Recombinant Mouse BMP-10 Protein

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse BMP-10 Protein Summary

• Details of Functionality: Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED₅₀ for this effect is 6-36 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse BMP-10 protein
  Asn314-Arg421
• Accession #: Q9R229
• N-terminal Sequence: Asn314
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 12.1 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 10 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse BMP-10 Protein

• BMP10
• BMP-10
• bone morphogenetic protein 10
• MGC126783

Background

Bone morphogenetic protein 10 (BMP-10), along with BMP-9, GDF-5, -6, and -7, belongs to a subgroup of TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors (1-3). Proteolytic removal of the propeptide from the 60 kDa proprotein yields a 12 kDa mature BMP-10 which forms disulfide-linked nonglycosylated homodimers (4, 5). In transfectants, BMP-10 can also be secreted as a proprotein which binds to matrix fibrillin (4, 6). Mature mouse BMP-10 shares 98% and 100% amino acid sequence identity with human and rat BMP-10, respectively, and 48%-64% with mouse BMP-9, GDF-5, -6, and -7. BMP-10 is critical for the proper development of the heart and first appears at the onset of trabeculation and chamber formation (7-9). Homozygous BMP-10 knockout mice die in utero due to arrested cardiac development (8). BMP-10 is required for maintaining expression of the cardiogenic transcription factors NKX2.5 and MEF2C in developing myocardium and promoting the Notch-dependent growth of embryonic cardiomyocytes (8, 10-12). NKX2.5 itself negatively regulates BMP-10 expression in cardiac myocytes (11). BMP-10 in the postnatal heart promotes increased cardiomyocyte and heart size and is upregulated in hypertrophied ventricles (9, 13). Mature BMP-10 accumulates within cardiomyocytes in association with the sarcomere protein Tcap (13). BMP-10 induces signaling through ALK-1, BMPR-IA, BMPR-IB, and BMPR-II in transfectants and non-cardiac cell lines (4, 5). Deletion of BMPR-IA or BMP-10 causes similar cardiac morphogenetic abnormalities (14). In dermal endothelial cells, BMP-10 induces migration, proliferation, and gene expression typically associated with ALK-1 (5).

1. Chen, D. et al. (2004) Growth Factors 22:233.
2. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.
3. Schneider, M.D. et al. (2003) Cytokine Growth Factor Rev. 14:1.
4. Mazerbourg, S. et al. (2005) J. Biol. Chem. 280:32122.
5. David, L. et al. (2007) Blood 109:1953.
6. Sengle, G. et al. (2008) J. Biol. Chem. 283:13874.
7. Neuhaus, H. et al. (1999) Mech. Dev. 80:181.
8. Chen, H. et al. (2004) Development 131:2219.
9. Chen, H. et al. (2006) J. Biol. Chem. 281:27481.
10. Srivastava, D. and Olson, E.N. (2000) Nature 407:221.
11. Pashmforoush, M. et al. (2004) Cell 117:373.
12. Grego-Bessa, J. et al. (2007) Dev. Cell 12:415.
13. Nakano, N. et al. (2007) Am. J. Heart Circ. Physiol. 293:H3396.
14. Gaussin, V. et al. (2002) Proc. Natl. Acad. Sci. 99:2878.

Publications for BMP-10 (6038-BP)(1)

Publication using 6038-BP

• John B. Lees-Shepard, Sean J. Stoessel, Julian T. Chandler, Keith Bouchard, Patricia Bento, Lorraine N. Apuzzo et al. An anti-ACVR1 antibody exacerbates heterotopic ossification by fibro-adipogenic progenitors in fibrodysplasia ossificans progressiva mice Journal of Clinical Investigation 2022-06-15 [PMID: 35503416] (Bioassay, Mouse)

Bioinformatics

• Uniprot:
  • Q9R229()