Recombinant Human Thrombopoietin (NS0-expressed) Protein

Images

 
There are currently no images for Thrombopoietin/THPO (288-TPN).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human Thrombopoietin (NS0-expressed) Protein Summary

Additional Information
A new economical version of TPO made in E. coli is now available.
Details of Functionality
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The ED50 for this effect is 5-15 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human Thrombopoietin/Tpo protein
Ser22-Gly353
Accession #
N-terminal Sequence
Ser22
Protein/Peptide Type
Recombinant Proteins
Gene
THPO
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.1 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
80-90 kDa, reducing conditions
Publications
Read Publications using
288-TPN in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Thrombopoietin (NS0-expressed) Protein

  • Megakaryocyte colony-stimulating factor
  • Megakaryocyte growth and development factor
  • megakaryocyte stimulating factor
  • MGDF
  • MGDFC-mpl ligand
  • MKCSF
  • MK-CSF
  • ML
  • MPL ligand
  • MPLLG
  • MPLLGMGC163194
  • Myeloproliferative leukemia virus oncogene ligand
  • THCYT1
  • THPO
  • thrombopoietin nirs variant 1
  • Thrombopoietin
  • Tpo
  • TPOMKCSF

Background

Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c-mpl. Defects in the Tpo-Tpo R signaling pathway are associated with a variety of platelet disorders (1 - 3). The 353 amino acid (aa) human Tpo precursor is cleaved to yield the 332 aa mature protein. Mature human Tpo shares approximately 70% aa sequence homology with mouse and rat Tpo. It is an 80 - 85 kDa protein that consists of an N-terminal domain with homology to Erythropoietin (Epo) and a C-terminal domain that contains multiple N-linked and O-linked glycosylation sites (4, 5). Tissue specific alternate splicing of human Tpo generates multiple isoforms with internal deletions, insertions, and/or C-terminal substitutions (6). Tpo promotes the differentiation, proliferation, and maturation of MK and their progenitors (4, 5, 7). Several other cytokines can promote these functions as well but only in cooperation with Tpo (8, 9). Notably, IL-3 independently induces MK development, although its effects are restricted to early in the MK lineage (8, 9). Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation (10 - 13). It is cleaved by platelet-derived thrombin following Arg191 within the C-terminal domain and subsequently at other sites upon extended digestion (14). Full length Tpo and shorter forms circulate in the plasma (4, 5). The C terminal domain is not required for binding to Tpo R or inducing MK growth and differentiation (5). Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxia-sensitized neurons and inhibits neuronal differentiation by blocking NGF induced signaling (15, 16).

  1. Deutsch, V.R. and A. Tomer (2006) Br. J. Haematol. 134:453.
  2. Kaushansky, K. (2005) J. Clin. Invest. 115:3339.
  3. Li, J. et al. (1999) Br. J. Haematol. 106:345.
  4. Bartley, T.D. et al. (1994) Cell 77:1117.
  5. de Sauvage, F.J. et al. (1994) Nature 369:533.
  6. Marcucci, R. and M. Romano (2008) Biochim. Biophys. Acta 1782:427.
  7. Kaushansky, K. et al. (1994) Nature 369:568.
  8. Kaushansky, K. et al. (1995) Proc. Natl. Acad. Sci. 92:3234.
  9. Broudy, V.C. et al. (1995) Blood 85:1719.
  10. Lok, S.I. et al. (1994) Nature 369:565.
  11. Chen, J. et al. (1995) Blood 86:4054.
  12. Oda, A. et al. (1996) Blood 87:4664.
  13. Van Os, E. et al. (2003) Br. J. Haematol. 121:482.
  14. Kato, T. et al. (1997) Proc. Natl. Acad. Sci. 94:4669.
  15. Ehrenreich, H. et al. (2005) Proc. Natl. Acad. Sci. 102:862.
  16. Samoylenko, A. et al. (2008) Cell. Signal. 20:154.

Customers Who Viewed This Item Also Viewed...

AF1016
Species: Hu
Applications: WB, Neut
7270-IL/CF
Species: Hu
NB600-1071
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-34294
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P
7954-GM/CF
Species: Hu
NBP2-67429
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
7954-GM/CF
Species: Hu
NBP2-13967
Species: Hu
Applications: IHC, IHC-P
NBP2-59376
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IP
AF8516
Species: Hu
Applications: WB, Simple Western, IP, ICC
AF-258-PB
Species: Hu
Applications: WB, Simple Western, IHC
NBP1-84581
Species: Hu
Applications: WB, Simple Western, IHC, IHC-P

Publications for Thrombopoietin/THPO (288-TPN)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(5)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using 288-TPN Applications Species
Niwa A, Heike T, Umeda K A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors. PLoS ONE, 2011;6(7):e22261. 2011 [PMID: 21818303] (Bioassay, Human) Bioassay Human
Uchida N, Hsieh MM, Hayakawa J, Madison C, Washington KN, Tisdale JF Optimal conditions for lentiviral transduction of engrafting human CD34+ cells. Gene Ther., 2011;18(11):1078-86. 2011 [PMID: 21544097] (Bioassay, Human) Bioassay Human
Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E A multiplex immunoassay for human adipokine profiling. Clin. Chem., 2010;56(0):1320. 2010 [PMID: 20530731]
Kobune M, Kawano Y, Takahashi S, Takada K, Murase K, Iyama S, Sato T, Takimoto R, Niitsu Y, Kato J Interaction with human stromal cells enhances CXCR4 expression and engraftment of cord blood Lin(-)CD34(-) cells. Exp. Hematol., 2008;36(9):1121-31. 2008 [PMID: 18562079] (Bioassay, Human) Bioassay Human
Rajesh&lt;/LastName&gt;&lt;ForeNam D&lt;/Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells. Stem Cells, 2007;25(2):490-9. 2007 [PMID: 17284653] (Bioassay, Mouse) Bioassay Mouse
Manz MG, Miyamoto T, Akashi K, Weissman IL Prospective isolation of human clonogenic common myeloid progenitors. Proc. Natl. Acad. Sci. U.S.A., 2002;99(18):11872-7. 2002 [PMID: 12193648] (Bioassay, Human) Bioassay Human

Reviews for Thrombopoietin/THPO (288-TPN) (0)

There are no reviews for Thrombopoietin/THPO (288-TPN). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Thrombopoietin/THPO (288-TPN) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional Thrombopoietin/THPO Products

Bioinformatics Tool for Thrombopoietin/THPO (288-TPN)

Discover related pathways, diseases and genes to Thrombopoietin/THPO (288-TPN). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Thrombopoietin/THPO (288-TPN)

Discover more about diseases related to Thrombopoietin/THPO (288-TPN).
 

Pathways for Thrombopoietin/THPO (288-TPN)

View related products by pathway.

PTMs for Thrombopoietin/THPO (288-TPN)

Learn more about PTMs related to Thrombopoietin/THPO (288-TPN).

Blogs on Thrombopoietin/THPO

There are no specific blogs for Thrombopoietin/THPO, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Thrombopoietin (NS0-expressed) Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol THPO
Uniprot