Recombinant Mouse Epigen Protein

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse Epigen Protein Summary

• Additional Information: U.S. Patent No. 6,150,502
• Details of Functionality: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED₅₀ for this effect is 50-150 ng/mL.
• Source: E. coli-derived mouse Epigen protein
  Leu53-Ala103
• Accession #: Q924X1.1
• N-terminal Sequence: Leu53
• Protein/Peptide Type: Recombinant Proteins
• Gene: Epgn
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Theoretical MW: 5.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

U.S. Patent No. 6,150,502 used under license from Genesis Research and Development Corporation Limited.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Epigen Protein

• EPGN
• Epigen

Background

Epigen is an eipthelial mitogen that belongs to the EGF superfamily (1). Mouse Epigen cDNA encodes a 153 amino acid (aa) residue type I transmembrane precursor protein with a 18 aa putative signal peptide, a 92 aa extracellular domain, a 20 aa transmembrane domain and a 22 aa cytoplasmic domain. Expression of mouse Epigen has been detected in multiple tissues including testis, liver and heart. Over the entire protein, mouse Epigen shares from approximately 23% to 33% sequence homology with other EGF family members. The functional internal peptide containing the EGF motif however is more than 40% homologous with that of TGF-alpha and epiregulin. A human cDNA clone encoding a mouse epigen-like protein has also been identified (2). Similarly to other EGF family proteins, a soluble form of mouse Epigen can most likely be generated from the membrane-bound form by proteolytic cleavage. Although recombinant mouse Epigen has been shown to be mitogenic for fibroblasts (3) and epithelial cells (1), compared to EGF and TGF-alpha, it is much less potent. The EGF receptor ErbB1 can be activated upon mouse Epigen activation. Nevertheless, involvements of additional ErbB receptors in mouse Epigen signaling have not been ruled out.

1. Strachan, L. et al. (2001) J. Biol. Chem. 276:18265.
2. Accession XM_171078.
3. R&D Systems, unpublished results.

Publications for Epigen (1127-EP)(1)

Publication using 1127-EP

• Stoll SW, Johnson JL, Li Y, Rittie L, Elder JT Amphiregulin carboxy-terminal domain is required for autocrine keratinocyte growth. J. Invest. Dermatol., 2010;130(8):2031-40. 2010 [PMID: 20428186] (Bioassay, Human)

Bioinformatics

• Gene Symbol: Epgn
• Entrez:
  • 255324(Human)
  • 71920(Mouse)
• Uniprot:
  • Q924X1.1()