Recombinant Human PSG1 Protein, CF

Images

 
When Recombinant Human SLITRK5 (Catalog # 2587-SK) is coated at 500 ng/mL,Recombinant Human PSG1(Catalog # 9334-P1)binds withan ED50 = 50-300ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human PSG1 Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human SLITRK5 (Catalog # 2587-SK) is coated at 500 ng/mL, Recombinant Human PSG1 (Catalog # 9334-P1) binds with an ED50 = 50-300 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human PSG1 protein
Gln35-Pro419, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Gln35 & Val36
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
64-77 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 2 weeks, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human PSG1 Protein, CF

  • B1G1
  • B1G1CD66 antigen-like family member F
  • CD66f antigen
  • CD66f
  • DHFRP2
  • Fetal liver non-specific cross-reactive antigen 1/2
  • FLJ90598
  • FLJ90654
  • FL-NCA-1/2
  • PBG1
  • pregnancy specific beta-1-glycoprotein 1
  • pregnancy-specific B-1 glycoprotein
  • Pregnancy-specific beta-1 glycoprotein C/D
  • pregnancy-specific beta-1-glycoprotein 1
  • Pregnancy-specific glycoprotein 1
  • PS-beta-C/D
  • PS-beta-G-1
  • PSbG1
  • PSBG-1
  • PSBG1SP1
  • PSG1
  • PSG-1
  • PSG95
  • PSGGA
  • PSGGAPSG95
  • PSGIIA

Background

Pregnancy-specific beta-1-glycoprotein 1 (PSG1), also called SP1, PSG1 or B1G1 and designated CD66f, is a secreted glycoprotein of the human PSG family within the CEA (carcinoembryonic antigen) superfamily (1-3). PSG1 shares 84-91% amino acid (aa) sequence identity with the most similar family members, PSG3, 4, 6, 7, and 8. Mature PSG1 contains a V-type Ig-like domain that is important for adhesion (N1), and three C2-type Ig-like domains (A1, A2 and B2). Three potential isoforms vary at the extreme C-terminus. Although rodents express pregnancy-specific glycoproteins, identity with human PSG proteins is low and limited to the N1 domain (3). PSG1 is one of the most abundant fetal proteins found in maternal blood during pregnancy. It is secreted by syncytiotrophoblasts and exerts proangiogenic effect by inducing VEGF-A secretion and formation of tubes in endothelial cells (4). PSG1 also serves an immunomodulatory function through
up-regulation of TGF-beta in macrophages, monocytes, and trophoblasts (5). In addition PSG1 has been shown to induce secretion of anti-inflammatory cytokines
IL-10 and IL-6 in human monocytes (6). PSG1 binds Integrin alpha IIb beta 3 and inhibits platelet-fibrinogen interactions (7). Recent studies also suggest PSG1's involvement in cancer biology and correlation with chemotherapy resistance in breast cancer (8).
  1. Watanabe, S. and J.Y. Chou (1988) J. Biol. Chem. 263:2049.
  2. Leslie, K.K. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5822.
  3. McLellan, A.S. et al. (2005) BMC Evol. Biol. 5:39.
  4. Lisboa, F. et al. (2011) J Biol Chem. 286:7577.
  5. Ha, C. et al. (2010) Biol Rep. 83:27.
  6. Snyder, S. et al. (2001) Am J Reprod Immunol. 45:205.
  7. Shanley D. et al. (2013) PLoS One. 8:e57491.
  8. He, D. et al. (2016) Clin Sci (Lond). 130:2267.

Customers Who Viewed This Item Also Viewed...

AF4256
Species: Hu
Applications: WB
NBP2-67471
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-29463
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB110-75039
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
AF2818
Species: Hu
Applications: WB, ICC
NB100-56583
Species: Hu, Rb
Applications: WB, Flow, IHC-P
NB100-56530
Species: Hu
Applications: WB
NBP2-67861
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-89544
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
NBP1-86614
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-55298
Species: Hu
Applications: WB, IHC, IHC-P
NB100-74359
Species: Hu, Mu, Ch
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-46085
Species: Hu
Applications: WB, IHC
9334-P1
Species: Hu
Applications: Bioactivity

Publications for PSG1 (9334-P1) (0)

There are no publications for PSG1 (9334-P1).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PSG1 (9334-P1) (0)

There are no reviews for PSG1 (9334-P1). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for PSG1 (9334-P1) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

PSG1 Antibody
NBP1-58001

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human PSG1 Protein, CF and receive a gift card or discount.

Bioinformatics

Uniprot