Recombinant Mouse FOLR2 Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse FOLR2 Protein, CF Summary

Details of Functionality
Measured by its ability to bind Folic Acid-Bovine Serum Albumin with an estimated Kd < 1.6 nM.
Source
Mouse myeloma cell line, NS0-derived mouse FOLR2 protein
Met-Ser227, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Arg21
Protein/Peptide Type
Recombinant Proteins
Gene
Folr2
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
25.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
30-37 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse FOLR2 Protein, CF

  • beta-HFR
  • FBP
  • FBP/PL-1
  • folate receptor 2 (fetal)
  • Folate receptor 2
  • folate receptor beta
  • folate receptor, beta
  • Folate receptor, fetal/placental
  • folate-binding protein, fetal/placental
  • FOLR2
  • FR-beta
  • FR-P3
  • Placental folate-binding protein

Background

Folate Receptor 2 (FOLR2), also known as Folate Receptor beta, is a 38 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR2 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3 - 5). Mouse FOLR2 shares 82% and 91% amino acid sequence identity with human and rat FOLR2, respectively. FOLR2 is predominantly expressed in placenta, cells of the neutrophilic lineage, and some CD34+ hematopoietic progenitor cells (4, 6, 7). It is up‑regulated on myeloid  leukemias, head and neck squamous cell carcinomas, and several nonepithelial cancers (3, 6, 8). It is also up‑regulated on immunosuppressive tumor-associated macrophages as well as on macrophages and monocytes at chronic inflammatory sites including rheumatoid arthritis synovium and glioblastoma (9 - 12). FOLR2 knockout mice do not show gross morphological defects, but they exhibit increased sensitivity to arsenate-induced teratogenicity (13, 14).
  1. Fowler, B. et al. (2001) Kidney Int. 59:S-221.
  2. Kelemen, L.E. (2006) Int. J. Cancer 119:243.
  3. Shen, F. et al. (1994) Biochemistry 33:1209.
  4. Ratnam, M. et al. (1989) Biochemistry 28:8249.
  5. Brigle, K.E. et al. (1991) J. Biol. Chem. 266:17243.
  6. Ross, J.F. et al. (1999) Cancer 85:348.
  7. Reddy, J.A. et al. (1999) Blood 93:3940.
  8. Ross, J.F. et al. (1994) Cancer 73:2432.
  9. Puig-Kroger, A. et al. (2009) Cancer Res. 69:9395.
  10. Nakashima-Matsushita, N. et al. (1999) Arthritis Rheum. 42:1609.
  11. van der Heijden, J.W. et al. (2009) Arthritis Rheum. 60:12.
  12. Nagai, T. et al. (2009) Cancer Immunol. Immunother. 58:1577.
  13. Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
  14. Wlodarczyk, B. et al. (2001) Toxicol. Appl. Pharmacol. 177:238.

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Bioinformatics

Gene Symbol Folr2
Uniprot