Recombinant Human Siglec-10 Fc Chimera Avi-tag Protein, CF

When Recombinant Human CD52 Fc Chimera Protein (9116-CD) is immobilized at 5 μg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human Siglec‑10 Fc Chimera Avi-tag (Catalog # AVI2130) that produces 50% of the optimal binding response is found to be approximately 0.75-4.50 μg/mL.

2 μg/lane of Recombinant Siglec-10 Fc Chimera Avi-tag (Catalog # AVI2130) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 95-118 kDa and 190-236 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Siglec-10 Fc Chimera Avi-tag Protein, CF Summary

• Additional Information: Biotinylated
• Details of Functionality: The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human CD52 Fc Chimera Protein (Catalog # 9116-CD) is immobilized at 5 μg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human Siglec‑10 Fc Chimera Avi-tag (Catalog # AVI2130) that produces 50% of the optimal binding response is found to be approximately 0.75-4.50 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Siglec-10 protein
  

  Human Siglec-10 (Met17-Thr546) Accession # Q96LC7.3	IEGRMD	Human IgG1 (Pro100-Lys330)	Avi-tag
  N-terminus			C-terminus

• Accession #: Q96LC7.3
• N-terminal Sequence: Met17
• Structure / Form: Disulfide-linked homodimer, biotinylated via Avi-tag
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Bioactivity2
• Theoretical MW: 87 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 95-118 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Siglec-10 Fc Chimera Avi-tag Protein, CF

• MGC126774
• PRO940
• sialic acid binding Ig-like lectin 10
• sialic acid-binding Ig-like lectin 10
• Siglec10
• Siglec-10
• siglec-like gene 2
• Siglec-like protein 2
• SLG2
• SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7

Background

Sialic acid-binding Ig-like lectin 10 (Siglec-10), also known as Siglec-like protein 2, is an inhibitory I-type lectin of immune regulators belonging to the immunoglobulin superfamily. Siglecs can be divided into 2 classes: evolutionarily conserved Siglecs and CD33-related Siglecs (1). Siglec-10 falls within the CD33-related Siglecs class and has the closest sequence homology to Siglec-5 and Siglec-3 (2). The extracellular domain (ECD) of human Siglec-10 consists of one V-set domain that plays a role in specific sialoglycans recognition and four Ig-like C2-type domains, a single transmembrane domain and a cytoplasmic tail containing three immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (3). Within the ECD, mature human Siglec-10 shares 63% amino acid sequence identity with mouse and rat Siglec-10. Siglec-10 is expressed on eosinophils, neutrophils, monocytes, and B cells (4, 6) with some splice variants predominating in particular cell types and tissue locations (5, 2, 7). It is upregulated on eosinophils in mouse models of allergic respiratory inflammation (8). Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides (2, 9). This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell (2). When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals (4, 7). Additionally, Siglec-10 has been shown to interact with soluble CD52 (10). This interaction inhibits the proliferation of activated T cells and the cytotoxic function of autoimmune CD8+ T cells in type 1 diabetes (11). Our Avi-tag Biotinylated Siglec-10 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

1. Varki, A. and Angata, T. (2006) Glycobiology, 16:1R.
2. Li, N. et al. (2001) J. Biol. Chem. 276:28106.
3. Forgione, R. et al. (2020) iScience. 23:101231.
4. Whitney, G. et al. (2001) Eur. J. Biochem. 268:6083.
5. Yousef, G.M. et al. (2001) Biochem. Biophys. Res. Commun. 284:900.
6. Munday, J. et al. (2001) Biochem. J. 355:489.
7. Kitzig, F. et al. (2002) Biochem. Biophys. Res. Commun. 296:355.
8. Aizawa, H. et al. (2003) Genomics 82:521.
9. Rapoport, E. et al. (2003) Bioorg. Med. Chem. Lett. 13:675.
10. Toh, B.-H. et al. (2013) Cell. Mol. Immunol. 10:379.
11. Bandala-Sanchez, E. et al. (2013) Nat. Immunol. 14:741.

Bioinformatics

• Uniprot:
  • Q96LC7.3()