Recombinant Mouse CRELD2 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CRELD2 Protein, CF Summary

• Details of Functionality: Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is typically 0.75-3 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse CRELD2 protein
  Ser23-Leu350, with a C-terminal 6-His tag
• Accession #: Q9CYA0
• N-terminal Sequence: Ser23
• Protein/Peptide Type: Recombinant Proteins
• Gene: Creld2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 36.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 57-62 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CRELD2 Protein, CF

• CRELD2
• cysteine-rich with EGF-like domain protein 2
• cysteine-rich with EGF-like domains 2
• DKFZp667O055
• MGC11256

Background

Cysteine‑rich with EGF‑like domain protein 2 (CRELD2) is an approximately 60 kDa glycoprotein that contains two EGF‑like domains and two FU domains (1). Mature mouse CRELD2 shares approximately 77% and 92% aa sequence identity with human and rat CRELD2, respectively. It is widely expressed in fetal and adult tissues (1, 2). CRELD2 localizes to the endoplasmic reticulum and Golgi and can also be secreted (2‑4). It is up‑regulated during the cellular stress‑induced unfolded protein response (UPR), cartilage and bone pathologies (MED and MCDS), and arsenic‑induced liver toxicity (3, 5‑7). CRELD2 interacts intracellularly with the alpha 4 and beta 2 subunits of the nicotinic acetylcholine receptor and inhibits cell surface expression of the receptor (2, 8). CRELD2 expression is up‑regulated in prostate cancer by dihydroxytestosterone and is down‑regulated following anti‑androgen treatment (9).

1. Rupp, P.A. et al. (2002) Gene 293:47.
2. Ortiz, J.A. et al. (2005) J. Neurochem. 95:1585.
3. Oh-hashi, K. et al. (2009) Biochem. Biophys. Res. Commun. 387:504.
4. Oh-hashi, K. et al. (2011) FEBS Lett. 585:2481.
5. Cameron, T.L. et al. (2011) PLoS ONE 6:e24600.
6. Nundlall, S. et al. (2010) Cell Stress Chaperones 15:835.
7. Nohara, K. et al. (2012) Toxicol. Sci. epub.
8. Hosur, V. et al. (2009) J. Neurochem. 111:848.
9. Jariwala, U. et al. (2007) Mol. Cancer 6:39.

Bioinformatics

• Gene Symbol: Creld2
• Uniprot:
  • Q9CYA0(Mouse)