Recombinant Mouse CD27 Ligand/TNFSF7 Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse CD27 Ligand/TNFSF7 Protein Summary

Details of Functionality
Measured by its ability to stimulate mouse T cell proliferation in the presence of anti-CD3. The ED50 for this effect is 1.25-5.0 µg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse CD27 Ligand/TNFSF7 protein
MHHHHHHHHHH GGGSGGGSGGGS IEGR Mouse CD27 Ligand
(Gln47 - Pro195)
Accession # Q05A52
N-terminus C-terminus
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
Cd70
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
19.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
30 kDa, reducing conditions
Publications
Read Publications using
783-CL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CD27 Ligand/TNFSF7 Protein

  • CD27 Ligand
  • CD27-L
  • CD27LCD27 ligand
  • CD27LG
  • CD70 molecule
  • CD70
  • Ki-24 antigen
  • surface antigen CD70
  • TNFSF7
  • TNFSF7CD70 antigen
  • tumor necrosis factor (ligand) superfamily, member 7
  • Tumor necrosis factor ligand superfamily member 7

Background

CD27 ligand (CD27L), also named CD70, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7 (1, 2). Mouse CD27L cDNA encodes a 195 amino acid (aa) residue protein with a 23 aa N-terminal cytoplasmic domain, a 21 transmembrane domain and a 151 aa C-terminal extracellular domain. Mouse and human CD27L share approximated 56% aa sequence identity. By analogy to other TNFSF ligands, CD27L is expected to exist as non-covalent homotrimers. The expression of CD27L is highly regulated at the transcription and posttranslational level (3). CD27L cell surface expression is induced by antigen receptor activation in B cells and at low levels in mouse T cells. Although CD27L expression is not detected on human dendritic cells (DC), membrane expression on mature mouse DCs has been reported. CD27L expression is also present in the thymus medulla in both human and mouse. CD27L interacts with CD27, a member of the TNF receptor superfamily that is expressed on natural killer (NK) cells and subsets of T and B cells (2, 4, 5). Ligation of CD27 on T cells provides costimulatory signals that are required for T cell proliferation, clonal expansion and the promotion of effector T cell formation (2). Ligation of CD27 on mouse B cell has been shown to inhibit terminal differentiation of activated B cells into plasma cells and enhances commitment to memory B cell responses (5, 6). On NK cells, ligation of CD27 induces proliferation and IFN-gamma production (4).

  1. Hitzen, R.Q. et al. (1994) Immunol. Today 15:307.
  2. Croft, M. (2003) Nature Reviews Immunol. 3:609.
  3. Tesselaar, K. et al. (2003) J. Immunol. 169:33.
  4. Takeda, K. et al. (2000) J. Immunol. 164:1741.
  5. Kobata, T.S. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11249.
  6. Raman, V.S. et al. (2003) J. Immunol. 171:5876.

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Publications for CD27 Ligand/TNFSF7/CD70 (783-CL)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: Bioassay, In Vivo.


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(1)
In Vivo
(1)
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Bioinformatics

Gene Symbol Cd70
Uniprot