Recombinant Mouse GDF-7 Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse GDF-7 Protein, CF Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.25-1.25 µg/mL.
Source
E. coli-derived mouse GDF-7/BMP-12 protein
Thr308-Arg453
Accession #
N-terminal Sequence
Thr308
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Gdf7
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
15 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
28 kDa, non-reducing conditions
Publications
Read Publications using
779-G7/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse GDF-7 Protein, CF

  • BMP12
  • BMP-12
  • GDF7
  • GDF-7
  • growth differentiation factor 7
  • growth/differentiation factor 7

Background

Growth Differentiation Factor 7 (GDF-7), also known as bone morphogenetic protein (BMP) 12 or cartilage derived morphogenetic protein 3 (CDMP-3), is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor  beta (TGF-beta ) superfamily. Mature GDF-7 contains 146 amino acid (aa) residues with the characteristic seven conserved cysteine residues. The bioactive GDF-7 is a homodimer with an apparent molecular weight of approximately 28 kDa. Mature GDF-5, GDF-6 and GDF-7, which share 80 - 86% identity with each other, define a new subgroup within the BMP family. Like other TGF-beta superfamily proteins, GDF-7 is highly conserved across species. At the amino acid sequence level, mature human and mouse GDF-7 are 98% identical. It has been reported that GDF-7 has multiple functions including regulation of chondrogenesis, bone morphogenesis, and neuron differentiation. GDF-7 response is mediated by the formation of hetero-oligomeric complexes of type I (BMPR-IB) and type II (BMPR-II or Activin R-II) sereine/threonine kinase receptors, and the activiation of Smad proteins.

  1. Storm, E.E., et al. (1994) Nature 368:639.
  2. Nishitoh, H., et al. (1996) J. Biol. Chem. 271:21345.
  3. Francis-West, P.H., et al. (1999) Development 126:1035.
  4. Massague, J. et al. (2000) Genes and Dev. 14:627.

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Publications for GDF-7/BMP-12 (779-G7/CF)(3)

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Bioinformatics

Gene Symbol Gdf7
Entrez
Uniprot