality
Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED50 for this effect is 1-6 pg/mL.

The specific activity of recombinant Mouse IFN‑ beta is approximately 1.2 x 109 IU/mg, which is calibrated against Murine IFN‑ beta WHO International Standard. The Murine IFN‑ beta WHO International Standard (NR-3079) was obtained through the NIH Biodefense and Emerging Infections Research Resources Repository, NIAID, NIH.
Human embryonic kidney cell, HEK293-derived mouse IFN-beta protein
Accession #
N-terminal Sequence
Structure / Form
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE with silver staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
30-38 kDa, reducing conditions
Read Publications using
8234-MB in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS and Tween® 80 with BSA as a carrier protein.
>95%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IFN-beta Protein

  • Fibroblast interferon
  • IFF
  • IFNB1
  • IFNbeta
  • IFN-beta
  • interferon beta
  • interferon, beta 1, fibroblast
  • MGC96956


Interferon beta (IFN-beta ), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules (1). Mature mouse IFN-beta shares 75% and 47% amino acid sequence identity with the rat and human proteins, respectively. Fibroblasts are the major producers of IFN-beta, but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogens (2). It is transcriptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3, 4). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) (5). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS (6-10). IFN-beta can additionally induce the expression of the anti-inflammatory cytokine IL-10 (11).
  1. González-Navajas, J.M. et al. (2012) Nat. Rev. Immunol. 12:125.
  2. Reder, A.T. and X. Feng (2013) Front. Immunol. 4:281.
  3. Schafer, S.L. et al. (1998) J. Biol. Chem. 273:2714.
  4. Häcker, H. et al. (2006) Nature 439:204.
  5. Teige, I. et al. (2003) J. Immunol. 170:4776.
  6. Shinohara, M.L. et al. (2008) Immunity 29:68.
  7. Guo, B. et al. (2008) J. Clin. Invest. 118:1680.
  8. Ramgolam, V.S. and S. Markovic-Plese (2010) Endocr. Metab. Immune Disord. Drug Targets 10:161.
  9. Martín-Saavedra, F.M. et al. (2008) Mol. Immunol. 45:4008.
  10. Inoue, M. and M.L. Shinohara (2013) Immunology 139:11.
  11. Wang, H. et al. (2011) J. Immunol 186:675.

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Publications for IFN-beta (8234-MB)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: Bioassay, ELISA (Standard), In Vivo.

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Showing Publications 1 - 7 of 7.
Publications using 8234-MB Applications Species
SW Wong, BW Huang, X Hu, E Ho Kim, JP Kolb, RJ Padilla, P Xue, L Wang, TH Oguin, PA Miguez, HC Tseng, CC Ko, J Martinez Global deletion of Optineurin results in altered type I IFN signaling and abnormal bone remodeling in a model of Paget&#039;s disease Cell Death Differ., 2019;0(0):. 2019 [PMID: 31076632] (Bioassay, Mouse) Bioassay Mouse
AM Giebel, S Hu, K Rajaram, R Finethy, E Toh, JA Brothwell, SG Morrison, RJ Suchland, BD Stein, J Coers, RP Morrison, DE Nelson Genetic Screen in Chlamydia muridarum Reveals Role for an Interferon-Induced Host Cell Death Program in Antimicrobial Inclusion Rupture MBio, 2019;10(2):. 2019 [PMID: 30967464] (Bioassay, Mouse) Bioassay Mouse
A Singanayag, N Glanville, JL Girkin, YM Ching, A Marcellini, JD Porter, M Toussaint, RP Walton, LJ Finney, J Aniscenko, J Zhu, MB Trujillo-T, MA Calderazzo, C Grainge, SL Loo, PC Veerati, PS Pathinayak, KS Nichol, AT Reid, PL James, R Solari, PAB Wark, DA Knight, MF Moffatt, WO Cookson, MR Edwards, P Mallia, NW Bartlett, SL Johnston Corticosteroid suppression of antiviral immunity increases bacterial loads and mucus production in COPD exacerbations Nat Commun, 2018;9(1):2229. 2018 [PMID: 29884817] (In Vivo, Mouse) In Vivo Mouse
P Mukherjee, G Hough, A Chattopadh, M Navab, HR Fogelman, D Meriwether, K Williams, S Bensinger, T Moller, KF Faull, AJ Lusis, L Iruela-Ari, KI Bostrom, P Tontonoz, ST Reddy, AM Fogelman Transgenic Tomatoes Expressing the 6F Peptide and Ezetimibe Prevent Diet-induced Increases of Interferon-? and Cholesterol 25-hydroxylase in Jejunum J. Lipid Res., 2017;0(0):. 2017 [PMID: 28592401] (ELISA (Standard)) ELISA (Standard)
A Hunger, RF Medrano, DB Zanatta, PR Del Valle, CA Merkel, TA Salles, DG Ferrari, TK Furuya, SO Bustos, R de Freitas, E Costanzi-S, BE Strauss Reestablishment of p53/Arf and interferon-? pathways mediated by a novel adenoviral vector potentiates antiviral response and immunogenic cell death Cell Death Discov, 2017;3(0):17017. 2017 [PMID: 28386458] (Bioassay, Mouse) Bioassay Mouse
Y Xiao, Q Zou, X Xie, T Liu, HS Li, Z Jie, J Jin, H Hu, G Manyam, L Zhang, X Cheng, H Wang, I Marie, DE Levy, SS Watowich, SC Sun The kinase TBK1 functions in dendritic cells to regulate T cell homeostasis, autoimmunity, and antitumor immunity J. Exp. Med., 2017;0(0):. 2017 [PMID: 28356390] (Bioassay, Mouse) Bioassay Mouse
J C Neil Runx1 Orchestrates Sphingolipid Metabolism and Glucocorticoid Resistance in Lymphomagenesis J. Cell. Biochem., 2016;0(0):. 2016 [PMID: 27869314] (Bioassay, Mouse) Bioassay Mouse

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STING in Innate Immunity and Cancer: What’s the Buzz About?
STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA. Bacteria/Virus or self-derived DNA in the cytosol activates the STING pathway and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING also...  Read full blog post.

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Gene Symbol Ifnb1