Recombinant Mouse EphA3 Fc Chimera Protein, CF


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Product Details

Reactivity MuSpecies Glossary
Applications Binding Activity

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Recombinant Mouse EphA3 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized rmEphA3/Fc Chimera at 2 µg/mL (100 µL/well) can bind rhEphrin A5 Fc Chimera with a linear range of 0.08-5 ng/mL. Optimal dilutions should be determined by each laboratory for each application.
Mouse myeloma cell line, NS0-derived mouse EphA3 protein
Mouse EphA3
Glu21 - His541 (Thr323Ala & Glu476Gln)
Accession # Q8BRB1
(Pro100 - Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


  • Binding Activity
Theoretical MW
86 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
110 kDa, reducing conditions
Read Publications using
640-A3 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Tris.
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse EphA3 Fc Chimera Protein, CF

  • Cek4
  • EC 2.7.10
  • EC
  • EK4
  • EPH receptor A3
  • EphA3
  • EPH-like kinase 4
  • ephrin type-A receptor 3
  • ETK
  • Hek4
  • HEKETK1eph-like tyrosine kinase 1
  • human embryo kinase 1
  • TYRO4 protein tyrosine kinase
  • Tyro4
  • Tyrosine-protein kinase receptor ETK1
  • Tyrosine-protein kinase TYRO4


EphA3, also known as Cek4, Mek4, Hek, Tyro4, and Hek4, is a 135 kDa glycosylated member of the transmembrane Eph receptor tyrosine kinase family. The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. EphA3 preferentially binds to Ephrin-A5. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1, 2). The 520 amino acid (aa) extracellular domain (ECD) of mouse EphA3 contains an N-terminal Ephrin binding region, a cysteine-rich region, and two fibronectin type II domains. The 419 aa cytoplasmic domain contains the tyrosine kinase domain and a sterile alpha motif (SAM) (3, 4). Within the ECD, mouse EphA3 shares 96% and 98% aa sequence identity with human and rat EphA3, respectively. Alternate splicing generates a secreted isoform that consists of nearly the entire ECD. EphA3 is expressed in the developing forebrain, retinal axons, some spinal cord motor neurons, and the heart where it plays an important role in axonal repulsion and organ morphogenesis (5 ‑ 8). It is upregulated on some hematopoietic and solid tumor cells and on astrocytes surrounding injured nervous tissue (5, 9 - 11). EphA3 ligation inhibits cellular adhesion to fibronectin as well as cellular migration (9, 10). Transmembrane EphA3 associates in cis with ADAM10 which then promotes the cleavage in trans of Ephrin-A5 (12). It also associates in cis with Ephrin-A5 on retinal axons, thereby preventing the activation of EphA3 by Ephrin-A (13). Multiple tyrosine residues within the cytoplasmic region of EphA3 become phosphorylated during ligand-induced signaling (14, 15).
  1. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
  2. Merlos-Suarez, A. and E. Batlle (2008) Curr. Opin. Cell Biol. 20:194.
  3. Sajjadi, F.G. et al. (1991) New Biol. 3:769.
  4. Lackmann, M. et al. (1998) J. Biol. Chem. 273:20228.
  5. Chiari, R. et al. (2000) Cancer Res. 60:4855.
  6. Kudo, C. et al. (2005) J. Comp. Neurol. 487:255.
  7. Kilpatrick, T.J. et al. (1996) Mol. Cell. Neurosci. 7:62.
  8. Stephen, L.J. et al. (2007) Dev. Biol. 302:66.
  9. Smith, L.M. et al. (2004) Exp. Cell Res. 292:295.
  10. Clifford, N. et al. (2008) J. Cell. Biochem. 105:1250.
  11. Irizarry-Ramirez, M. et al. (2005) J. Neurotrauma 22:929.
  12. Janes, P.W. et al. (2005) Cell 123:291.
  13. Carvalho, R.F. et al. (2006) Nat. Neurosci. 9:322.
  14. Shi, G. et al. (2010) Cell Res. June epub.
  15. Hu, T. et al. (2009) Biochemistry 48:6369.

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Publications for EphA3 (640-A3)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 2 applications: Bioassay, Immunoprecipitation.

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Showing Publications 1 - 6 of 6.
Publications using 640-A3 Applications Species
T Itoh, R Hatano, Y Horimoto, T Yamada, D Song, H Otsuka, Y Shirakawa, S Mastuoka, N Iwao, TM Aune, NH Dang, Y Kaneko, K Okumura, C Morimoto, K Ohnuma IL-26 mediates epidermal growth factor receptor-tyrosine kinase inhibitor resistance through endoplasmic reticulum stress signaling pathway in triple-negative breast cancer cells Cell Death & Disease, 2021-05-21;12(6):520. 2021-05-21 [PMID: 34021125] (Bioassay, Mouse) Bioassay Mouse
K Gerstmann, D Pensold, J Symmank, M Khundadze, CA Hübner, J Bolz, G Zimmer Thalamic afferents influence cortical progenitors via ephrin A5-EphA4 interactions Development, 2014-12-05;142(1):140-50. 2014-12-05 [PMID: 25480914] (Bioassay, Mouse) Bioassay Mouse
Ono , Katsuhik, Clavairoly , Adrien, Nomura , Tadashi, Gotoh , Hitoshi, Uno , Aoi, Armant , Olivier, Takebayashi , Hirohide, Zhang , Qi, Shimamura , Kenji, Itohara , Shigeyos, Parras , Carlos M, Ikenaka , Kazuhiro Development of the prethalamus is crucial for thalamocortical projection formation and is regulated by Olig2. Development, 2014-05-01;141(10):2075-84. 2014-05-01 [PMID: 24803655] (Bioassay, Mouse) Bioassay Mouse
Passante L, Gaspard N, Degraeve M, Frisen J, Kullander K, De Maertelaer V, Vanderhaeghen P Temporal regulation of ephrin/Eph signalling is required for the spatial patterning of the mammalian striatum. Development, 2008-08-28;135(19):3281-90. 2008-08-28 [PMID: 18755772] (Bioassay, Mouse) Bioassay Mouse
Zhu Y, Guthrie S, Murakami F Ephrin A/EphA controls the rostral turning polarity of a lateral commissural tract in chick hindbrain. Development, 2006-10-01;133(19):3837-46. 2006-10-01 [PMID: 16968816] (Bioassay, Chicken) Bioassay Chicken
Janes PW, Saha N, Barton WA, Kolev MV, Wimmer-Kleikamp SH, Nievergall E, Blobel CP, Himanen JP, Lackmann M, Nikolov DB Adam meets Eph: an ADAM substrate recognition module acts as a molecular switch for ephrin cleavage in trans. Cell, 2005-10-21;123(2):291-304. 2005-10-21 [PMID: 16239146] (Immunoprecipitation, Human) Immunoprecipitation Human

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    Gene Symbol Epha3