Recombinant Human SLITRK1 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human SLITRK1 Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly enhance neurite outgrowth when immobilized as a 3 µL droplet containing 100 ng on a nitrocellulose-coated microplate.
• Source: Mouse myeloma cell line, NS0-derived human SLITRK1 protein
  Ala16-Ser616, with a C-terminal 6-His tag
• Accession #: Q96PX8
• N-terminal Sequence: Ala16
• Protein/Peptide Type: Recombinant Proteins
• Gene: SLITRK1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 100-115 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human SLITRK1 Protein, CF

• FLJ54428
• KIAA1910KIAA0918
• leucine rich repeat containing 12
• Leucine-rich repeat-containing protein 12
• LRRC12
• RP11-395N17.1
• SLIT and NTRK-like family, member 1
• SLIT and NTRK-like protein 1
• slit and trk like gene 1
• SLITRK1
• TTM

Background

SLITRK1 (Slit and Trk-like family member 1) is a charter member of the SLITRK family of proteins (1, 2). This family includes six members, all of which contain a slit-like extracellular region, and all of which (save for SLITRK1) contain a Trk-like cytoplasmic region (3). Human SLITRK1 is a type I transmembrane protein 696 amino acids (aa) in length. It contains a 17 aa signal sequence, a 605 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 53 aa cytoplasmic region. The ECD is characterized by the presence of two leucine-rich domains (LRD) that resemble those found in slit. Each LRD (aa 57 - 200; aa 374 - 517) contains six leucine-rich repeats (LRRs), followed by a Cys-rich, C-terminal LRR (aa 212 - 262; aa 529 - 579). The exact function of the LRRs is not known. Based on other LRRs, it might be assumed that they mediate protein-protein-interaction (4). There are multiple, single aa mutations in the ECD. Their significance is unclear (5). One truncating frameshift mutation is known that shows a unique 27 aa substitution after Arg 421 (6). This 448 aa form is associated with Tourette’s Syndrome. As noted, the cytoplasmic region is unique among SLITRK family members. It contains no known signaling motifs, which is in contrast to SLITRK2 - 6 which show TrkA-like tyrosine phosphorylation sites. This difference is reflected in their function. SLITRK1 is a protein that is found in adult neurons of the cerebrum, thalamus and hippocampus. It induces neurite outgrowth whereas SLITRK2 - 6 block neurite extension (3). Human SLITRK1 shares 99%, 98% and 99% aa sequence identity with canine, mouse and bovine SLITRK1, respectively.

1. Aruga, J. et al. (2003) Gene 315:87.
2. Nagase, T. et al. (2001) DNA Res. 8:179.
3. Aruga, J. and K. Mikoshiba (2003) Mol. Cell. Neurosci. 24:117.
4. Enkhbayar, P. et al. (2003) Proteins 54:394.
5. Zuchner, S. et al. (2006) Mol. Psychiat. 11:888.
6. Abelson, J.F. et al. (2005) Science 310:317.

Publications for SLITRK1 (3009-SK)(1)

Publication using 3009-SK

• Stillman AA, Krsnik Z, Sun J, Rasin MR, State MW, Sestan N, Louvi A Developmentally regulated and evolutionarily conserved expression of SLITRK1 in brain circuits implicated in Tourette syndrome. J. Comp. Neurol., 2009-03-01;513(1):21-37. 2009-03-01 [PMID: 19105198] (Western Blot)

Bioinformatics

• Gene Symbol: SLITRK1
• Uniprot:
  • Q96PX8()