Recombinant Mouse Contactin-6 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse Contactin-6 Protein, CF Summary

• Details of Functionality: Bioassay data are not available.

• Source: Chinese Hamster Ovary cell line, CHO-derived mouse Contactin-6 protein
  Glu20-Ser998, with a C-terminal 6-His tag
• Accession #: Q9JMB8
• N-terminal Sequence: Glu20
• Protein/Peptide Type: Recombinant Proteins
• Gene: Cntn6
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 109.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 140-160 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Contactin-6 Protein, CF

• CNTN6
• contactin 6
• Contactin6
• Contactin-6
• MGC133256
• NB3

Background

Contactin-6, also known as NB-3, is a 130 kDa member of the TAG/F3 subgroup of Contactin GPI-linked neuronal adhesion proteins (1, 2). Mature mouse Contactin-6 consists of six immunoglobulin-like domains, a flexible linker region, four fibronectin type III domains, and a GPI anchor (3 - 5). Mouse Contactin-6 shares 90% and 96% amino acid (aa) sequence identity with human and rat Contactin-6, respectively. Alternate splicing generates an isoform with a 17 aa deletion in the first Ig-like domain (3). Contactin-6 expression is upregulated postnatally in the cerebellum, brainstem, and hippocampus, and it shows differential expression within various structures of the brain (3, 5 - 7). In the cerebellum, it is presynaptically localized at synapses between excitatory glutamatergic parallel fibers and Purkinje cells (7). Contactin-6 associates in cis with CHL-1 and in trans with Notch-1 on oligodendrocytes (6, 8). Its binding to Notch-1 triggers translocation of the Notch intracellular domain to the nucleus and promotes oligodendrogenesis (6). Contactin-6 function is important in the postnatal development and function of the cerebellum, as shown in knockout mice which exhibit poor motor coordination (5, 7).

1. Shimoda, Y. and K. Watanabe (2009) Cell Adhesion Migration 3:64.
2. Hu, Q.D. et al. (2006) Dev. Neurosci. 28:25.
3. Lee, S. et al. (2000) Gene 245:253.
4. Ogawa, J. et al. (1996) Neurosci. Lett. 218:173.
5. Takeda, Y. et al. (2003) J. Neurobiol. 56:252.
6. Cui, X.-Y. et al. (2004) J. Biol. Chem. 279:25858.
7. Sakurai, K. et al. (2009) Dev. Neurobiol. Aug 11 epub.
8. Ye, H. et al. (2008) EMBO J. 27:188.

Bioinformatics

• Gene Symbol: Cntn6
• Uniprot:
  • Q9JMB8(Mouse)