Recombinant Human Semaphorin 3C Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Semaphorin 3C Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells to Fibronectin. Banu, N. et al. (2006) FASEB J. 20:2150. Recombinant Human (rh) Semaphorin 3C immobilized at 10 μg/mL, with 2 ng/mL rhFN-1 (Catalog # 4305-FNB), 100 μL/well, will induce approximately 50-75% adhesion.
• Source: Mouse myeloma cell line, NS0-derived human Semaphorin 3C protein
  

  Human Semaphorin 3C (Gly21-Ser738: Arg551Ala, Arg552Ala, Arg611Ala, Arg612Ala) Accession # Q99985	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q99985
• N-terminal Sequence: Gly21
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: SEMA3C
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 107.7 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 110-120 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Semaphorin 3C Fc Chimera Protein, CF

• (semaphorin) 3C
• sema domain, immunoglobulin domain (Ig), short basic domain, secreted
• Sema E
• SEMA3C
• SEMAE
• Semaphorin 3C
• Semaphorin E
• semaphorin-3C
• semaphorin-E
• SEME

Background

Semaphorin 3C (Sema3C; previously SemaE) is one of six Class 3 secreted semaphorins which share 40 - 50% amino acid (aa) identity among themselves. Class 3 semaphorins are potent chemorepellents that function in axon and/or vascular guidance during development, and may be upregulated in tumor progression (1, 2). The 751 aa human Sema3C is highly modular. It contains a 20 aa signal sequence, an ~500 aa N-terminal Sema domain that forms a beta -propeller structure similar to that found in integrin molecules, a cysteine knot, a furin-type cleavage site, an Ig-like domain, and a C-terminal basic domain (1 - 3). Covalent dimerization plus cleavage at the C-terminus are required for activity of class 3 semaphorins (4). Human Sema3C shares at least 95% aa identity with mouse, rat, bovine and canine Sema3C, and 89% and 75% aa identity with chick and zebrafish Sema3C, respectively. Type 3 semaphorins transduce signals through transmembrane plexins, either directly, or by binding associated neuropilin receptors (1, 2). Sema3C signaling is transduced by Plexin-D1 indirectly via Neuropilin 1 or Neuropilin 2 (5). Sema3C is expressed in all somitic motor neurons, in lung buds, and in cardiac neural crest cells during development (1, 5 - 8). Sema3C activates integrins in certain cells, so in addition to its repulsive activities, it sometimes acts as a chemoattractant (6, 9). In the developing nervous system, this chemoattraction appears to complement Sema3A repulsion in adjacent cell layers (1, 6, 7). Sema3C also provides an attractive force opposing Sema6A and Sema6B to guide migration of neural crest endothelial cells to the cardiac outflow tract (10). Consequently, defects in aortic arch formation occur when Sema3C or Plexin D1 genes or Sema3C neuropilin interactions are disrupted (5, 11, 12).

1. Hinck, L. (2004) Dev. Cell 7:783.
2. Neufeld, G. et al. (2005) Front. Biosci. 10:751.
3. Gherardi, E. et al. (2004) Curr. Opin. Struct. Biol. 14:669.
4. Adams, R. H. et al. (1997) EMBO J. 16:6077.
5. Gitler, A. D. et al. (2004) Dev. Cell 7:107.
6. Bagnard, D. et al. (1998) Development 125:5043.
7. Cohen, S. et al. (2005) Eur. J. Neurosci. 21:1767.
8. Puschel, A. W. et al. (1995) Neuron 14:941.
9. Herman, J. G. and G. G. Meadows (2007) Int. J. Oncol. 30:1231.
10. Toyofuku, T. et al. (2008) Dev. Biol. 321:251.
11. Feiner, L. et al. (2001) Development 128:3061.
12. Gu, C. et al. (2003) Dev. Cell 5:45.

Publications for Semaphorin 3C (5570-S3)(4)

Publications using 5570-S3

• Farah, EN;Hu, RK;Kern, C;Zhang, Q;Lu, TY;Ma, Q;Tran, S;Zhang, B;Carlin, D;Monell, A;Blair, AP;Wang, Z;Eschbach, J;Li, B;Destici, E;Ren, B;Evans, SM;Chen, S;Zhu, Q;Chi, NC; Spatially organized cellular communities form the developing human heart Nature 2024-03-13 [PMID: 38480880] (Bioassay, Human)
• Luis F. Martins, Ilaria Brambilla, Alessia Motta, Stefano de Pretis, Ganesh Parameshwar Bhat, Aurora Badaloni et al. Motor neurons use push-pull signals to direct vascular remodeling critical for their connectivity Neuron 2022-12-21 [PMID: 36240771] (Bioassay, Mouse)
• Y Do, JG Cho, JY Park, S Oh, D Park, KH Yoo, MS Lee, BS Kwon, J Kim, Y Yang MiR-146a Regulates Migration and Invasion by Targeting NRP2 in Circulating-Tumor Cell Mimicking Suspension Cells Genes, 2020-12-30;12(1):. 2020-12-30 [PMID: 33396906] (Bioassay, Human)
• A Sema3C Mutant Resistant to Cleavage by Furin (FR-Sema3C) Inhibits Choroidal Neovascularization PLoS ONE, 2016-12-30;11(12):e0168122. 2016-12-30 [PMID: 28036336] (Bioassay, Human)

Bioinformatics

• Gene Symbol: SEMA3C
• Uniprot:
  • Q99985(Human)