Recombinant Human SCGF-alpha/CLEC11a Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human SCGF-alpha/CLEC11a Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Mouse myeloma cell line, NS0-derived human SCGF/CLEC11a protein
  Gly19-Phe323, with an N-terminal Met and a C-terminal 6-His tag
• Accession #: Q9Y240
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: CLEC11A
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 34.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 50 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human SCGF-alpha/CLEC11a Protein, CF

• CLEC11a
• CLECSF3
• LSLCL
• P47
• SCGF

Background

Stem Cell Growth Factor (SCGF), also known as LSLCL, P47, and CLEC11A, is a secreted glycoprotein that acts on primitive hematopoietic stem/progenitor cells to support their proliferation and differentiation. Mature human SCGF contains an N‑terminal Glu‑rich region with an RGD motif, a leucine zipper region, and a C‑terminal lectin‑like domain (1, 2). The RGD motif is absent in mouse and rat SCGF (1). Full length human SCGF (the alpha isoform) shares approximately 83% amino acid (aa) sequence identity with mouse and rat SCGF. A shorter SCGF beta isoform lacks 78 aa of the lectin domain (1‑3). This recombinant protein corresponds to the SCGF alpha isoform. SCGF is produced by various hematopoietic cell lines and by CD34⁺ and CD34‑CD33⁺ bone marrow cells as a 47 kDa sulfated and O‑glycosylated sialoprotein (2‑6). It is also expressed by infiltrating macrophages in some gastrointestinal tract tumors (7). Mouse SCGF mRNA has been detected in proliferating chondrocytes, the primary ossification center, perichondrium, and periosteum (6). SCGF should not be confused with the similarly named SCF (stem cell factor)/c‑Kit Ligand which exerts distinct activities during hematopoietic differentiation. SCGF exhibits erythroid burst (BFU‑E) promoting activity in the absence of SCF/c‑kit Ligand but suppresses the formation of erythroid bursts induced by SCF (4, 6). SCGF cooperates with IL‑3, Flt‑3 Ligand, GM‑CSF, or G‑CSF to enhance the growth of granulocyte‑macrophage colonies (CFU‑GM) (4, 6). It also promotes (with Flt‑3 Ligand and VEGF) the maintenance and expansion of CD34⁺CD133⁺ progenitor cells as well as their differentiation into the endothelial lineage (8, 9). Serum levels of SCGF increase during stem cell transplantation and correlate with the recovery of hematopoiesis (5). Circulating SCGF levels are decreased in severe malarial anemia, and an SCGF promoter polymorphism (-539C/T) is associated with resistance to malarial anemia (10, 11).

1. Mio, H. et al. (1998) Biochem. Biophys. Res. Commun. 249:124.
2. Bannwarth, S. et al. (1998) J. Biol. Chem. 273:1911.
3. Hiraoka, A. et al. (1997) Proc. Natl. Acad. Sci. 94:7577.
4. Hiraoka, A. et al. (1987) Cancer Res. 47:5025.
5. Ito, C. et al. (2003) Bone Marrow Transplant. 32:391.
6. Hiraoka, A. et al. (2001) Hematol. J. 2:307.
7. Da Riva, L. et al. (2011) J. Transl. Med. 9:158.
8. Gehling, U.M. et al. (2000) Blood 95:3106.
9. Loges, S. et al. (2004) Stem Cells Dev. 13:229.
10. Keller, C.C. et al. (2009) Infect. Immun. 77:3864.
11. Ouma, C. et al. (2010) Infect. Immun. 78:453.

Bioinformatics

• Gene Symbol: CLEC11A
• Uniprot:
  • Q9Y240()