Recombinant Mouse CD96 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CD96 Protein, CF Summary

• Additional Information: His-tag version
• Details of Functionality: Measured by its binding ability in a functional ELISA.

  When recombinant mouse (rm) CD155 is coated at 5 μg/mL (100 μL/well), the concentration of rmCD96 that produces 50% optimal binding response is found to be approximately 0.015-0.075 μg/mL.

• Source: Mouse myeloma cell line, NS0-derived mouse CD96 protein
  Met1-Met536, with a C-terminal 6-His tag
• Accession #: AAH52865
• N-terminal Sequence: Glu25
• Protein/Peptide Type: Recombinant Proteins
• Gene: Cd96
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 57.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 135-155 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CD96 Protein, CF

• CD96 antigenMGC22596
• CD96 molecule
• CD96
• DKFZp667E2122
• T cell-activated increased late expression protein
• TACTILE
• TACTILEincreased late expression
• T-cell surface protein tactile

Background

Mouse CD96, also known as Tactile (T cell‑activated increased late expression), is a 160 kDa type I transmembrane glycoprotein of the Ig superfamily that shows peak expression 6 ‑ 9 days after activation of T, NK, and a subpopulation of B cells (1, 2). CD96 is also frequently expressed on acute myeloid leukemia and myelodysplastic stem cells (3, 4). Low expression of adhesive human CD96 is a rare cause of C syndrome, a set of developmental anomalies in cranial bone (trigonocephaly), skin and viscera, demonstrating a role for CD96 in development of these tissues (2, 5). Mouse CD96 cDNA encodes 602 amino acids (aa) including a 21 aa signal peptide, a 515 aa extracellular domain (ECD) that contains two V‑type and one C‑type Ig‑like  domain, a 21 aa transmembrane sequence, and a 45 aa cytoplasmic domain (1). Within the ECD, mouse CD96 shares 55% and 79% aa sequence identity with human CD96v2 and rat CD96, respectively (6). The ECD is highly N- and O‑glycosylated (1). Humans, but not mice, express a splice variant with a 16 aa insert in the second V‑type domain called CD96v1 (2). In mice, a truncated, potentially secreted 437 aa isoform is reported (7). An 80 kDa soluble form is elevated in human serum during chronic hepatitis B (8). The most N‑terminal Ig‑like domain of human or mouse CD96 binds to CD155/PVR (poliovirus receptor), but CD96/CD155 interaction is species‑specific (2, 6, 9). Mouse CD96 also binds nectin-1 (6). On NK cells, co‑stimulatory CD96 and DNAM‑1 (CD226) are thought to have paired activity with inhibitory TIGIT, all of which bind CD155 and various nectins (10, 11). CD96 can promote NK cell adhesion to, and killing of target cells, including tumors that express CD155 (9, 10).

1. Wang, P.L. et al. (1992) J. Immunol. 148:2600.
2. Meyer, D. et al. (2009) J. Biol. Chem. 284:2235.
3. Hosen, N. et al. (2007) Proc. Natl. Acad. Sci. USA 104:11008.
4. Xie, W. et al. (2010) Cytometry A 77:840.
5. Kaname, T. et al. (2007) Am. J. Hum. Genet. 81:835.
6. Seth, S. et al. (2007) Biochem. Biophys. Res. Commun. 364:959.
7. Protein Accession # BAE32358.
8. Gong, J. et al. (2008) Clin. Exp. Immunol. 155:207.
9. Fuchs, A. et al. (2004) J. Immunol. 172:3394.
10. Stanietsky, N. and O. Mandelboim (2010) FEBS Lett. 584:4895.
11. Xu, Z. and B. Jin (2010) Cell. Mol. Immunol. 7:11.

Publications for CD96 (5690-CD)(1)

Publication using 5690-CD

• C Boch, M Reschke, F Igney, P Maier, P Müller, S Danklmaier, K Das, T Hofer, G Wollmann, W Rist Evaluating Antibody Pharmacokinetics as Prerequisite for Determining True Efficacy as Shown by Dual Targeting of PD-1 and CD96 Biomedicines, 2022-09-01;10(9):. 2022-09-01 [PMID: 36140247] (ELISA (Standard), Mouse)

Bioinformatics

• Gene Symbol: Cd96
• Uniprot:
  • AAH52865()