Recombinant Human UNC5B Protein Fc Chimera, CF


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Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human UNC5B Protein Fc Chimera, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human UNC5H2/UNC5B Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse Netrin-1 (Catalog # 1109-N1) that produces 50% optimal binding response is approximately 7-42 ng/mL.
Chinese Hamster Ovary cell line, CHO-derived human UNC5H2/UNC5B protein
Human UNC5H2/UNC5B
Accession # Q8IZJ1
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE with silver staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
66 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
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8869-UN in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>95%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 400 μg/mL in PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human UNC5B Protein Fc Chimera, CF

  • p53RDL1
  • p53-regulated receptor for death and life protein 1
  • Protein unc-5 homolog 2
  • Protein unc-5 homolog B
  • transmembrane receptor Unc5H2
  • unc5 (C.elegans homolog) b
  • unc-5 homolog 2
  • unc-5 homolog B (C. elegans)
  • UNC5B
  • UNC5H2
  • UNC5H2netrin receptor UNC5B


Netrin Receptor UNC5B is one of four related receptors that comprise the UNC5 family and serve as receptors for the axon guidance molecule Netrin-1. UNC5B is a type I transmembrane protein that contains two Ig-like and two thrombospondin domains in its extracellular domain (ECD), and a ZU5 domain, a DCC-binding domain, and a death domain in its intracellular region (1, 2). It is widely expressed in various embryonic and adult tissues including neural, hematopoietic, and immune tissues (1, 3, 4). Human UNC5B has a predicted molecular weight of approximately 104 kDa. Its ECD shares approximately 92% amino acid sequence identity within shared regions in the mouse and rat orthologs, which are termed UNC5H2. UNC5B regulates axon guidance by facilitating Netrin-1-mediated axon repulsion of neuronal growth cones (1). It complexes with the attractive Netrin receptor DCC and switches its response to Netrin-1 from attraction to repulsion (5). UNC5B can also induce growth cone collapse via its associations with the RGM-A receptor Neogenin and the soluble ECD of FLRT3 (2, 6). Outside of the nervous system, UNC5B facilitates Netrin-1-mediated inhibition of capillary branching and leukocyte migration, as well as Netrin-1-mediated promotion of osteoclast differentiation (7-10). UNC5B also functions as a dependence receptor. In the absence of Netrin-1, UNC5B mediates p53-dependent apoptosis via activation of DAPK (11, 12). UNC5B is believed to play a role in tumorgenesis. It has been shown to inhibit tumor cell growth and invasion, and its expression appears to be down-regulated in multiple cancers including colorectal, breast, ovary, uterus, stomach, lung, and kidney (13, 14). Additionally, UNC5B is thought to play a critical role in the development of atherosclerosis and the survival of renal tubular epithelial cells during acute kidney injury (15, 16).
  1. Leonardo, E.D. et al. (1997) Nature 386:833.
  2. Hata, K. et al. (2009) J. Cell Biol. 184:737.
  3. Engelkamp, D. (2002) Mech. Dev. 118:191.
  4. Manitt, C. et al. (2004) J. Neurosci. Res. 77:690.
  5. Hong, K. et al. (1999) Cell 97:927.
  6. Yamagishi, S. et al. (2011) EMBO J. 30:2920.
  7. Lu, X. et al. (2004) Nature 432:179.
  8. Ly, N.P. et al. (2005) Proc. Natl. Acad. Sci. USA 102:14729.
  9. Larrivée, B. et al. (2007) Genes Dev. 21:2433.
  10. Mediero, A. et al. (2015) J. Bone Miner. Res. 30:837.
  11. Tanikawa, C. et al. (2003) Nat. Cell Biol. 5:216.
  12. Llambi, F. et al. (2005) EMBO J. 24:1192.
  13. Thiébault, K. et al. (2003) Proc. Natl. Acad. Sci. USA 100:4173.
  14. Arakawa, H. (2004) Nat. Rev. Cancer 4:978.
  15. Oksala, N. et al. (2013) Circ. Cardiovasc. Genet. 6:579.
  16. Ranganathan, P. et al. (2014) J. Am. Soc. Nephrol. 25:239.

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Gene Symbol UNC5B