Recombinant Human Semaphorin 3G Fc Chimera Protein, CF

Recombinant Human Semaphorin 3G Fc Chimera inhibits the proliferation of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 100-600 ng/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Semaphorin 3G Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability to inhibit the proliferation of HUVECumbilical vein endothelial cells.
  The ED₅₀ for this effect is 100-600 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Semaphorin 3G protein
  

  Human Semaphorin 3G (Gly23-Thr782)(R557S, R558S, R560S, R561S, R774S, R777S) Accession # Q9NS98.1	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q9NS98.1
• N-terminal Sequence: Gly23
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 111 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 105-125 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Semaphorin 3G Fc Chimera Protein, CF

• (semaphorin) 3G
• FLJ00014
• MGC119473
• sem2
• sema domain, immunoglobulin domain (Ig), short basic domain, secreted
• SEMA3G
• Semaphorin 3G
• Semaphorin sem2
• Semaphorin-3G

Background

Semaphorin 3G (Sema3G), also known as Semaphorin sem2, is a class 3 member of the semaphorin family initially identified as regulators of neuron-axonal guidance (1, 2). More than 30 semaphorins have been discovered and are divided into eight classes: classes 1 and 4–7 are membrane-associated, while classes 2, 3, and 8 are in a secreted form (1, 2). Mature human Sema3G consists of an N-terminal sema domain, a plexin-semaphorin-integrin (PSI) domain, an Ig-like C2-type domain and a C-terminal basic domain (3). Within the extracellular domain, human Sema3G shares 87% amino acid sequence identity with mouse and rat Sema3G. In vitro analysis shows Sema 3G binds to Neuropilin-2, which forms a receptor complex with Plexin-D1. Via this pathways Sema 3G controls lymphatic vascular patterning by the means of cellular collapse (4). It shows protective effects in ischemic retinopathies by coordinating interactions of beta -catenin and VE-Cadherin in endothelium (5). Sema 3G has been shown to be involved in adipocyte differentiation. Knockdown of Sema3G inhibited weight gain through PI3K/Akt/GSK3 beta signaling in the adipose tissue and the AMPK/SREBP-1c pathway in the liver. Thus, Sema 3G is an adipokine essential for adipogenesis, lipogenesis, and insulin resistance and is associated with obesity (6).

1. Alto, L.T. and Terman, J.R. (2017) Methods Mol. Biol. 1493:1.
2. Jackson, R.E. and Eickholt, B.J. (2009) Curr. Biol. 19:R504.
3. Toledano S. et al. (2019) J. Mol. Sci. 20:556.
4. Liu, X. et al. (2016) Cell Rep. 17:2299.
5. Chen, D. et al. (2021) J. Clin. Invest. 131:e135296.
6. Liu, M. et al. (2020) J. Endocrinol. 244:223.

Bioinformatics

• Uniprot:
  • Q9NS98.1()