Recombinant Human PSP94/MSMB Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Human PSP94/MSMB Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized rhPSP94 at 1 µg/mL (100 µL/well) can bind rhCRISP-3 with a linear range of 0.03‑10 µg/mL.
Source
E. coli-derived human PSP94/MSMB protein
Ser21-Ile114, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Ser21
Protein/Peptide Type
Recombinant Proteins
Gene
MSMB
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
11.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
3780-PS in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in HCl.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human PSP94/MSMB Protein, CF

  • IGBF
  • MSMB
  • MSPB
  • PIP
  • PN44
  • PRPS
  • PSP57
  • PSP94

Background

PSP94 (prostate secretory protein of 94 amino acids; also named  beta -MSP) is a secreted, non-glycosylated member of the beta -microseminoprotein family (1). The 94 amino acid (aa) mature PSP94 contains no classic motifs or domains, but does have ten Cys that are conserved across species (1, 2). It is expressed in mucoid secretions, but its function is unknown (2, 3). PSP94 is abundant in prostatic fluid, which is the exclusive source in rodents (4). Gastric and respiratory secretory epithelia are also significant sources in humans (2, 3). Human PSP94 circulates bound to a 71 kDa PSP binding protein, possibly disulfide-linked (5). The seminal fluid protein CRISP-3 can also bind PSP94 (6). PSP94 has been proposed as an alternative to PSA as a serum marker for prostate cancer. When total (bound plus free) PSP94 is considered, its secretion is found to be down-regulated in cancer cells, creating below normal circulating levels (7, 8). Its size is predicted at 11 kDa, but may appear to be 16 kDa due to anomalous migration (3). A 61 aa variant, formed by C-terminal proteolysis, is increased in prostate secretions from patients with benign prostatic hyperplasia (9). A prostate-specific alternate splice form shows a substitution of 41 aa for the C-terminal 78 aa (10). Mature human PSP94 shares 53%, 46%, 43% and 42% aa identity with porcine, rat, mouse and chicken PSP94, respectively. Most of the ten primate sequences available show less than 80% aa identity. PSP94 has been proposed as a species barrier protein due to its low evolutionary conservation and abundance in seminal fluid (11).

  1. Seidah, N.G. et al. (1984) FEBS Lett. 175:349.
  2. Ulvsback, M. et al. (1989) Biochem. Biophys. Res. Comm. 164:1310.
  3. Weiber, H. et al. (1990) Am. J. Pathol. 137:593.
  4. Thota, A. et al. (2003) J. Cell. Biochem. 88:999.
  5. Wu, D. et al. (1999) J. Cell. Biochem. 76:71.
  6. Udby, L. et al. (2005) Biochem. Biophys. Res. Comm. 333:555.
  7. Reeves, J.R. et al. (2005) Biochem. J. 385:105.
  8. Nam, R.K. et al. (2006) J. Urol. 175:1291.
  9. Xu, K. et al. (2003) Electrophoresis 24:1311.
  10. Xuan, J.W. et al. (1995) Oncogene 11:1041.
  11. Xuan, J.W. et al. (1999) DNA Cell Biol. 18:11.

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3780-PS
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Publications for PSP94/MSMB (3780-PS)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol MSMB
Uniprot