Recombinant Human PGLYRP3/PGRP-I alpha Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human PGLYRP3/PGRP-I alpha Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When peptidoglycan is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Human PGLYRP3/PGRP-I alpha that produces 50% optimal binding response is 10-60 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human PGLYRP3/PGRP-I alpha protein
  Trp18-His341, with a C-terminal 6-His tag
• Accession #: Q96LB9
• N-terminal Sequence: Trp18
• Structure / Form: Disulfide-linked Homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: PGLYRP3
• Purity: >90%, by SDS-PAGE with silver staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 34-40 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE with silver staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human PGLYRP3/PGRP-I alpha Protein, CF

• peptidoglycan recognition protein 3
• peptidoglycan recognition protein I alpha
• Peptidoglycan recognition protein I-alpha
• Peptidoglycan recognition protein intermediate alpha
• peptidoglycan recognition protein-I-alpha
• PGLYRP3
• PGLYRPIalphaMGC149197
• PGRP1A
• PGRPI alpha
• PGRP-I alpha
• PGRPIA
• PGRP-Ialpha
• PGRPIAPGRP-I-alpha

Background

PGLYRP3, also known as PGRP-1 alpha , is a member of the peptidoglycan recognition protein family of innate immunity proteins (1, 2). Human PGLYRP3 is expressed in the skin, eyes, salivary glands, throat, tongue, esophagus, stomach, and intestine (3). Mature human PGLYRP3 contains two nonidentical PGRP domains, and it shares 74% and 75% amino acid sequence identity with mouse and rat PGLYRP3, respectively (1, 4). It is secreted as disulfide-linked homodimers and binds peptidoglycan (PGN) and PGN-containing Gram-positive bacteria (1, 3). PGLYRP3 is directly cytotoxic against pathogenic and nonpathogenic Gram-positive and Gram-negative bacteria (3, 5). Its bactericidal activity requires physiological concentrations of Zn²⁺ (5). PGLYRP3 has also been shown to have an anti-inflammatory role in intestinal epithelial cells (6). PGLYRP3 knockout mice are more sensitive to the development of experimental dermatitis and DSS-induced colitis than wild type mice (7-9). In humans, PGLYRP3 single nucleotide polymorphisms have been associated with inflammatory bowel disease (10).

1. Liu, C. et al. (2001) J. Biol. Chem. 276:34686.
2. Park, S.Y. et al. (2011) PLoS One 6:e24961.
3. Lu, X. et al. (2006) J. Biol. Chem. 281:5895.
4. Royet, J. and R. Dziarski (2007) Nat. Rev. Microbiol. 5:264.
5. Wang, M. et al. (2007) J. Immunol. 178:3116.
6. Zenhom, M. et al. (2012) Immunobiology 217:412.
7. Park, S.Y. et al. (2011) PLoS One 6:e24961.
8. Saha, S. et al. (2010) Cell Host Microbe 8:147.
9. Jing, X. et al. (2014) J. Immunol. 193:3055.
10. Zulfiqar, F. et al. (2013) PLoS One 8:e67393.

Bioinformatics

• Gene Symbol: PGLYRP3
• Uniprot:
  • Q96LB9()