Recombinant Human UNC5B Protein Fc Chimera, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human UNC5B Protein Fc Chimera, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human UNC5H2/UNC5B Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse Netrin-1 (Catalog # 1109-N1) that produces 50% optimal binding response is approximately 7-42 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human UNC5H2/UNC5B protein
  

  Human UNC5H2/UNC5B (Gly27-Tyr377) Accession # Q8IZJ1	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q8IZJ1
• N-terminal Sequence: Gly27
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: UNC5B
• Purity: >95%, by SDS-PAGE with silver staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 66 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE with silver staining.
• Reconstitution Instructions: Reconstitute at 400 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human UNC5B Protein Fc Chimera, CF

• p53RDL1
• p53-regulated receptor for death and life protein 1
• Protein unc-5 homolog 2
• Protein unc-5 homolog B
• transmembrane receptor Unc5H2
• unc5 (C.elegans homolog) b
• unc-5 homolog 2
• unc-5 homolog B (C. elegans)
• UNC5B
• UNC5H2
• UNC5H2netrin receptor UNC5B

Background

Netrin Receptor UNC5B is one of four related receptors that comprise the UNC5 family and serve as receptors for the axon guidance molecule Netrin-1. UNC5B is a type I transmembrane protein that contains two Ig-like and two thrombospondin domains in its extracellular domain (ECD), and a ZU5 domain, a DCC-binding domain, and a death domain in its intracellular region (1, 2). It is widely expressed in various embryonic and adult tissues including neural, hematopoietic, and immune tissues (1, 3, 4). Human UNC5B has a predicted molecular weight of approximately 104 kDa. Its ECD shares approximately 92% amino acid sequence identity within shared regions in the mouse and rat orthologs, which are termed UNC5H2. UNC5B regulates axon guidance by facilitating Netrin-1-mediated axon repulsion of neuronal growth cones (1). It complexes with the attractive Netrin receptor DCC and switches its response to Netrin-1 from attraction to repulsion (5). UNC5B can also induce growth cone collapse via its associations with the RGM-A receptor Neogenin and the soluble ECD of FLRT3 (2, 6). Outside of the nervous system, UNC5B facilitates Netrin-1-mediated inhibition of capillary branching and leukocyte migration, as well as Netrin-1-mediated promotion of osteoclast differentiation (7-10). UNC5B also functions as a dependence receptor. In the absence of Netrin-1, UNC5B mediates p53-dependent apoptosis via activation of DAPK (11, 12). UNC5B is believed to play a role in tumorgenesis. It has been shown to inhibit tumor cell growth and invasion, and its expression appears to be down-regulated in multiple cancers including colorectal, breast, ovary, uterus, stomach, lung, and kidney (13, 14). Additionally, UNC5B is thought to play a critical role in the development of atherosclerosis and the survival of renal tubular epithelial cells during acute kidney injury (15, 16).

1. Leonardo, E.D. et al. (1997) Nature 386:833.
2. Hata, K. et al. (2009) J. Cell Biol. 184:737.
3. Engelkamp, D. (2002) Mech. Dev. 118:191.
4. Manitt, C. et al. (2004) J. Neurosci. Res. 77:690.
5. Hong, K. et al. (1999) Cell 97:927.
6. Yamagishi, S. et al. (2011) EMBO J. 30:2920.
7. Lu, X. et al. (2004) Nature 432:179.
8. Ly, N.P. et al. (2005) Proc. Natl. Acad. Sci. USA 102:14729.
9. Larrivée, B. et al. (2007) Genes Dev. 21:2433.
10. Mediero, A. et al. (2015) J. Bone Miner. Res. 30:837.
11. Tanikawa, C. et al. (2003) Nat. Cell Biol. 5:216.
12. Llambi, F. et al. (2005) EMBO J. 24:1192.
13. Thiébault, K. et al. (2003) Proc. Natl. Acad. Sci. USA 100:4173.
14. Arakawa, H. (2004) Nat. Rev. Cancer 4:978.
15. Oksala, N. et al. (2013) Circ. Cardiovasc. Genet. 6:579.
16. Ranganathan, P. et al. (2014) J. Am. Soc. Nephrol. 25:239.

Publications for UNC5H2/UNC5B (8869-UN)(3)

Publications using 8869-UN

• K Boyé, LH Geraldo, J Furtado, L Pibouin-Fr, M Poulet, D Kim, B Nelson, Y Xu, L Jacob, N Maissa, D Agalliu, L Claesson-W, SL Ackerman, A Eichmann Endothelial Unc5B controls blood-brain barrier integrity Nature Communications, 2022-03-04;13(1):1169. 2022-03-04 [PMID: 35246514] (Bioassay, Human)
• M Yamada, Y Fujita, Y Hayano, H Hayakawa, K Baba, H Mochizuki, T Yamashita Increased expression of fibronectin leucine-rich transmembrane protein 3 in the dorsal root ganglion induces neuropathic pain in rats J. Neurosci., 2019-07-25;0(0):. 2019-07-25 [PMID: 31346030] (ELISA Capture, Human)
• I Tai-Nagara, Y Yoshikawa, N Numata, T Ando, K Okabe, Y Sugiura, M Ieda, N Takakura, O Nakagawa, B Zhou, K Okabayashi, M Suematsu, Y Kitagawa, M Bastmeyer, K Sato, R Klein, S Navankasat, DY Li, S Yamagishi, Y Kubota Placental labyrinth formation in mice requires endothelial FLRT2-UNC5B signaling Development, 2017-06-02;0(0):. 2017-06-02 [PMID: 28576770] (Bioassay, Human)

Bioinformatics

• Gene Symbol: UNC5B
• Uniprot:
  • Q8IZJ1()