Recombinant Mouse Hip Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse Hip Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit Sonic Hedgehog (Shh) induction of alkaline phosphatase production in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 1.5-7.5 µg/mL in the presence of 5 µg/mL rmShh.
Source
Mouse myeloma cell line, NS0-derived mouse Hip protein
Mouse Hip
(Lys24 - Arg678 & Asp52 - Arg678)
Accession # AAD31172
IEGRMDGGGSGGGSGGGS 10-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Lys24 & Asp52
Protein/Peptide Type
Recombinant Proteins
Gene
Hhip
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
76 kDa and 73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
80-90 kDa and 58-64 kDa, reducing conditions
Publications
Read Publications using
1568-HP/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Hip Protein, CF

  • FLJ90230
  • hedgehog interacting protein
  • hedgehog-interacting protein
  • HHIP
  • Hip
  • HIPFLJ20992

Background

Hedgehog signaling proteins act as mitogens, morphogens, or inducing factors in many different cell types during embryonic development. They aid in growth, patterning, and morphogenesis in both vertebrates and insects (1). Hip (Hedgehog-interacting protein) is a type I transmembrane protein identified for its ability to bind biologically active Sonic Hedgehog. It is comprised of 700 amino acids (aa) and includes a hydrophobic signal sequence, two EGF-like domains near the C-terminus, and a 22 aa membrane-spanning region at the C-terminal end (2). Hip has only been identified in vertebrates and binds all three mammalian Hedgehogs: sonic (Shh), desert (Dhh), and Indian (Ihh). Like the Hedgehog receptor Patched, Hip is a transcriptional target of Hedgehog signaling (2). Unlike Patched, Hip’s ability to bind hedgehogs is not involved in transducing a signal intracellularly, rather it regulates the availability of Hedgehog ligand extracellularly (3). Transgenic mice overexpressing Hip in proliferating chondrocytes display skeletal defects similar to those observed in Ihh mutant mice. These results indicate that Hip is involved in attenuating Hedgehog signaling (2). The expression pattern of Hip correlates with its ability to interact with all three mammalian Hedgehogs. It is expressed in a variety of organs, adjacent with sites of hedgehog expression. For instance, Shh is expressed in the epithelium of the lung, and Hip is found in the underlying lung mesenchyme (2). In fact, Hip knock-out mice exhibit neonatal lethality with respiratory failure due to defective branching morphogenesis. This phenotype correlates with altered expression of Shh markers suggesting an increase in Shh signaling (3). Interestingly, other developmental mechanisms that rely on normal Shh signaling, such as dorsal-ventral patterning of the neural tube, development of the somites, and organ laterality appeared histologically normal in Hip-/- mice (3). Mouse and human Hip share 94% aa identity through the entire protein sequence (4). R&D Systems’ recombinant Hedgehog-interacting protein is a potent antagonist of Shh signaling in vitro.

  1. Ingham, P.W. and A.P. McMahon (2001) Genes & Dev 15:3059.
  2. Chuang, P-T. and A.P. McMahon (1999) Nature 397: 17.
  3. Chuang, P-T. et al. (2003) Genes & Dev. 17:342.
  4. Bak, M. et al. (2001) Cytogenet. Cell Genet. 92:300.

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Publications for Hip (1568-HP/CF)(3)

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Bioinformatics

Gene Symbol Hhip
Entrez
Uniprot