Recombinant Mouse beta-Defensin 3 Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse beta-Defensin 3 Protein, CF Summary

• Details of Functionality: Measured by its anti-microbial activity against E. coli. Ganz, T. et al. (2003) Nat. Rev. Immunol. 3:710. The ED₅₀ for this effect is 4-20 μg/mL.
• Source: E. coli-derived mouse beta-Defensin 3 protein
  Lys23-Lys63
• Accession #: Q9WTL0
• N-terminal Sequence: Lys23
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 4.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 5 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in HCl.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 500 μg/mL in 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse beta-Defensin 3 Protein, CF

• BD14
• BD3
• BD-3
• beta-defensin 103
• betaDefensin 3
• beta-Defensin 3
• DEFB103
• DEFB103A
• DEFB103B
• DEFB3
• DEFB-3
• DEFB3DEFB103A
• Defensin, beta 103
• defensin, beta 103B
• Defensin-like protein
• HBD-3
• HBD3HBP3

Background

beta -defensin 3, also known as BD3 and DEFB-3, is a membrane-active cationic peptide that functions in inflammation and innate immune responses. There are at least 30 beta -defensins which are distinguished from alpha -defensins by the connectivity pattern of their three intramolecular disulfide bonds (1). The 41 aa mature mouse BD3 shares 38% and 59% aa sequence identity with human and rat BD3, respectively (2, 3). It shares 14% - 46% aa sequence identity with other mouse beta -defensins. BD3 is widely expressed among epithelial tissues, notably by keratinocytes and airway epithelial cells. It is up-regulated in response to proinflammatory cytokines, microbial and viral infections, and at the edges of skin wounds (2, 4 ‑ 6). BD3 induction in osteoarthritis chondrocytes promotes MMP1 and 13 production and inhibits TIMP1 and 2 expression (7). In vivo control of BD3 activity is accomplished in part through cleavage by cathepsins B, L, and S (8). BD3 displays strain specific microbicidal activity toward a broad spectrum of bacteria and yeast (2, 9). BD3 also induces monocyte migration, mast cell activation, and a mast cell-dependent increase in vascular permeability (4, 10). Disruption of the intramolecular disulfide bond pattern in BD3 abrogates its monocyte chemoattractant properties but not its antimicrobial properties (11, 12). BD3 inhibits viral infectivity by interacting directly with HIV-1 and its coreceptor CXCR4 (5, 13) and with HSV glycoprotein B and its receptor heparan sulfate (14), and by forming a protective coating on the surface of influenza virus target cells (15).

1. Dhople, V. et al. (2006) Biochim. Biophys. Acta. 1758:1499.
2. Bals, R. et al. (1999) Infect. Immun. 67:3542.
3. Schibli, D.J. et al. (2002) J. Biol. Chem. 277:8279.
4. Garcia, J.-R.C. et al. (2001) Cell Tissue Res. 306:257.
5. Quinones-Mateu, M.E. et al. (2003) AIDS 17:F39.
6. Sorensen, O.E. et al. (2006) J. Clin. Invest. 116:1878.
7. Varoga, D. et al. (2005) Arthritis Rheum. 52:1736.
8. Taggart, C.C. et al. (2003) J. Immunol. 171:931.
9. Joly, S. et al. (2004) J. Clin. Microbiol. 42:1024.
10. Chen, X. et al. (2007) Eur. J. Immunol. 37:434.
11. Kluver, E. et al. (2005) Biochemistry 44:9804.
12. Wu, Z. et al. (2003) Proc. Natl. Acad. Sci. 100:8880.
13. Feng, Z. et al. (2006) J. Immunol. 177:782.
14. Hazrati, E. et al. (2006) J. Immunol. 177:8658.
15. Leikina, E. et al. (2005) Nat. Immunol. 6:995.

Publications for beta-Defensin 3 (5987-BD)(1)

Publication using 5987-BD

• Rolin O, Muse S, Safi C, Elahi S, Gerdts V, Hittle L, Ernst R, Harvill E, Preston A Enzymatic modification of lipid A by ArnT protects Bordetella bronchiseptica against cationic peptides and is required for transmission. Infect Immun, 2013-10-14;82(2):491-9. 2013-10-14 [PMID: 24478065] (Bioassay, Bacteria - Bordetella bronchiseptica)

Bioinformatics

• Uniprot:
  • Q9WTL0()