Recombinant Human PGLYRP1/PGRP-S Protein, CF

When peptidoglycan is coated at 1 µg/mL, 100 µL/well, Recombinant Human PGLYRP1/PGRP-S binds with an ED50 of 0.75-4.5 ng/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human PGLYRP1/PGRP-S Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When peptidoglycan is coated at 1 µg/mL (100 µL/well), the concentration of Recombinant Human PGLYRP1/PGRP-S that produces 50% optimal binding response is 0.75-4.5 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human PGLYRP1/PGRP-S protein
  Gln22-Pro196, with a C-terminal 6-His tag
• Accession #: O75594
• N-terminal Sequence: No results obtained. Gln22 predicted
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: PGLYRP1
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 23-27 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human PGLYRP1/PGRP-S Protein, CF

• peptidoglycan recognition protein 1
• Peptidoglycan recognition protein short
• PGLYRP
• PGLYRP1
• PGRPpeptidoglycan recognition protein
• PGRPS
• PGRP-S
• PGRP-SMGC126894
• PGRPSMGC126896
• TAG7
• TNF superfamily, member 3 (LTB)-like (peptidoglycan recognition protein)
• TNFSF3L

Background

The human PGRP family is comprised of four peptidoglycan recognition proteins that may function as innate immunity pattern recognition molecules (1, 2). Termed PGRP-L, PGRP-I alpha , PGRP-I beta and PGRP-S, they are all products of separate genes, and all are named for the relative length of their translated product (3). PGRP-L (for long) is 576 amino acids (aa) in length, while PGRP-I alpha and I beta are (I) intermediate in length at 341 aa and 373 aa, respectively, and PGRP-S is the shortest at 196 aa in length (3, 4). All human PGRPs bind peptidoglycan and Gram-positive bacteria, and all have at least three C-terminal PGRP domains at variable sites that are highly conserved from insects to mammals (3). Human PGRP-S, the first described member of the family, is a 28 kDa secreted glycoprotein associated with neutrophils (4). The mature molecule is 175 aa in length and contains three variably-sized peptide-carbohydrate recognition sequences of 15 aa, 29 aa and 49 aa, respectively. Human PGRP-S is 72%, 71% and 70% aa identical to mouse, bovine and rat mature PGRP-S, respectively. Studies with PGRP-S deficient mice indicate that knock-out mice have increased susceptibility to infections with non-pathogenic bacteria. Neutrophils from knock-out mice exhibit normal phagocytosis of bacteria but are defective in intracellular killing and digestion of nonpathogenic bacteria (5). The longer three PGRP members are all membrane-bound molecules that contain two membrane-spanning segments. Both the N- and C-termini are depicted as being extracellular with a joining cytoplasmic domain. All three transmembrane forms show at least one PGRP domain on the C-terminal extracellular region; other PGRP domains are variably distributed over their two extracellular and one cytoplasmic region (3).

1. Girardin, S.E. and D.J. Philpott (2004) Eur. J. Immunol. 34:1777.
2. Steiner, H. (2004) Immunol. Rev. 198:83.
3. Liu, C. et al. (2001) J. Biol. Chem. 276:34686.
4. Kang, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:10078.
5. Dziarski, R. et al. (2003) Blood 102:689.

Publications for PGLYRP1/PGRP-S (2590-PGB)(5)

Publications using 2590-PGB

• Chen, S;Putnik, R;Li, X;Diwaker, A;Vasconcelos, M;Liu, S;Gondi, S;Zhou, J;Guo, L;Xu, L;Temme, S;Bersch, K;Hyland, S;Yin, J;Burstein, E;Bahnson, BJ;Gildersleeve, JC;Grimes, CL;Reinecker, HC; PGLYRP1-mediated intracellular peptidoglycan detection promotes intestinal mucosal protection Nature communications 2025-02-21 [PMID: 39984444] (Bioassay, Mouse)
• Reinecker, HC;Chen, S;Putnik, R;Li, X;Diwaker, A;Vasconcelos, M;Liu, S;Zhou, J;Guo, L;Xu, L;Temme, JS;Bersch, K;Hyland, S;Yin, J;Burstein, E;Gildersleeve, J;Grimes, C; PGLYRP-1 mediated intracellular peptidoglycan detection promotes mucosal protection Research square 2024-10-14 [PMID: 39483916] (Immunoassay Development, N/A)
• Giraud, J;Chalopin, D;Ramel, E;Boyer, T;Zouine, A;Derieppe, MA;Larmonier, N;Adotevi, O;Le Bail, B;Blanc, JF;Laurent, C;Chiche, L;Derive, M;Nikolski, M;Saleh, M; THBS1+ myeloid cells expand in SLD hepatocellular carcinoma and contribute to immunosuppression and unfavorable prognosis through TREM1 Cell reports 2024-02-12 [PMID: 38350444] (Bioassay, Human)
• A Gupta, G Arora, CE Rosen, Z Kloos, Y Cao, J Cerny, A Sajid, D Hoornstra, M Golovchenk, N Rudenko, U Munderloh, JW Hovius, CJ Booth, C Jacobs-Wag, NW Palm, AM Ring, E Fikrig A human secretome library screen reveals a role for Peptidoglycan Recognition Protein 1 in Lyme borreliosis PLoS Pathog, 2020-11-11;16(11):e1009030. 2020-11-11 [PMID: 33175909] (ICC, Borrelia burgdorferi (Lyme Disease))
• Read C, Kuijper J, Hjorth S, Heipel M, Tang X, Fleetwood A, Dantzler J, Grell S, Kastrup J, Wang C, Brandt C, Hansen A, Wagtmann N, Xu W, Stennicke V Cutting Edge: identification of neutrophil PGLYRP1 as a ligand for TREM-1. J Immunol, 2015-01-16;194(4):1417-21. 2015-01-16 [PMID: 25595774] (Surface Plasmon Resonance, Human)

Bioinformatics

• Gene Symbol: PGLYRP1
• Uniprot:
  • O75594()