Recombinant Mouse EphA3 Fc Chimera Protein, CF

Images

 
There are currently no images for EphA3 (640-A3).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Mouse EphA3 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized rmEphA3/Fc Chimera at 2 µg/mL (100 µL/well) can bind rhEphrin A5 Fc Chimera with a linear range of 0.08-5 ng/mL. Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse EphA3 protein
Mouse EphA3
Glu21 - His541 (Thr323Ala & Glu476Gln)
Accession # Q8BRB1
IEGRMD Human IgG1
(Pro100 - Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Glu21
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Epha3
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
86 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
110 kDa, reducing conditions
Publications
Read Publications using
640-A3 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse EphA3 Fc Chimera Protein, CF

  • Cek4
  • EC 2.7.10
  • EC 2.7.10.1
  • EK4
  • EPH receptor A3
  • EphA3
  • EPH-like kinase 4
  • ephrin type-A receptor 3
  • ETK
  • Hek4
  • HEKETK1eph-like tyrosine kinase 1
  • human embryo kinase 1
  • TYRO4 protein tyrosine kinase
  • Tyro4
  • Tyrosine-protein kinase receptor ETK1
  • Tyrosine-protein kinase TYRO4

Background

EphA3, also known as Cek4, Mek4, Hek, Tyro4, and Hek4, is a 135 kDa glycosylated member of the transmembrane Eph receptor tyrosine kinase family. The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. EphA3 preferentially binds to Ephrin-A5. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1, 2). The 520 amino acid (aa) extracellular domain (ECD) of mouse EphA3 contains an N-terminal Ephrin binding region, a cysteine-rich region, and two fibronectin type II domains. The 419 aa cytoplasmic domain contains the tyrosine kinase domain and a sterile alpha motif (SAM) (3, 4). Within the ECD, mouse EphA3 shares 96% and 98% aa sequence identity with human and rat EphA3, respectively. Alternate splicing generates a secreted isoform that consists of nearly the entire ECD. EphA3 is expressed in the developing forebrain, retinal axons, some spinal cord motor neurons, and the heart where it plays an important role in axonal repulsion and organ morphogenesis (5 ‑ 8). It is upregulated on some hematopoietic and solid tumor cells and on astrocytes surrounding injured nervous tissue (5, 9 - 11). EphA3 ligation inhibits cellular adhesion to fibronectin as well as cellular migration (9, 10). Transmembrane EphA3 associates in cis with ADAM10 which then promotes the cleavage in trans of Ephrin-A5 (12). It also associates in cis with Ephrin-A5 on retinal axons, thereby preventing the activation of EphA3 by Ephrin-A (13). Multiple tyrosine residues within the cytoplasmic region of EphA3 become phosphorylated during ligand-induced signaling (14, 15).
  1. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
  2. Merlos-Suarez, A. and E. Batlle (2008) Curr. Opin. Cell Biol. 20:194.
  3. Sajjadi, F.G. et al. (1991) New Biol. 3:769.
  4. Lackmann, M. et al. (1998) J. Biol. Chem. 273:20228.
  5. Chiari, R. et al. (2000) Cancer Res. 60:4855.
  6. Kudo, C. et al. (2005) J. Comp. Neurol. 487:255.
  7. Kilpatrick, T.J. et al. (1996) Mol. Cell. Neurosci. 7:62.
  8. Stephen, L.J. et al. (2007) Dev. Biol. 302:66.
  9. Smith, L.M. et al. (2004) Exp. Cell Res. 292:295.
  10. Clifford, N. et al. (2008) J. Cell. Biochem. 105:1250.
  11. Irizarry-Ramirez, M. et al. (2005) J. Neurotrauma 22:929.
  12. Janes, P.W. et al. (2005) Cell 123:291.
  13. Carvalho, R.F. et al. (2006) Nat. Neurosci. 9:322.
  14. Shi, G. et al. (2010) Cell Res. June epub.
  15. Hu, T. et al. (2009) Biochemistry 48:6369.

Customers Who Viewed This Item Also Viewed...

NBP2-22203
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
MAB1230
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC, KO
NB100-56566
Species: Hu, Mu, Rt, Po, Bv, Ma
Applications: WB, ChIP, DB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, B/N, CyTOF-ready, Dual ISH-IHC, ELISA(Cap), Flow-CS, Flow-IC, KD, KO
NBP2-82026
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NB100-56583
Species: Hu, Rt, Rb
Applications: WB, Flow, IHC, IHC-P, KO
NB100-56722
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, DB, ELISA, Flow, Func, ICC/IF, IP, In vitro, B/N, Flow-CS
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
AF2685
Species: Hu
Applications: WB, Simple Western, IHC
NB100-56655
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-13075
Species: Hu, Mu, Rt, Pm
Applications: WB, DB, ELISA, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
AF5887
Species: Hu
Applications: WB, IHC
NBP3-03109
Species: Hu, Mu, Rt
Applications: WB, IHC
AF1280
Species: Mu
Applications: WB, Simple Western, IHC
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-81838
Species: Hu, Mu
Applications: WB, IHC, IHC-P

Publications for EphA3 (640-A3)(5)

We have publications tested in 3 confirmed species: Human, Mouse, Chicken.

We have publications tested in 2 applications: Bioassay, Immunoprecipitation.


Filter By Application
Bioassay
(4)
Immunoprecipitation
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(3)
Chicken
(1)
All Species
Showing Publications 1 - 5 of 5.
Publications using 640-A3 Applications Species
Gerstmann K, Pensold D, Symmank J, Khundadze M, Hubner C, Bolz J, Zimmer G Thalamic afferents influence cortical progenitors via ephrin A5-EphA4 interactions. Development, 2015;142(1):140-50. 2015 [PMID: 25480914] (Bioassay, Mouse) Bioassay Mouse
Ono, Katsuhik, Clavairoly, Adrien, Nomura, Tadashi, Gotoh, Hitoshi, Uno, Aoi, Armant, Olivier, Takebayashi, Hirohide, Zhang, Qi, Shimamura, Kenji, Itohara, Shigeyos, Parras, Carlos M, Ikenaka, Kazuhiro Development of the prethalamus is crucial for thalamocortical projection formation and is regulated by Olig2. Development, 2014;141(10):2075-84. 2014 [PMID: 24803655] (Bioassay, Mouse) Bioassay Mouse
Passante L, Gaspard N, Degraeve M, Frisen J, Kullander K, De Maertelaer V, Vanderhaeghen P Temporal regulation of ephrin/Eph signalling is required for the spatial patterning of the mammalian striatum. Development, 2008;135(19):3281-90. 2008 [PMID: 18755772] (Bioassay, Mouse) Bioassay Mouse
Zhu Y, Guthrie S, Murakami F Ephrin A/EphA controls the rostral turning polarity of a lateral commissural tract in chick hindbrain. Development, 2006;133(19):3837-46. 2006 [PMID: 16968816] (Bioassay, Chicken) Bioassay Chicken
Janes PW, Saha N, Barton WA, Kolev MV, Wimmer-Kleikamp SH, Nievergall E, Blobel CP, Himanen JP, Lackmann M, Nikolov DB Adam meets Eph: an ADAM substrate recognition module acts as a molecular switch for ephrin cleavage in trans. Cell, 2005;123(2):291-304. 2005 [PMID: 16239146] (Immunoprecipitation, Human) Immunoprecipitation Human

Reviews for EphA3 (640-A3) (0)

There are no reviews for EphA3 (640-A3). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for EphA3 (640-A3). (Showing 1 - of FAQs).

    Customers Who Bought This Also Bought

    Contact Information

    Product PDFs

    Calculators

    Concentration Calculator

    The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

    =
    ÷

    Review this Product

    Be the first to review our Recombinant Mouse EphA3 Fc Chimera Protein, CF and receive a gift card or discount.

    Bioinformatics

    Gene Symbol Epha3
    Uniprot