Recombinant Human Tenascin C Protein, CF


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Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human Tenascin C Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to block Fibronectin-mediated adhesion of NIH‑3T3 mouse embryonic fibroblast cells. rhTenascin-C immobilized at 15 μg/mL, in the presence of 0.1 μg/mL human Fibronectin, will block approximately 70%-90% NIH3/T3 cell adhesion (5 x 104 cells/well, 100 μL/well).
Mouse myeloma cell line, NS0-derived human Tenascin C protein
Gly23-Pro625, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
65.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
97 kDa, reducing conditions
Read Publications using
3358-TC in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Tenascin C Protein, CF

  • 150-225
  • Cytotactin
  • Glioma-associated-extracellular matrix antigen
  • GMEM
  • GP 150-225
  • hexabrachion (tenascin C, cytotactin)
  • hexabrachion (tenascin)
  • Hexabrachion
  • HXB
  • HXBcytotactin
  • JI
  • MGC167029
  • Myotendinous antigen
  • neuronectin
  • Tenascin C
  • Tenascin J1
  • tenascin
  • tenascin-C isoform 14/AD1/16
  • Tenascin-C
  • TNC
  • TN-C
  • TNGP


Tenascin C, also known as hexabrachion, cytotactin, neuronectin, GMEM, JI, myotendinous antigen, glioma-associated-extracellular matrix antigen, and GP 150-225, is a member of the Tenascin family of extracellular matrix proteins. It is secreted as a disulfide-linked homohexamer whose subunits can vary in size from approximately 200 kDa to over 300 kDa due to differences in glycosylation (1). Rotary-shadowed electron micrographs of the purified molecule show six strands joined to one another at one end in a globular domain with each arm terminating in a knob-like structure (2-3). The human Tenascin C monomer is synthesized as a precursor with a 22 amino acid (aa) signal sequence and a 2179 aa mature chain (SwissProt # P24821). The mature chain consists of a coiled-coil region (aa 118-145), followed by 15 EGF-like domains, 15 fibronectin type-III domains, and a fibrinogen C-terminal domain. In addition, there are 23 potential sites of N-linked glycosylation. Alternative splicing within the fibronectin type-III repeats produces six isoforms for human Tenascin C. Mature human Tenascin C (isoform 1) shares 84% aa sequence identity with mature mouse Tenascin C. In the developing embryo, Tenascin C is expressed during neural, skeletal, and vascular morphogenesis (1, 2). In the adult, it virtually disappears with continued basal expression detectable only in tendon-associated tissues (1, 2). However, greatup-regulation in expression occurs in tissues undergoing remodeling processes seen during wound repair and neovascularization or in pathological states such as inflammation or tumorigenesis (1, 4-5). Biologically, Tenascin C functions as an adhesion-modulatory extracellular matrix protein (1, 4-8). Specifically, it antagonizes the adhesive effects of fibronectin, and impacts the ability of fibroblasts to deposit and contract the matrix by affecting the morphology and signaling pathways of adherent cells (5-7). Tenascin C acts by blocking syndecan-4 binding at the edges of the wound and by suppressing fibronectin-mediated activation of RhoA and focal adhesion kinase (FAK) (4-8). Tenascin C thus promotes epidermal cell migration and proliferation during wound repair.

  1. Hsia, H.C. and J.E. Schwarzbauer (2005) J. Biol. Chem. 280:26641.
  2. Nies, D.E. et al. (1991) J. Biol. Chem. 266:2818.
  3. Erickson, H.P and J.L. Iglesias (1984) Nature 311:267.
  4. Orend, G. et al. (2003) Oncogene 22:3917.
  5. Wenk, M.B. et al. (2000) J. Cell Biol. 150:913.
  6. Midwood, K.S. et al. (2004) Mol. Biol. Cell 15:5670.
  7. Midwood, K.S. and J. E. Schwarzbauer (2002) Mol. Biol. Cell 13:3601.
  8. Hsia, H.C. and J.E. Schwarzbauer (2006) J. Surg. Res. 136:92.

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Publications for Tenascin C (3358-TC)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Bioassay.

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Showing Publications 1 - 8 of 8.
Publications using 3358-TC Applications Species
AG Hawkins, CM Julian, S Konzen, S Treichel, ER Lawlor, KM Bailey Microenvironmental Factors Drive Tenascin C and Src Cooperation to Promote Invadopodia Formation in Ewing Sarcoma Neoplasia, 2019;21(10):1063-1072. 2019 [PMID: 31521948] (Bioassay, Human) Bioassay Human
JV Lee, CT Berry, K Kim, P Sen, T Kim, A Carrer, S Trefely, S Zhao, S Fernandez, LE Barney, AD Schwartz, SR Peyton, NW Snyder, SL Berger, BD Freedman, KE Wellen Acetyl-CoA promotes glioblastoma cell adhesion and migration through Ca2+-NFAT signaling Genes Dev., 2018;32(7):497-511. 2018 [PMID: 29674394] (Bioassay, Human) Bioassay Human
S Becerril, A Rodríguez, V Catalán, L Méndez-Gim, B Ramírez, N Sáinz, M Llorente, X Unamuno, J Gómez-Ambr, G Frühbeck Targeted disruption of the iNOS gene improves adipose tissue inflammation and fibrosis in leptin-deficient ob/ob mice: role of tenascin C Int J Obes (Lond), 2018;0(0):. 2018 [PMID: 29449623] (Bioassay, Mouse) Bioassay Mouse
S Sarkar, R Mirzaei, FJ Zemp, W Wu, DL Senger, SM Robbins, VW Yong Activation of NOTCH signaling by tenascin-C promotes growth of human brain tumor-initiating cells Cancer Res., 2017;0(0):. 2017 [PMID: 28416488] (Bioassay, Human) Bioassay Human
Hye Jin Park Integrins functioning in uterine endometrial stromal and epithelial cells in estrus Reproduction, 2016;0(0):. 2016 [PMID: 27998942] (Bioassay) Bioassay
Migration of breast cancer cell lines in response to pulmonary laminin 332 Cancer Med, 2016;0(0):. 2016 [PMID: 27878981] (Bioassay, Human) Bioassay Human
Harada M, Kamimura D, Arima Y, Kohsaka H, Nakatsuji Y, Nishida M, Atsumi T, Meng J, Bando H, Singh R, Sabharwal L, Jiang J, Kumai N, Miyasaka N, Sakoda S, Yamauchi-Takihara K, Ogura H, Hirano T, Murakami M Temporal expression of growth factors triggered by epiregulin regulates inflammation development. J Immunol, 2015;194(3):1039-46. 2015 [PMID: 25556244] (Bioassay, Mouse) Bioassay Mouse
Nakamura-Ishizu A, Okuno Y, Omatsu Y, Okabe K, Morimoto J, Uede T, Nagasawa T, Suda T, Kubota Y Extracellular matrix protein tenascin-C is required in the bone marrow microenvironment primed for hematopoietic regeneration. Blood, 2012;119(23):5429-37. 2012 [PMID: 22553313] (Bioassay, Mouse) Bioassay Mouse

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FAQs for Tenascin C (3358-TC). (Showing 1 - 1 of 1 FAQs).

  1. One of our clients is looking for antibodies against tenascin C and fibronectin that could be used for FFPE rat tissues. These antibodies should exclusively recognize tenascin C and fibronectin and do not cross react with each other. Can you inform me if you have these antibodies?
    • Here is a link to Tenascin C antibodies we have validated in rat tissues for IHC-P Here are antibodies we have validated in fibronectin in rat tissues for IHC-P

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Gene Symbol TNC