Recombinant Mouse HPRG Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse HPRG Protein, CF Summary

• Details of Functionality: Measured by its ability to support the adhesion of MOLT‑4 human acute lymphoblastic leukemia cells. Lamb-Wharton, R.J. and W.T. Morgen (1993) Cell Immunol. 152:544. When 1 x 10⁵ cells/well are added to Recombinant Mouse HPRG coated plates, adhesion is induced in a dose-dependent manner in the presence of Concanvalin A. The ED₅₀ for this effect is 0.6-3 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse HPRG protein
  Leu19-Lys525, with a C-terminal 10-His tag
• Accession #: BAB33094
• N-terminal Sequence: Leu19
• Protein/Peptide Type: Recombinant Enzymes
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 58.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 80-85 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse HPRG Protein, CF

• DKFZp779H1622
• Histidine-proline-rich glycoprotein
• histidine-rich glycoprotein
• HPRG
• HPRGHRGP
• HRG

Background

Mouse histidine-rich glycoprotein (HPRG) is a multidomain, monomeric, secreted, 67 - 75 kDa member of the cystatin superfamily of molecules (1, 2). Its name derives from the fact that 22% of its amino acids (aa) are histidine and proline. In mouse, it is synthesized as a 525 amino acid (aa) precursor that contains an 18 aa signal sequence and a 507 aa mature region (3). Five distinct domains are recognized in the mature molecule. There are two N-terminal cystatin-like modules and one His-Pro-rich region that is flanked by two Pro-rich segments (3, 4). The His-Pro-rich region contains multiple tandem repeats with a GHHPH motif, while the N- and C-termini are linked by a disulfide bond (3, 5, 6). Mouse HPRG is only 60% aa identical to human HPRG, and 79% aa identical to rat HPRG. There are multiple ligands for HPRG. These include small molecular weight molecules (metal ions; heme), hemostatic molecules (heparan sulfate; TSP; plasminogen), and immune system components (T cells; macrophages) (1, 5). About 50% of plasma plasminogen circulates bound to HPRG. Upon immobilization to cell surface tropomyosin in a Zn⁺⁺‑dependent manner, it is converted to plasmin by tPA (7 - 9). HPRG also shows antiangiogenic activity on endothelial cells (10). Finally, it binds to cytoplasmic ligand(s) exposed during cellular necrosis, and facilitates macrophage phagocytosis (11).

1. Jones, A.L. et al. (2005) Immunol. Cell Biol. 83:106.
2. Koide, T. and S. Odani (1987) FEBS Lett. 216:17.
3. Hulett, M.D. and C.R. Parish (2000) Immunol. Cell Biol. 78:280.
4. GenBank Accession # NP_444406.
5. Borza, D-B. et al. (1996) Biochemistry 35:1925.
6. Sorensen, C.B. et al. (1993) FEBS Lett. 328:285.
7. Donate, F. et al. (2004) Cancer Res. 64:5812.
8. Borza, D-B. and W.T. Morgan (1997) J. Biol. Chem. 272:5718.
9. Guan, X. et al. (2004) Thromb. Haemost. 92:403.
10. Juarez, J.C. et al. (2002) Cancer Res. 62:5344.
11. Jones, A.L. et al. (2005) J. Biol. Chem. 280:35733.

Bioinformatics

• Uniprot:
  • BAB33094()