Recombinant Human TREML2/TLT-2 Fc Chimera Protein, CF

Images

 
There are currently no images for TREML2/TLT-2 (3259-TL).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human TREML2/TLT-2 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. Human PBMC cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with anti-CD3 and recombinant human TREML2/Fc Chimera.
The ED50 for this effect is 0.45-1.8 μg/mL.
Optimal dilutions should be determined by each laboratory for each applications.
Source
Mouse myeloma cell line, NS0-derived human TREML2/TLT-2 protein
Human TREML2
(Gly78 - Ser327)
Accession # CAI19909
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Gly78
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
TREML2
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
53.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
75-100 kDa, reducing conditions
Publications
Read Publications using
3259-TL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TREML2/TLT-2 Fc Chimera Protein, CF

  • C6orf76
  • dJ238O23.1
  • FLJ13693
  • MGC149715
  • MGC149716
  • TLT2
  • TLT-2
  • TREML2
  • trem-like transcript 2 protein
  • triggering receptor expressed on myeloid cells-like 2
  • triggering receptor expressed on myeloid cells-like protein 2
  • UNQ6268/PRO20473

Background

Trem-like transcript 2 protein (TLT-2; also TREML2) is a 33 kDa (unglycosylated) type I transmembrane cell surface receptor and member of the Trem family of receptor proteins. Human TLT-2 is synthesized as a 321 amino acid (aa) precursor that contains an 18 aa signal sequence, a 250 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 32 aa cytoplasmic tail (SwissProt # Q5T2D2). Within the ECD are an Ig-like V-type domain (aa 20 ‑ 121) and one potential site of N-linked glycosylation. Mature human TLT-2 shares 49% aa sequence identity with mature mouse TLT-2. Cell surface expression of TLT-2 is seen for most of the B and myeloid cell lines (1 ‑ 2). TLT-2 is also expressed constitutively on CD8+ T cells and induced on CD4+ T cells after activation (2). TLT-2 plays a role in the innate and adaptive immune responses. In 2008, Hashiguchi et al. discovered that the B7 family member B7-H3, which also plays important roles in immune responses, specifically bound to TLT-2. According to their research, stimulation with B7-H3 transfectants preferentially up-regulated the proliferation and IFN-gamma production on CD8+ T cells (2). Also, transduction of TLT-2 into T cells resulted in enhanced IL-2 and IFN-gamma production via interactions with B7-H3, and blockade of the B7‑H3:TLT‑2 pathway with mAb against B7-H3 or TLT-2 efficiently inhibited contact hypersensitivity responses (2). In 2009, however, Leitner et al. extensively analyzed interaction of B7-H3 with TLT-2 and found no evidence for such an interaction (3).
  1. King, R.G. et al. (2006) J. Immunol. 176:6012.
  2. Hashiguchi, M. et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105:10495.
  3. Leitner, J. et al. (2009) Eur. J. Immunol. 39:1754.

Customers Who Viewed This Item Also Viewed...

MAB140
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, IHC, Neut
7268-CT
Species: Hu
Applications: BA
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
AF1027
Species: Hu
Applications: CyTOF-ready, Flow, IHC, KO, Simple Western, WB
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
202-IL
Species: Hu
Applications: BA
AF1729
Species: Mu
Applications: ELISA, ICC, KO, WB
DY417
Species: Mu
Applications: ELISA
DTRM10C
Species: Hu
Applications: ELISA
AF2154
Species: Hu, Mu
Applications: CyTOF-ready, Flow, WB
NB100-56508
Species: Av, Hu, Mu, Rt
Applications: CyTOF-ready, Flow-CS, Flow, IHC, IHC-P, WB
NBP1-84234
Species: Hu
Applications: IHC, IHC-P, WB
2249-NK
Species: Hu
Applications: BA
AF5117
Species: Hu
Applications: IHC, WB
H00728695-B01P
Species: Hu
Applications: ICC/IF, IP, WB
NBP1-88927
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-43435
Species: Hu, Mu
Applications: Flow, WB
NBP1-49045
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
3259-TL
Species: Hu
Applications: Bioactivity

Publications for TREML2/TLT-2 (3259-TL)(3)

Reviews for TREML2/TLT-2 (3259-TL) (0)

There are no reviews for TREML2/TLT-2 (3259-TL). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for TREML2/TLT-2 (3259-TL) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional TREML2/TLT-2 Products

Bioinformatics Tool for TREML2/TLT-2 (3259-TL)

Discover related pathways, diseases and genes to TREML2/TLT-2 (3259-TL). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TREML2/TLT-2 (3259-TL)

Discover more about diseases related to TREML2/TLT-2 (3259-TL).
 

Pathways for TREML2/TLT-2 (3259-TL)

View related products by pathway.

Blogs on TREML2/TLT-2

There are no specific blogs for TREML2/TLT-2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human TREML2/TLT-2 Fc Chimera Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol TREML2
Uniprot