Recombinant Mouse CXCL17/VCC-1 Protein

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse CXCL17/VCC-1 Protein Summary

• Details of Functionality: Measured by its ability to induce VEGF expression in mouse endothelial cells. Weinstein, E.J. et al. (2006) BBRC 350:74. The ED₅₀ for this effect is 1-5 µg/mL.
• Source: E. coli-derived mouse CXCL17/VCC-1 protein
  Ser23-Leu119
• Accession #: Q5UW37.1
• N-terminal Sequence: Ser31
• Protein/Peptide Type: Recombinant Proteins
• Gene: Cxcl17
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 10.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CXCL17/VCC-1 Protein

• Chemokine (C-X-C Motif) Ligand 17
• C-X-C Motif Chemokine 17
• CXCL17
• Dcip1
• Dendritic Cell And Monocyte Chemokine-Like Protein
• DMC
• UNQ473
• VCC1
• VCC-1
• VEGF Coregulated Chemokine 1
• VEGF Co-Regulated Chemokine 1

Background

Dendritic cell and monocyte chemokine-like protein (DMC), also known as VEGF-correlated chemokine-1 (VCC-1), is a secreted molecule with a size and predicted three-dimensional folding pattern similar to that of chemokines CXCL8/IL-8 and CXCL14/BRAK (1, 2). It has no predicted N-glycosylation sites, so cleavage of a 22 amino acid (aa) signal sequence likely results in a mature mouse DMC molecule of 97 aa and 11 kDa size. DMC is constitutively produced by airway and intestinal epithelium (1). It induces the chemotaxis of quiescent, but not LPS‑activated peripheral blood monocytes and dendritic cells, and also binds these cells specifically (1). DMC expression is increased in endothelial cells when they are induced to form tubes in vitro (2). Transgenic overexpression in NIH3T3 cells causes up‑regulation of proteins such as VEGF and FGF basic, and increases cell growth rate and tumorigenicity (2). DMC and two other chemokines that play roles in angiogenesis, CXCL1/GRO and CXCL8/IL-8, show significantly correlated expression with that of VEGF in primary lung, breast and esophageal tumors (2). DMC is therefore suggested to play a role in tumor angiogenesis. Mature mouse DMC shares 82%, 71% and 66% amino acid sequence identity with rat, human and bovine DMC, respectively.

1. Pisabarro, M. T. et al. (2006) J. Immunol. 176:2069.
2. Weinstein, E. J. et al. (2006) Biochem. Biophys. Res. Commun. 350:74.

Publications for CXCL17/VCC-1 (4270-DM)(3)

Publications using 4270-DM

• S Shimada, K Makino, M Jinnin, S Sawamura, Y Kawano, M Ide, I Kajihara, T Makino, S Fukushima, H Ihn CXCL17-mediated downregulation of type I collagen via MMP1 and miR-29 in skin fibroblasts possibly contributes to the fibrosis in systemic sclerosis J Dermatol Sci, 2020-09-29;0(0):. 2020-09-29 [PMID: 33055012] (Bioassay, Mouse)
• YL Hsu, MC Yen, WA Chang, PH Tsai, YC Pan, SH Liao, PL Kuo CXCL17-derived CD11b+Gr-1+ myeloid-derived suppressor cells contribute to lung metastasis of breast cancer through platelet-derived growth factor-BB Breast Cancer Res., 2019-02-12;21(1):23. 2019-02-12 [PMID: 30755260] (Bioassay, In Vivo, Mouse)
• Burkhardt A, Maravillas-Montero J, Carnevale C, Vilches-Cisneros N, Flores J, Hevezi P, Zlotnik A CXCL17 is a major chemotactic factor for lung macrophages. J Immunol, 2014-06-27;193(3):1468-74. 2014-06-27 [PMID: 24973458] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Cxcl17
• Uniprot:
  • Q5UW37.1()