Recombinant Mouse IFN-alpha 4 Protein, CF

Recombinant Mouse IFN-alpha/IFNA4 (Catalog # 10259-IF) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is

2 μg/lane of Recombinant Mouse IFN-alpha 4 (Catalog # 10259-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 17-25 kDa.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse IFN-alpha 4 Protein, CF Summary

• Details of Functionality: Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED₅₀ for this effect is <60 pg/mL.
• Source: Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 4 protein
  Leu23-Glu186
• Accession #: P07351
• N-terminal Sequence: Leu23
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 17-25 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IFN-alpha 4 Protein, CF

• IFNA4
• IFNalpha 4
• IFN-alpha 4
• IFN-alpha-4
• IFN-alpha4a
• INFA4
• interferon alpha-4
• Interferon alpha-4B
• Interferon alpha-76
• Interferon alpha-M1
• interferon, alpha 4
• MGC142200

Background

The interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, and are classified based on their binding specificity to cell surface receptors (1). The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 ( alpha -subunit) and IFNAR2 ( beta -subunit). This binding contributes to TNF-alpha induced signaling (2, 3). Both the human and mouse genome code for more than a dozen closely related IFN-alpha subtypes and the various IFN-alphas share about 80% sequence homology among them (4, 5). The mouse IFN-alpha 4 consists of 186 amino acids (aa) including a 24 aa signal peptide and a 162 aa IFN-alpha 4 mature domain. The mature mouse IFN-alpha 4 shares 58% and 76% sequence identity with mature human and rat IFN-alpha 4, respectively. The mouse IFN-alpha 4 reduces the local replication of murine cytomegalovirus in the tibia muscle (6). mIFN-A4 is a strong activator of Mx gene and  was shown to be very effective in reducing splenomegaly (7).

1. Pestka S, et al. (1987) Annu Rev Biochem. 56:727.
2. Fung, K.Y. et al. (2013) Science 339:1088.
3. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
4. Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
5. van Pesch, V. et al. (2004) J. Virol. 78:8219.
6. Yeow, W.S. et al. (1998) J. Immunol. 15:2932.
7. Gerlach, N. et al. (2009) European J. Immunol. 39:136.

Publications for IFN-alpha 4 (10259-IF)(2)

Publications using 10259-IF

• Ruffenach, G;Medzikovic, L;Aryan, L;Sun, W;Lertpanit, L;O'Connor, E;Dehghanitafti, A;Hatamnejad, MR;Li, M;Reddy, ST;Eghbali, M; Intestinal IFN?4 promotes 15-HETE diet-induced pulmonary hypertension Respiratory research 2024-11-28 [PMID: 39609844] (Bioassay, Rat)
• D Kwart, J He, S Srivatsan, C Lett, J Golubov, EM Oswald, P Poon, X Ye, J Waite, AG Zaretsky, S Haxhinasto, E Au-Yeung, NT Gupta, J Chiu, C Adler, S Cherravuru, E Malahias, N Negron, K Lanza, A Coppola, M Ni, H Song, Y Wei, GS Atwal, L Macdonald, NS Oristian, W Poueymirou, V Jankovic, M Fury, I Lowy, AJ Murphy, MA Sleeman, B Wang, D Skokos Cancer cell-derived type I interferons instruct tumor monocyte polarization Cell Reports, 2022-12-06;41(10):111769. 2022-12-06 [PMID: 36476866] (Bioassay, Mouse)

Bioinformatics

• Uniprot:
  • P07351()