Recombinant Human Wnt-10b Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human Wnt-10b Protein, CF Summary

Details of Functionality
Measured in a cell proliferation/survival assay using IEC‑18 rat small intestinal epithelial cells. The ED50 for this effect is 0.3-1.2 μg/mL
Source
Chinese Hamster Ovary cell line, CHO-derived human Wnt-10b protein
Met1-Lys389
Accession #
N-terminal Sequence
Asn29
Protein/Peptide Type
Recombinant Proteins
Gene
WNT10B
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
41 kDa, reducing conditions
Publications
Read Publications using
7196-WN/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Wnt-10b Protein, CF

  • protein Wnt-10b
  • Protein Wnt-12
  • SHFM6WNT-10B protein
  • wingless-type MMTV integration site family, member 10B
  • Wnt10b
  • Wnt-10b
  • WNT12
  • Wnt-12

Background

Wnt-10b (also known as Wnt-12) is a 42 ‑ 44 kDa member of the Wnt family of secreted, highly conserved, cysteine-rich glycoproteins that play important roles in vertebrate pattern formation, cell fate decision, axon guidance, and tumor formation (1 ‑ 3). Human Wnt-10b cDNA encodes a 389 amino acid (aa) precursor that contains a 28 aa signal sequence plus a 361 aa mature protein that contains two glycosylation sites, three potential phosphorylation sites, and a potential palmitoylation site (3, 4). Human Wnt-10b shares 97 ‑ 99% aa identity with mouse, rat, equine, porcine, and canine Wnt-10b. Wnt-10b plays a critical role in maintaining mesenchymal stem cells and determining whether they differentiate to adipocytes or osteoblasts (5 ‑ 7). Mouse Wnt-10b deletion produces age‑dependent loss of bone mass due to defective production of osteoblasts, while transgenic over‑expression increases postnatal osteoblast differentiation and inhibits adipocyte differentiation (5 ‑ 7). Ectopic expression of Wnt-10b in an obesity and diabetes‑prone background, such as the ob/ob mouse, inhibits obesity (8). In mouse skeletal muscle, Wnt-10b is expressed inversely with SREBP1c and increases insulin sensitivity (9). In humans, a missense polymorphism is responsible for a malformation of hands and feet, while a C256Y inactivating mutation is associated with severe early-onset obesity (10, 11). Wnt-10b is mainly produced by stem cells and pre-osteoblasts, but also by adult bone marrow CD8+ T lymphocytes stimulated with parathyroid hormone (12). In some hepatocellular carcinomas, Wnt-10b can inhibit cancer cell growth, but in others, it can act synergistically with FGFs to stimulate cell growth (13). Several Wnts, including Wnt-10b, are expressed in both normal and/or malignant colon tissues (14).

  1. Hardiman, G. et al. (1997) Cytogenet. Cell Genet. 77:278.
  2. Katoh, M. (2008) Curr. Drug Targets 9:565.
  3. Hausmann, G. et al. (2007) Nat. Rev. Mol. Cell Biol. 8:331.
  4. Swiss Prot Accession # O00744.
  5. Stevens, J.R. et al. (2010) J. Bone Miner. Res. 25:2138.
  6. Bennett, C.N. et al. (2007) J. Bone Miner. Res. 22:1924.
  7. Bennett, C.N. et al. (2005) Proc. Natl. Acad. Sci. USA 102:3324.
  8. Wright, W.S. et al. (2007) Diabetes 56:295.
  9. Abiola, M. et al. (2009) PLoS ONE 4:e8509.
  10. Ugur, S.A. and A. Tolun (2008) Hum. Mol. Genet. 17:2644.
  11. Christodoulides, C. et al. (2006) Diabetologia 49:678.
  12. Terauchi, M. et al. (2009) Cell Metab. 10:229.
  13. Yoshikawa, H. et al. (2007) Mol. Biol. Cell 18:4292.
  14. Holcombe, R.F. et al. (2002) J. Clin. Pathol: Mol. Pathol. 55:220.

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Publications for Wnt-10b (7196-WN/CF)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(6)
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Human
(6)
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Showing Publications 1 - 6 of 6.
Publications using 7196-WN/CF Applications Species
P Utaijaratr, K Vaeteewoot, T Tsunematsu, P Jamjantra, S Wongkham, C Pairojkul, N Khuntikeo, N Ishimaru, Y Sirivatana, A Pongpaibul, P Thuwajit, C Thuwajit, Y Kudo The microRNA-15a-PAI-2 axis in cholangiocarcinoma-associated fibroblasts promotes migration of cancer cells Mol. Cancer, 2018;17(1):10. 2018 [PMID: 29347950] (Bioassay, Human) Bioassay Human
J Jang, Y Jung, S Chae, SI Chung, SM Kim, Y Yoon WNT/?-catenin pathway modulates the TNF-?-induced inflammatory response in bronchial epithelial cells Biochem. Biophys. Res. Commun, 2017;0(0):. 2017 [PMID: 28137581] (Bioassay, Human) Bioassay Human
J Jang, Y Jung, Y Kim, EH Jho, Y Yoon LPS-induced inflammatory response is suppressed by Wnt inhibitors, Dickkopf-1 and LGK974 Sci Rep, 2017;7(0):41612. 2017 [PMID: 28128299] (Bioassay, Human) Bioassay Human
Calderon-Gierszal E, Prins G Directed Differentiation of Human Embryonic Stem Cells into Prostate Organoids In Vitro and its Perturbation by Low-Dose Bisphenol A Exposure. PLoS ONE, 2015;10(7):e0133238. 2015 [PMID: 26222054] (Bioassay, Human) Bioassay Human
Kilander M, Dahlstrom J, Schulte G Assessment of Frizzled 6 membrane mobility by FRAP supports G protein coupling and reveals WNT-Frizzled selectivity. Cell Signal, 2014;26(9):1943-9. 2014 [PMID: 24873871] (Bioassay, Human) Bioassay Human
Thrasivoulou C, Millar M, Ahmed A Activation of intracellular calcium by multiple Wnt ligands and translocation of beta-catenin into the nucleus: a convergent model of Wnt/Ca2+ and Wnt/beta-catenin pathways. J Biol Chem, 2013;288(50):35651-9. 2013 [PMID: 24158438] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol WNT10B
Uniprot