Recombinant Human UNC5H4 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human UNC5H4 Fc Chimera Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Mouse myeloma cell line, NS0-derived human UNC5H4 protein
  

  Human UNC5H4 (Gly15 - Ser373) Accession # BAB83663	IEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #: BAB83663
• N-terminal Sequence: Gly15 & Phe19
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: UNC5D
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 66 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 95-100 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,939,271, 6,277,585, and other U.S. and international patents pending.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human UNC5H4 Fc Chimera Protein, CF

• FLJ16019
• KIAA1777Unc5h4
• netrin receptor UNC5D
• netrin receptor Unc5h4
• PRO34692
• Protein unc-5 homolog 4
• Protein unc-5 homolog D
• unc-5 homolog 4
• unc-5 homolog D (C. elegans)
• UNC5D
• UNC5H4

Background

Caenorhabditis elegans UNC5 (UNC = behaviorally uncoordinated) and its mammalian homologues, UNC5H1-4, UNC5A-D, and rostral cerebellar malformation (RCM), are transmembrane proteins belonging to the immunoglobulin (Ig) superfamily. All UNC5 family members have two Ig and two thrombospondin type 1 domains in their extracellular regions, as well as a conserved ZU-5 domain, a DCC (Deleted in Colorectal Cancer)-binding domain (DB) and a C-terminal death domain (DD) in their cytoplasmic regions (1, 2). Human UNC5H4 cDNA encodes a 948 amino acid (aa) residues type I membrane protein with a putative 14 aa signal peptide and 359 aa extracellular domain. The extracellular domain of human UNC5H4 shares approximately 97% and 66% aa sequence identity with mouse UNC5H4 and human UNC5H3, respectively.

UNC5 family proteins are receptors for the netrin/UNC6 family of secreted axon guidance cues that are laminin-related proteins. Netrin family proteins can act as a chemoattractant for some axons and as a chemorepellent for others. Besides UNC5, netrin family proteins also bind to the DCC family of type I transmembrane receptors and to adenosine A2b receptor, a G protein-coupled seven‑transmembrane receptor belonging to the adenosine receptor family (3, 4). In vitro, netrin binding to DCC family receptors in the absence of UNC5 is associated with axon attraction. However, the DCC‑mediated attraction to netrin is converted to repulsion by binding of UNC5 to the DCC-netrin complex. In vivo, the mechanisms of netrin-dependent axon attraction and repulsion are more complex and may include UNC5-mediated repulsion that is independent of DCC (1, 5). Besides their roles in axon guidance and neuronal migration, the UNC5 and DCC families also act as dependence receptors and exert pro-apoptotic effects in the absence of netrin (6).

1. Hong, K. et al. (1999) Cell 97:927.
2. Leonardo, E.D. et al. (1997) Nature 386:833.
3. Culotti, J.B. and D.C. Merz (1998) Curr. Opin. Cell Biol. 10:609.
4. Corset, V. (2000) Nature 407:747.
5. Merz, D.C. (2001) Genetics 158:1071.
6. Llambi, F. et al. (2001) EMBO Journal 20:2715.

Publications for UNC5H4/UNC5D (1429-UN)(1)

Publication using 1429-UN

• VA Jackson, S Mehmood, M Chavent, P Roversi, M Carrasquer, D Del Toro, G Seyit-Brem, FM Ranaivoson, D Comoletti, MS Sansom, CV Robinson, R Klein, E Seiradake Super-complexes of adhesion GPCRs and neural guidance receptors Nat Commun, 2016-04-19;7(0):11184. 2016-04-19 [PMID: 27091502] (Bioassay, Human)

Bioinformatics

• Gene Symbol: UNC5D
• Uniprot:
  • BAB83663()