Recombinant Human PSG1 Protein, CF

When Recombinant Human SLITRK5 (Catalog # 2587-SK) is coated at 500 ng/mL,Recombinant Human PSG1(Catalog # 9334-P1)binds withan ED50 = 50-300ng/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human PSG1 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human SLITRK5 (Catalog # 2587-SK) is coated at 500 ng/mL, Recombinant Human PSG1 (Catalog # 9334-P1) binds with an ED₅₀ = 50-300 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human PSG1 protein
  Gln35-Pro419, with a C-terminal 6-His tag
• Accession #: P11464
• N-terminal Sequence: Gln35 & Val36
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 64-77 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 2 weeks, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human PSG1 Protein, CF

• B1G1
• B1G1CD66 antigen-like family member F
• CD66f antigen
• CD66f
• DHFRP2
• Fetal liver non-specific cross-reactive antigen 1/2
• FLJ90598
• FLJ90654
• FL-NCA-1/2
• PBG1
• pregnancy specific beta-1-glycoprotein 1
• pregnancy-specific B-1 glycoprotein
• Pregnancy-specific beta-1 glycoprotein C/D
• pregnancy-specific beta-1-glycoprotein 1
• Pregnancy-specific glycoprotein 1
• PS-beta-C/D
• PS-beta-G-1
• PSbG1
• PSBG-1
• PSBG1SP1
• PSG1
• PSG-1
• PSG95
• PSGGA
• PSGGAPSG95
• PSGIIA

Background

Pregnancy-specific beta-1-glycoprotein 1 (PSG1), also called SP1, PSG1 or B1G1 and designated CD66f, is a secreted glycoprotein of the human PSG family within the CEA (carcinoembryonic antigen) superfamily (1-3). PSG1 shares 84-91% amino acid (aa) sequence identity with the most similar family members, PSG3, 4, 6, 7, and 8. Mature PSG1 contains a V-type Ig-like domain that is important for adhesion (N1), and three C2-type Ig-like domains (A1, A2 and B2). Three potential isoforms vary at the extreme C-terminus. Although rodents express pregnancy-specific glycoproteins, identity with human PSG proteins is low and limited to the N1 domain (3). PSG1 is one of the most abundant fetal proteins found in maternal blood during pregnancy. It is secreted by syncytiotrophoblasts and exerts proangiogenic effect by inducing VEGF-A secretion and formation of tubes in endothelial cells (4). PSG1 also serves an immunomodulatory function through
up-regulation of TGF-beta in macrophages, monocytes, and trophoblasts (5). In addition PSG1 has been shown to induce secretion of anti-inflammatory cytokines
IL-10 and IL-6 in human monocytes (6). PSG1 binds Integrin alpha IIb beta 3 and inhibits platelet-fibrinogen interactions (7). Recent studies also suggest PSG1's involvement in cancer biology and correlation with chemotherapy resistance in breast cancer (8).

1. Watanabe, S. and J.Y. Chou (1988) J. Biol. Chem. 263:2049.
2. Leslie, K.K. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5822.
3. McLellan, A.S. et al. (2005) BMC Evol. Biol. 5:39.
4. Lisboa, F. et al. (2011) J Biol Chem. 286:7577.
5. Ha, C. et al. (2010) Biol Rep. 83:27.
6. Snyder, S. et al. (2001) Am J Reprod Immunol. 45:205.
7. Shanley D. et al. (2013) PLoS One. 8:e57491.
8. He, D. et al. (2016) Clin Sci (Lond). 130:2267.

Bioinformatics

• Uniprot:
  • P11464()