Recombinant Human TSLPR Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human TSLPR Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit TSLP-dependent proliferation of BaF3 mouse pro‑B cells co-transfected with human IL-7 R alpha and human TSLP R. The ED₅₀ for this effect is 10-30 µg/mL in the presence of 0.2 ng/mL of recombinant human TSLP.
• Source: Mouse myeloma cell line, NS0-derived human TSLPR protein
  

  Human TSLP R (Gly25-Lys231) Accession #Q9HC73	DIEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q9HC73
• N-terminal Sequence: Gly25
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: CRLF2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 50.6 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 65-75 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TSLPR Fc Chimera Protein, CF

• CRL2
• CRL2cytokine receptor CRL2 precusor
• CRLF2
• CRLF2Y
• CRLM-2
• Cytokine receptor-like 2
• cytokine receptor-like factor 2
• ILXR
• IL-XR
• P2RY8/CRLF2 fusion
• PCOR1
• Thymic stromal lymphopoietin protein receptor
• thymic stromal lymphopoietin receptor
• thymic stromal-derived lymphopoietin receptor
• TSLP R
• TSLP receptor
• TSLPR

Background

TSLPR, also named Delta (1) and CRLM-2 (2) (cytokine receptor-like module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain. It was subsequently identified to be a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLPR (3). The human TSLPR cDNA encodes a 371 amino acid (aa) residue type 1 membrane protein with a 22 aa residue signal peptide, a 210 aa residue extracellular domain, a 20 aa residue transmembrane domain, and a 119 aa residue cytoplasmic domain (4, 5). The extracellular region contains two fibronectin type III-like domains and a WSXWS-like motif. The cytoplasmic domain contains a membrane-proximal box 1 motif that is known to be important for association with JAKs (4). Human TSLPR displays 39% identity to mouse TSLPR and 24% identity to the common gamma receptor (4). An alternatively spliced mRNA variant encoding a soluble TSLPR has also been reported in mouse (2). TSLPR expression is ubiquitous in the immune and hematopoietic cells, but is up-regulated in Th2-skewed cells. Cells expressing TSLPR alone bind TSLP with low affinity. Co-expression of TSLPR and IL-7 R alpha is required for high-affinity TSLP binding and signal transduction (3-6). The TSLPR and IL-7 R alpha are co-expressed primarily on monocytes and dendritic cells and at lower levels in lymphoid cells. TSLP has been shown to induce the release of T cell-attracting chemokines from monocytes and enhance the maturation of CD11c⁺ dendritic cells (5).

1. Fujio, K. et al. (2000) Blood 95:2204.
2. Hiroyama, T. et al. (2000) Biochem. Biophys. Res. Commun. 272:224.
3. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
4. Tonozuka, Y. et al. (2001) Cytogenet. Cell Genet. 93:23.
5. Reche, P.A. et al. (2001) J. Immunol. 167:336.
6. Pandey, A. et al. (2000) Nat. Immunol. 1:59.

Publications for TSLPR/CRLF2 (981-TR)(1)

Publication using 981-TR

• Ohba T, Haro H, Ando T, Koyama K, Hatsushika K, Suenaga F, Ohnuma Y, Nakamura Y, Katoh R, Ogawa H, Hamada Y, Nakao A A potential role of thymic stromal lymphopoietin in the recruitment of macrophages to mouse intervertebral disc cells via monocyte chemotactic protein 1 induction: implications for herniated discs. Arthritis Rheum., 2008-11-01;58(11):3510-9. 2008-11-01 [PMID: 18975342] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: CRLF2
• Uniprot:
  • Q9HC73()