Recombinant Mouse Fc alpha/mu R His-tag Protein, CF

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When Recombinant Mouse Fc alpha/mu R (Catalog # 10069-FC) is coated at 2ug/mL, 100 uL/well, Mouse IgM binds with an ED50 of 0.04-0.24 μg/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse Fc alpha/mu R His-tag Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Fc alpha /μ R is immobilized at 2 μg/mL, 100 μL/well, the concentration of Mouse IgM that produces 50% of the optimal binding response is 0.04-0.24 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Fc alpha/mu R protein
Trp47-Arg455, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Trp47
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
97-112 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Fc alpha/mu R His-tag Protein, CF

  • CD351 antigen
  • CD351
  • Fc alpha/mu R
  • Fc alpha/mu receptor
  • Fc receptor, IgA, IgM, high affinity
  • FCA/MR
  • FCAMR
  • FKSG87
  • high affinity immunoglobulin alpha and immunoglobulin mu Fc receptor
  • immunity related factor
  • receptor for Fc fragment of IgA and IgM
  • uR

Background

Fc alpha/mu Receptor (FCAMR), designated CD351, is an approximately 60-kDa transmembrane protein that serves as a receptor for IgA and IgM immunoglobulins (1). Mature mouse FCAMR consists of a 420 amino acid (aa) extracellular domain with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 59 aa cytoplasmic domain. Within the ECD, mouse FCAMR shares 51% aa and 80% aa sequence identity with human and rat FCAMR, respectively. FCAMR is expressed on B cells, macrophages, and kidney mesangial cells (2-4). It binds to both IgA and IgM in immune complexes but not to monomeric immunoglobulin (2, 5). FCAMR participates in pathogen clearance as well as foam cell formation by mediating the internalization of IgM-opsonized microbes and oxidized LDL-containing particles (2, 6).
  1. Wang, H. et al. (2016) Front. Immunol. 7:99.
  2. Shibuya, A. et al. (2000) Nat. Immunol. 1:441.
  3. Matesanz-Isabel, J. et al. (2011) Immunol. Lett. 134:104.
  4. McDonald, K.J. et al. (2002) Biochem. Biophys. Res. Commun. 290:438.
  5. Ghumra, A. et al. (2009) Eur. J. Immunol. 39:1147.
  6. Feng, X. et al. (2010) Atherosclerosis 208:396.

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