Recombinant Human R-Spondin 2 Protein, CF


1 µg/lane of Recombinant Human R‑Spondin 2 was resolved with SDS-PAGE and visualized by silver staining under reducing (R) conditions, showing a single band at 28 kDa.
Recombinant R-Spondin 2 (Catalog # 3266‑RS/CF) induces activation of beta -catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is 0.7-2.8 ng/mL in more

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Recombinant Human R-Spondin 2 Protein, CF Summary

Details of Functionality
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 0.7-2.8 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a.
Mouse myeloma cell line, NS0-derived human R-Spondin 2 protein
Gln22-Gly205, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
No results obtained: Gln22 predicted
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
25-29 kDa, reducing conditions
Read Publications using
3266-RS/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human R-Spondin 2 Protein, CF

  • Cristin 2
  • hRspo2
  • MGC35555
  • MGC43342
  • Roof plate-specific spondin-2
  • RSPO2
  • R-spondin 2 homolog (Xenopus laevis)
  • RSpondin 2
  • R-Spondin 2
  • R-spondin-2


Roof plate-specific Spondin 2 isoform 1 (R‑Spondin 2, RSPO2), also known as cysteine‑rich and single thrombospondin domain containing protein 2 (Cristin 2), is a 33 kDa secreted protein that belongs to the R-Spondin family (1‑3). The four R‑Spondins regulate Wnt/ beta -catenin signaling and overlap in expression and function (1‑3). Like other R‑Spondins, RSPO2 contains two adjacent cysteine‑rich furin-like domains (aa 90‑134) followed by a thrombospondin (TSP-1) motif (aa 144‑204) and a C-terminal region rich in basic residues (aa 207‑243). The basic region binds heparin and mediates cell surface retention and extracellular matrix attachment while the furin‑like domains are required for Wnt/ beta -catenin signaling (1, 3, 4). RSPO2 contains one potential N‑glycosylation site. Mature human RSPO2 shares 97‑98% aa identity with mouse, rat, equine, canine and bovine RSPO2 and ~40% aa identity with RSPO1, RSPO3 and RSPO4. Of the three reported splice isoforms of human R‑Spondin 2, isoform 2 lacks residues 1 ‑ 67 of isoform 1, while isoform 3 has a glycine substitution for residues 32‑95 of isoform 1 (5). Human RSPO2 is expressed in organs of endodermal origin in adults, including intestine and lung, and is down‑regulated in tumors of these tissues (1). In the embryonic mouse, RSPO2 expression is concentrated in the apical epidermal ridge, hippocampus, and developing muscle, teeth and bones (1, 6). Deletion of RSPO2 results in down‑regulation of Wnt activity in these areas, malformations of the facial skeleton and limbs, and respiratory failure at birth (7‑9). RSPO2 is an extracellular potentiator of Wnt/ beta -catenin signaling (3, 4). It functions at least in part by binding LRP‑6, stimulating its long-term phosphorylation and down‑regulating its internalization (3, 4). RSPO proteins, especially RSPO2 and RSPO3, also antagonize DKK1 activity by interfering with DKK1‑mediated LRP‑6 and Kremen association (10).

  1. Kazanskaya, O. et al. (2004) Dev. Cell 7:525.
  2. Kim, K.-A. et al. (2006) Cell Cycle 5:23.
  3. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
  4. Li, S-J. et al. (2009) Cell Signal. 21:916.
  5. Swiss-Prot Accession # Q6UXX9.
  6. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
  7. Yamada, W. et al. (2009) Biochem. Biophys. Res. Commun. 381:453.
  8. Jin, Y.-R. et al. (2011) Dev. Biol. 352:1.
  9. Nam, J.-S. et al. (2007) Dev. Biol. 311:124.
  10. Kim, K.-A. et al. (2007) Mol. Biol. Cell 19:2588.

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Publications for R-Spondin 2 (3266-RS/CF)(13)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Bioassay, Cell Culture.

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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using 3266-RS/CF Applications Species
M Haas, JL Gómez Vázq, DI Sun, HT Tran, M Brislinger, A Tasca, O Shomroni, K Vleminckx, P Walentek δN-Tp63 Mediates Wnt/ β-Catenin-Induced Inhibition of Differentiation in Basal Stem Cells of Mucociliary Epithelia Cell Rep, 2019;28(13):3338-3352.e6. 2019 [PMID: 31553905] (Bioassay, Human) Bioassay Human
S Park, J Cui, WA Yu, L Wu, K Carmon, QJ Liu Differential activities and mechanisms of the four R-Spondins in potentiating Wnt/?-catenin signaling J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29752411] (Bioassay, Human) Bioassay Human
M Szemes, A Greenhough, Z Melegh, S Malik, A Yuksel, D Catchpoole, K Gallacher, M Kollareddy, JH Park, K Malik Wnt Signalling Drives Context-Dependent Differentiation or Proliferation in Neuroblastoma Neoplasia, 2018;20(4):335-350. 2018 [PMID: 29505958] (Bioassay, Human) Bioassay Human
AM Lebensohn, R Rohatgi R-spondins can potentiate WNT signaling without LGRs Elife, 2018;7(0):. 2018 [PMID: 29405118] (Bioassay, Human) Bioassay Human
YE Kang, JM Kim, KS Kim, JY Chang, M Jung, J Lee, S Yi, HW Kim, JT Kim, K Lee, MJ Choi, SK Kang, SE Lee, HS Yi, BS Koo, M Shong Upregulation of RSPO2-GPR48/LGR4 signaling in papillary thyroid carcinoma contributes to tumor progression Oncotarget, 2017;8(70):114980-114994. 2017 [PMID: 29383135] (Bioassay, Human) Bioassay Human
Aberrantly expressed LGR4 empowers Wnt signaling in multiple myeloma by hijacking osteoblast-derived R-spondins Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):. 2016 [PMID: 28028233] (Bioassay, Human) Bioassay Human
Hiroaki Nakashima R-spondin 2 promotes acetylcholine receptor clustering at the neuromuscular junction via Lgr5 Sci Rep, 2016;6(0):28512. 2016 [PMID: 27328992] (Bioassay, Mouse) Bioassay Mouse
Chartier C, Raval J, Axelrod F, Bond C, Cain J, Dee-Hoskins C, Ma S, Fischer M, Shah J, Wei J, Ji M, Lam A, Stroud M, Yen W, Yeung P, Cancilla B, O'Young G, Wang M, Kapoun A, Lewicki J, Hoey T, Gurney A Therapeutic Targeting of Tumor-Derived R-Spondin Attenuates beta-Catenin Signaling and Tumorigenesis in Multiple Cancer Types. Cancer Res, 2016;76(3):713-23. 2016 [PMID: 26719531] (Bioassay, Mouse) Bioassay Mouse
Wu C, Qiu S, Lu L, Zou J, Li W, Wang O, Zhao H, Wang H, Tang J, Chen L, Xu T, Sun Z, Liao W, Luo G, Lu X RSPO2-LGR5 signaling has tumour-suppressive activity in colorectal cancer. Nat Commun, 2014;5(0):3149. 2014 [PMID: 24476626]
Xie, Yang, Zamponi, Raffaell, Charlat, Olga, Ramones, Melissa, Swalley, Susanne, Jiang, Xiaomo, Rivera, Daniel, Tschantz, William, Lu, Bo, Quinn, Lisa, Dimitri, Chris, Parker, Jefferso, Jeffery, Doug, Wilcox, Sheri K, Watrobka, Mike, LeMotte, Peter, Granda, Brian, Porter, Jeffrey, Myer, Vic E, Loew, Andreas, Cong, Feng Interaction with both ZNRF3 and LGR4 is required for the signalling activity of R-spondin. EMBO Rep, 2013;14(12):1120-6. 2013 [PMID: 24165923] (Cell Culture, Human) Cell Culture Human
Show All 13 Publications.

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Gene Symbol RSPO2