Recombinant Human UNC5B Protein Fc Chimera, CF Summary
Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human UNC5H2/UNC5B Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of
Recombinant Mouse Netrin-1 (Catalog # 1109-N1)
that produces 50% optimal binding response is approximately 7-42 ng/mL.
Chinese Hamster Ovary cell line, CHO-derived human UNC5H2/UNC5B protein
Human UNC5H2/UNC5B (Gly27-Tyr377) Accession # Q8IZJ1
<0.10 EU per 1 μg of the protein by the LAL method.
66 kDa (monomer). Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>95%, by SDS-PAGE with silver staining.
Reconstitute at 400 μg/mL in PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human UNC5B Protein Fc Chimera, CF
p53-regulated receptor for death and life protein 1
Protein unc-5 homolog 2
Protein unc-5 homolog B
transmembrane receptor Unc5H2
unc5 (C.elegans homolog) b
unc-5 homolog 2
unc-5 homolog B (C. elegans)
UNC5H2netrin receptor UNC5B
Netrin Receptor UNC5B is one of four related receptors that comprise the UNC5 family and serve as receptors for the axon guidance molecule Netrin-1. UNC5B is a type I transmembrane protein that contains two Ig-like and two thrombospondin domains in its extracellular domain (ECD), and a ZU5 domain, a DCC-binding domain, and a death domain in its intracellular region (1, 2). It is widely expressed in various embryonic and adult tissues including neural, hematopoietic, and immune tissues (1, 3, 4). Human UNC5B has a predicted molecular weight of approximately 104 kDa. Its ECD shares approximately 92% amino acid sequence identity within shared regions in the mouse and rat orthologs, which are termed UNC5H2. UNC5B regulates axon guidance by facilitating Netrin-1-mediated axon repulsion of neuronal growth cones (1). It complexes with the attractive Netrin receptor DCC and switches its response to Netrin-1 from attraction to repulsion (5). UNC5B can also induce growth cone collapse via its associations with the RGM-A receptor Neogenin and the soluble ECD of FLRT3 (2, 6). Outside of the nervous system, UNC5B facilitates Netrin-1-mediated inhibition of capillary branching and leukocyte migration, as well as Netrin-1-mediated promotion of osteoclast differentiation (7-10). UNC5B also functions as a dependence receptor. In the absence of Netrin-1, UNC5B mediates p53-dependent apoptosis via activation of DAPK (11, 12). UNC5B is believed to play a role in tumorgenesis. It has been shown to inhibit tumor cell growth and invasion, and its expression appears to be down-regulated in multiple cancers including colorectal, breast, ovary, uterus, stomach, lung, and kidney (13, 14). Additionally, UNC5B is thought to play a critical role in the development of atherosclerosis and the survival of renal tubular epithelial cells during acute kidney injury (15, 16).
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