Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein, CF

When Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein (Catalog # 10336-BL) is immobilized at 5 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein that produces 50% of the optimal binding response is found to be approximately 4-20 μg/mL.

2 μg/lane of Recombinant Mouse BLAME/SLAMF8 Fc Chimera (Catalog # 10336-BL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 52-70 kDa and 100-140 kDa, respectively.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein (Catalog # 10336-BL) is immobilized at 5 μg/mL (100 µL/well), the concentration of Biotinylated Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein that produces 50% of the optimal binding response is found to be approximately 4‑20 µg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse BLAME/SLAMF8 protein
  

  Mouse BLAME/SLAMF8 (Val21-Asp231) Accession # NP_083360.2	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: NP_083360.2
• N-terminal Sequence: Val21
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 52-70 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse BLAME/SLAMF8 Fc Chimera Protein, CF

• B Lymphocyte Activator Macrophage Expressed
• BCM-Like Membrane Protein
• BLAME
• B-Lymphocyte Activator Macrophage Expressed
• CD353 Antigen
• CD353
• SBBI42
• SLAM Family Member 8
• SLAMF8

Background

BLAME (B-lymphocyte activator macrophage expressed), also known as SLAM family member 8, is a type I transmembrane protein that belongs to the CD2 subset of immunoglobulin superfamily cell receptors. CD2 family proteins function as adhesion molecules and modulators of immune responses (1, 2). It is expressed on dendritic cells and IFN-gamma stimulated monocytes. Over-expression of BLAME in bone marrow cells leads to an increase in the peritoneal B1b population of B lymphocytes (3, 4). Mature mouse BLAME consists of a 211 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 26 aa cytoplasmic domain. Within the ECD, mouse BLAME shares 79% and 95% aa sequence identity with human and rat BLAME, respectively.

1. McNerney, M.E. and V. Kumar (2006) Curr. Top. Microbiol. Immunol. 298:91.
2. Veillette, A. (2006) Nat. Rev. Immunol. 6:56.
3. Kingsbury, G.A. et al. (2001) J. Immunol. 166:5675.
4. Wang, G. et al. (2012) J. Immunol. 188:5829.

Bioinformatics

• Uniprot:
  • NP_083360.2()