Recombinant Human SIRP gamma/CD172g His Avi-tag Protein, CF

Biotinylated Recombinant Human SIRP gamma /CD172g His-tag Avi-tag Protein (Catalog # AVI9999) binds Recombinant Human CD47 Fc Chimera (4670-CD) with an ED50 of 0.150-3.00 μg/mL.

2 μg/lane of Biotinylated Recombinant Human SIRP gamma /CD172g His-tag Avi-tag Protein (Catalog # AVI9999) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 42-49 kDa, under reducing conditions.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human SIRP gamma/CD172g His Avi-tag Protein, CF Summary

• Additional Information: Biotinylated His-tag
• Details of Functionality: Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human SIRP gamma /CD172g His-tag Avi-tag (Catalog # AVI9999) binds Recombinant Human CD47 Fc Chimera (Catalog # 4670-CD) with an ED₅₀ of 0.150-3.00 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human SIRP gamma/CD172g protein
  

  Human SIRPG (Glu29-Pro360) Accession # Q9P1W8.3	6-His tag	Avi-tag
  N-terminus		C-terminus

• Accession #: Q9P1W8.3
• N-terminal Sequence: Glu29
• Structure / Form: Biotinylated via Avi-tag
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 42-49 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human SIRP gamma/CD172g His Avi-tag Protein, CF

• bA77C3.1
• CD172g antigen
• CD172g
• FLJ42230
• signal-regulatory protein beta 2
• Signal-regulatory protein beta-2
• signal-regulatory protein gamma
• SIRP beta 2
• SIRP gamma
• SIRP-B2
• SIRPB2CD172 antigen-like family member B
• SIRP-beta-2
• SIRPG
• SIRPgamma
• SIRP-gamma

Background

Signal regulatory protein gamma (SIRP gamma, designated CD172g), also called SIRP beta 2, is a monomeric 45-47 kDa type I transmembrane protein belonging to the SIRP/SHPS (CD172) family of the Ig superfamily (1-5). SIRP members are "paired receptors" with homology in the extracellular domain but variability in the C‑terminus and signaling function (1, 2). The 387 amino acid (aa) SIRP gamma sequence contains a 28 aa potential signal sequence, a 332 aa extracellular domain (ECD) with four potential N‑glycosylation sites, a 23 aa transmembrane domain and a 4 aa cytoplasmic sequence. SIRP gamma contains one V-type Ig‑like domain that contains a J‑like sequence and two C1-type Ig‑like domains within its ECD (1, 2). Isoforms that lack one (isoform 2, 276 aa) or two (isoform 3, 170 aa) membrane-proximal C‑type Ig-like domains have been described (5). Within the ECD, human SIRP gamma isoform 1 shares 78% aa identity with human SIRP beta 1, and appears to have structurally similar orthologs only in rhesus monkey and chimpanzee (100% and 91% aa identity, respectively) (2). SIRP gamma is the only SIRP known to be expressed on T cells, CD56bright NK cells and activated NK cells; it is not expressed on myeloid cells (5, 6). It shows adhesion to CD47, but at lower affinity than SIRP alpha (6). Expression of SIRP gamma on T cells suggests a role as an accessory protein interacting with CD47‑expressing antigen presenting cells (5-7). Unlike SIRP alpha that has cytoplasmic ITIM domains, and SIRP beta 1 that interacts with DAP-12, SIRP gamma does not contain any obvious signaling motif (1, 2, 6). However, SIRP gamma -mediated adhesion appears to promote antigen-specific T cell proliferation and costimulate T cell activation (5). Engagement of CD47 by SIRP gamma was shown to induce apoptosis on T-cell and monocyte cell lines (6). Our Avi-tag Biotinylated Recombinant Human SIRP gamma /CD172g His-tag features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

1. Barclay, A.N. & M.H. Brown (2006) Nat. Rev. Immunol. 6:457.
2. vanBeek, E.M. et al. (2005) J. Immunol. 175:7781.
3. van den Berg, T.K. et al. (2005) J. Immunol. 175:7788.
4. Ichigotani, Y. et al. (2000) J. Hum. Genet. 45:378.
5. Piccio, L. et al. (2005) Blood 105:2421.
6. Brooke, G. et al. (2004) J. Immunol. 173:2562.
7. Smith, M.J. et al. (2022) Diabetes. 71:350.

Bioinformatics

• Uniprot:
  • Q9P1W8.3()