Recombinant Human Siglec-10 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human Siglec-10 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to bind biotinylated 6’-Sialyllactose-Polyacrylamide in functional ELISA. Whitney, G. et al. (2001) FEBS 268:6083. rhSiglec-10/Fc Chimera immobilized at 5 µg/mL (100 µL/well) on a goat anti-human IgG Fc antibody (Catalog # G-102-C) coated plate can bind biotinylated 6’-Sialyllactose-Polyacrylamide with a linear range of 0.04-2.5 µg/mL.
• Source: Mouse myeloma cell line, NS0-derived human Siglec-10 protein
  

  Human Siglec-10 (Met17-Thr546) Accession # Q96LC7	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q96LC7
• N-terminal Sequence: Met17
• Structure / Form: Disulfide-linked heterodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: SIGLEC10
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 85 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 105-110 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Siglec-10 Fc Chimera Protein, CF

• MGC126774
• PRO940
• sialic acid binding Ig-like lectin 10
• sialic acid-binding Ig-like lectin 10
• Siglec10
• Siglec-10
• siglec-like gene 2
• Siglec-like protein 2
• SLG2
• SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7

Background

Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system (1-3). Siglec-G is the apparent ortholog of human Siglec-10 (4). The human Siglec-10 cDNA encodes a 697 amino acid (aa) precursor that includes a 16 aa signal sequence, a 534 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 126 aa cytoplasmic domain. The ECD contains one Ig-like V-type domain and four Ig-like C2-type domains, while the cytoplasmic domain contains two immunoreceptor tyrosine-based inhibitory motifs (ITIM) (5-8). Five splice variants of human Siglec-10 differ in their deletions within the ECD. A potentially secreted sixth variant contains the Ig-like V-type domain followed by a 45 aa substitution (5-7, 9). Within the ECD, human Siglec-10 is most closely related to Siglec-5 (42% aa sequence identity). It shares 63% aa sequence identity with mouse Siglec-G. Siglec-10 is expressed on eosinophils, neutrophils, monocytes, and B cells (5, 8) with some splice variants predominating in particular cell types and tissue locations (6, 7, 9). It is upregulated on eosinophils in mouse models of allergic respiratory inflammation (10). Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides (7, 11). This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell.(7) When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals (5, 9).

1. Crocker, P.R. (2005) Curr. Opin. Pharmacol. 5:431.
2. Crocker, P.R. (2002) Curr. Opin. Struct. Biol. 12:609.
3. Crocker, P.R. and J. Zhang (2002) Biochem. Soc. Symp. 69:83.
4. Angata, T. et al. (2001) J. Biol. Chem. 276:45128.
5. Whitney, G. et al. (2001) Eur. J. Biochem. 268:6083.
6. Yousef, G.M. et al. (2001) Biochem. Biophys. Res. Commun. 284:900.
7. Li, N. et al. (2001) J. Biol. Chem. 276:28106.
8. Munday, J. et al. (2001) Biochem. J. 355:489.
9. Kitzig, F. et al. (2002) Biochem. Biophys. Res. Commun. 296:355.
10. Aizawa, H. et al. (2003) Genomics 82:521.
11. Rapoport, E. et al. (2003) Bioorg. Med. Chem. Lett. 13:675.

Publications for Siglec-10 (2130-SL)(5)

Publications using 2130-SL

• Liu, T;Wu, G;Gudd, CLC;Trovato, FM;Barbera, T;Liu, Y;Triantafyllou, E;McPhail, MJW;Thursz, MR;Khamri, W; Cis-interaction between CD52 and T cell receptor complex interferes with CD4+ T cell activation in acute decompensation of cirrhosis EBioMedicine 2024-09-13 [PMID: 39276679] (Bioassay, Human)
• Zhang, W;Zeng, Y;Xiao, Q;Wu, Y;Liu, J;Wang, H;Luo, Y;Zhan, J;Liao, N;Cai, Y; An in-situ peptide-antibody self-assembly to block CD47 and CD24 signaling enhances macrophage-mediated phagocytosis and anti-tumor immune responses Nature communications 2024-07-06 [PMID: 38971872] (Flow Cytometry, Human)
• J Terävä, L Tiainen, U Lamminmäki, PL Kellokumpu, K Pettersson, K Gidwani Lectin nanoparticle assays for detecting breast cancer-associated glycovariants of cancer antigen 15-3 (CA15-3) in human plasma PLoS ONE, 2019-07-25;14(7):e0219480. 2019-07-25 [PMID: 31344060] (Bioassay, Human)
• Chen , Guo-Yun, Brown , Nicholas, Wu , Wei, Khedri , Zahra, Yu , Hai, Chen , Xi, van de Vlekkert , Diantha, D'Azzo , Alessand, Zheng , Pan, Liu , Yang Broad and direct interaction between TLR and Siglec families of pattern recognition receptors and its regulation by Neu1. Elife, 2014-09-03;3(0):e04066. 2014-09-03 [PMID: 25187624] (Bioassay, Human)
• Chen GY, Chen X, King S Amelioration of sepsis by inhibiting sialidase-mediated disruption of the CD24-SiglecG interaction. Nat. Biotechnol., 2011-04-10;29(5):428-35. 2011-04-10 [PMID: 21478876] (Binding Assay, Immunoprecipitation, Human, Mouse)

Bioinformatics

• Gene Symbol: SIGLEC10
• Uniprot:
  • Q96LC7()