Recombinant Mouse CCL12/MCP-5 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CCL12/MCP-5 Protein, CF Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR2A. The ED₅₀ for this effect is 5‑30 ng/mL.
• Source: E. coli-derived mouse CCL12/MCP-5 protein
  Gly23-Gly104
• Accession #: Q62401.1
• N-terminal Sequence: Gly23
• Protein/Peptide Type: Recombinant Proteins
• Gene: Ccl12
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 9.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CCL12/MCP-5 Protein, CF

• C-C motif chemokine 12
• CCL12
• chemokine (C-C motif) ligand 12
• MCP5
• MCP-5
• Scya12

Background

CCL12 is a cloned mouse CC chemokine most closely related to human MCP-1 (66% amino acid sequence identity in the mature protein). Mouse CCL12 encodes a 104 amino acid residue precursor protein with a 22 aa residue predicted hydrophobic signal sequence that is cleaved to generate a 82 aa residue mature protein. CCL12 is expressed constitutively in the thymus and lymph nodes. Under inflammatory conditions, CCL12 expression is also induced in activated macrophages and mast cells. The gene for mouse MCP-1 has been mapped to the CC chemokine cluster on chromosome 11. Recombinant CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, CCL12 will also chemoattract eosinophils. CCL12 has been found to be a functional ligand for CCR-2 but not CCR-1, CCR-3 or CCR-5.

1. Jia, G.Q. et al. (1996) J. Exp. Med. 184:1939.
2. Sarafi, M.N. et al. (1997) J. Exp. Med. 185:99.

Publications for CCL12/MCP-5 (428-P5/CF)(3)

Publications using 428-P5/CF

• K Popiolek-B, A Ciechanows, K Ciapa?a, K Pawlik, M Oggioni, D Mercurio, MG De Simoni, J Mika --&gt;The CCL2/CCL7/CCL12/CCR2 pathway is substantially and persistently upregulated in mice after traumatic brain injury, and CCL2 modulates the complement system in microglia Mol Cell Probes, 2020-11-04;54(0):101671. 2020-11-04 [PMID: 33160071] (Cell Culture, Mouse)
• K Kwiatkowsk, K Popiolek-B, A Piotrowska, E Rojewska, K Ciapa?a, W Makuch, J Mika Chemokines CCL2 and CCL7, but not CCL12, play a significant role in the development of pain-related behavior and opioid-induced analgesia Cytokine, 2019-04-16;119(0):202-213. 2019-04-16 [PMID: 31003094] (In Vivo, Mouse)
• Ueno T, Kuse S, Saito F, Nitta T, Kakiuchi T, Hollander GA, Takahama Y The role of CCL21 in recruitment of T-precursor cells to fetal thymi. Blood, 2004-09-09;105(1):31-9. 2004-09-09 [PMID: 15358618] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Ccl12
• Uniprot:
  • Q62401.1()