Recombinant Mouse CD229/SLAMF3 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CD229/SLAMF3 Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance anti-CD3-induced proliferation of mouse CD3⁺ T cells. The ED₅₀ for this effect is 0.5-2.5 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse CD229/SLAMF3 protein
  Lys48-Phe454, with a C-terminal 6-His tag
• Accession #: AAH95921
• N-terminal Sequence: Lys48
• Protein/Peptide Type: Recombinant Proteins
• Gene: Ly9
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-80 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CD229/SLAMF3 Protein, CF

• CD229 antigen
• CD229
• cell-surface molecule Ly-9
• hly9
• Ly9
• lymphocyte antigen 9Cell surface molecule Ly-9
• mLY9
• SLAMF3
• T-lymphocyte surface antigen Ly-9

Background

CD229, also known as Ly9 and SLAMF3, is a 120 kDa type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family (1). Mature mouse CD229 consists of a 406 aa extracellular domain (ECD) with two Ig‑like V‑set and two Ig‑like truncated C2‑set domains, a 21 aa transmembrane segment, and a 180 aa cytoplasmic domain with two immunoreceptor tyrosine‑based switch motifs ITSMs (2, 3). The ECD of mouse CD229 shares 59% and 73% aa sequence identity with human and rat CD229, respectively. Within the first two Ig‑like domains that are common to all SLAM proteins, mouse CD229 shares 22% ‑ 36% aa sequence identity with mouse 2B4, BLAME, CD2F‑10, CD84, CRACC, NTB‑A, and SLAM. CD229 is expressed on T, B, and NK cells, thymocytes, and monocytes (2 ‑ 7). Homophilic binding between CD229 molecules is mediated by the N‑terminal Ig‑like domain (8). Human and mouse CD229 exhibit cross‑species binding (8). Antigen stimulation of lymphocytes induces CD229 clustering to sites of T cell ‑ B cell contact (8). Y470 and Y606 in the cytoplasmic domain are required for association of CD229 with the adaptor proteins AP‑2 (μ2 chain) and GRB‑2, respectively, and both tyrosines are required for CD229 internalization (9, 10). The two ITSMs, which include Y558 and Y581, mediate phosphorylation‑dependent CD229 association with SAP and SHP‑2 (11). Antibody ligation of CD229 can inhibit T cell activation, but CD229 knockout mice show impaired T cell immune responses, suggesting a potential role for CD229 in T cell activation or co‑stimulation (7, 12).

1. Bhat, R. et al. (2006) J. Leukoc. Biol. 79:417.
2. de la Fuente, M.A. et al. (2001) Blood 97:3513.
3. Sandrin, M.S. et al. (1992) J. Immunol. 149:1636.
4. Romero, X. et al. (2004) Tissue Antigens 64:132.
5. Hogarth, P.M. et al. (1980) Immunogenetics 11:65.
6. Mathieson, B.J. et al. (1980) J. Immunol. 125:2127.
7. Sintes, J. et al. (2007) Tissue Antigens 70:355.
8. Romero, X. et al. (2005) J. Immunol. 174:7033.
9. Del Valle, J.M. et al. (2003) J. Biol. Chem. 278:17430.
10. Martin, M. et al. (2005) J. Immunol. 174:5977.
11. Sayos, J. et al. (2001) Blood 97:3867.
12. Graham, D.B. et al. (2006) J. Immunol. 176:291.

Bioinformatics

• Gene Symbol: Ly9
• Uniprot:
  • AAH95921()