Recombinant Mouse ASIP/Agouti Protein, CF

Recombinant Mouse ASIP/Agouti (Catalog # 9619-AG) inhibitsalpha-MSH induced eumelanin production in B16F1 mouse melanoma cells. The ED50for this effect is 0.8-4 μg/mL.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse ASIP/Agouti Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit alpha-MSH induced eumelanin production in B16F1 mouse melanoma cells. The ED₅₀ for this effect is 0.8-4 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse ASIP protein
  His23-Cys131
• Accession #: Q03288
• N-terminal Sequence: His23
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 12 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 19-24 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse ASIP/Agouti Protein, CF

• agouti (mouse)-signaling protein
• agouti signaling protein
• agouti signaling protein, nonagouti homolog (mouse)
• agouti signaling protein, nonagouti homolog
• Agouti switch protein
• agouti-signaling protein
• AGSW
• AGTI
• AGTIL
• AGTILMGC126092
• ASIP
• ASPAGSW
• MGC126093
• nonagouti homolog
• SHEP9

Background

Agouti, also known as Agouti Signaling Protein (ASIP), is a 131 amino peptide that regulates hair pigmentation in mice and adipocyte metabolism in humans (1). Mature mouse Agouti shares 78% and 94% amino acid sequence identity with human and rat Agouti, respectively (2, 3). Human Agouti is primarily expressed in adipose tissue, whereas its adipose expression in mice is only induced by mutations in the Agouti promoter (4, 5). Studies of human Agouti have shown that it is also expressed in the pancreas where it enhances the production of insulin by beta cells (6). In humans and mice, Agouti functions as a competitive antagonist at several melanocortin receptors. It blocks ACTH binding to MC1R and MSH binding to MC1R, MC3R, and MC4R (7-9). It blocks MSH induced effects on melanocytes including proliferation and melanin production (3, 7, 8).

1. Voisey, J. and A. van Daal (2002) Pigment Cell Res. 15:10.
2. Kwon, H.Y. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9760.
3. Wilson, B.D. et al. (1995) Hum. Mol. Genet. 4:223.
4. Mynatt, R.L. et al. (1997) Proc. Natl. Acad. Sci. USA 94:919.
5. Mynatt, R.L. and J.M. Stephens (2001) Am. J. Physiol. Cell Physiol. 280:C954.
6. Xue, B.Z. et al. (1999) Physiol. Genomics 1:11.
7. Lu, D. et al. (1994) Nature 371:799.
8. Suzuki, I. et al. (1997) J. Invest. Dermatol. 108:838.
9. Kiefer, L.L. et al. (1997) Biochemistry 36:2084.

Bioinformatics

• Uniprot:
  • Q03288()