Recombinant Mouse CCL6/C10 (Truncated, aa 42-116), CF

Recombinant Mouse CCL6/C10 (Catalog # 3210-C/CF) chemoattracts BaF3 mouse pro B cells transfected with human CCR1. The ED50 for this effect is 0.5-2 ng/mL.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CCL6/C10 (Truncated, aa 42-116), CF Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR1. The ED₅₀ for this effect is 0.5-2 ng/mL.
• Source: E. coli-derived mouse CCL6/C10 protein
  Gly42-Ala116
• Accession #: P27784
• N-terminal Sequence: Gly42
• Protein/Peptide Type: Recombinant Proteins
• Gene: Ccl6
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 8.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CCL6/C10 (Truncated, aa 42-116), CF

• C10
• c10;C-C motif chemokine 6;MRP;MRP-1;Scy;Scya6
• CCL6
• MRP-1
• SCYA6

Background

CCL6, also known as C10 and SCYA6, is an 11 kDa member of the MIP-I family of beta -chemokines. CCL6, along with CCL9, CCL15, and CCL23, belongs to the N6 subfamily of proteins that have an N-terminal extension relative to other beta -chemokines (1 - 4). The mouse CCL6 cDNA encodes a 116 amino acid (aa) precursor that includes a 21 aa signal sequence (5). Removal of an additional 20 aa at the N-terminal results in an 8 kDa protein (aa 42 - 116) and the transition from a weak CCR1 agonist to a dramatically more potent and efficacious macrophage chemoattractant (3, 6). The N-terminal region of CCL6 and other N6 subfamily chemokines is cleaved following incubation with synovial fluid from inflamed joints as well as with inflammation-associated proteases such as chymase, cathepsin G, and elastase (3). CCL6 is upregulated in activated macrophages at sites of inflammation (5, 7 - 10) and functions as a chemoattractant for additional macrophage influx (8, 9). The inflammatory cytokine IL-13 stimulates CCL6 production which then induces the release of CCL2, CCL3, and proteases involved in tissue repair (10). CCL6 expression is upregulated by the oncoprotein L-Myc but repressed by c-Myc. CCL6 induced by L-Myc promotes cellular transformation and the development of a tumorigenic phenotype (11). N-terminally truncated mouse CCL6 shares 77% and 64% aa sequence identity with rat CCL6 and mouse CCL9, respectively. A human orthologue for mouse CCL6 has not been identified. Conversely, CCL15 and CCL23 and other members of the N6 subfamily have been described in human but not in mouse.

1. Maurer, M. and E. von Stebut (2004) Int. J. Biochem. Cell Biol. 36:1882.
2. Trakatelli, C. et al. (2005) Int. J. Biol. Markers 20:197.
3. Berahovich, R.D. et al. (2005) J. Immunol. 174:7341.
4. Berger, M.S. et al. (1993) DNA Cell Biol. 12:839.
5. Orlofsky, A. et al. (1991) Cell Regul. 2:403.
6. Berger, M.S. et al. (1996) Cytokine 8:439.
7. Orlofsky, A. et al. (1994) J. Immunol. 152:5084.
8. Kaesler, S. et al. (2002) Cytokine 3:157.
9. LaFleur, A.M. et al. (2004) Mediators Inflamm. 13:349.
10. Ma, B. et al. (2004) J. Immunol. 172:1872.
11. Yi, F. et al. (2003) Cancer Res. 63:2923.

Publications for CCL6/C10 (3210-C/CF)(1)

Publication using 3210-C/CF

• C Hemmers, C Schulte, J Wollenhaup, DWL Wong, E Harlacher, S Orth-Alamp, BM Klinkhamme, SH Schirmer, M Böhm, N Marx, T Speer, P Boor, J Jankowski, H Noels Chemokine CCL9 Is Upregulated Early in Chronic Kidney Disease and Counteracts Kidney Inflammation and Fibrosis Biomedicines, 2022-02-10;10(2):. 2022-02-10 [PMID: 35203629] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Ccl6
• Uniprot:
  • P27784()