Recombinant Human PANP Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human PANP Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of Neuro‑2A mouse neuroblastoma cells. The ED₅₀ for this effect is 25-100 ng/mL.
• Source: Human embryonic kidney cell, HEK293-derived human PANP protein
  

  Human PANP (Ser76-Pro178) Accession # Q8IYJ0	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q8IYJ0
• N-terminal Sequence: Ser76 & Val80
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: PIANP
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 37 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 58-69 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human PANP Fc Chimera Protein, CF

• C12orf53
• chromosome 12 open reading frame 53
• PANP
• PIANP
• PILR alpha associated neural protein

Background

PILR‑alpha associated neural protein (PANP) is an approximately 30 kDa type 1 transmembrane glycoprotein that contains a 147 amino acid (aa) extracellular domain (ECD) and an 83 aa cytoplasmic domain (1). Within the ECD, human PANP shares 93% aa sequence identity with mouse and rat PANP. Alternative splicing may generate an additional isoform with a 6 aa truncation at the C‑terminus. PANP is preferentially expressed in the central nervous system (1). It binds to PILR‑alpha, an inhibitory receptor that is expressed on dendritic cells, neutrophils, and macrophages (1, 2). PILR‑alpha additionally binds to CD99, NPDC‑1, CL‑P1/COLEC12, and glycoprotein B of Herpes simplex virus type 1 (3‑5). PILR‑alpha binding requires the presence of sialylated O‑linked carbohydrates on each of these ligands, including PANP (1, 3, 4, 6). The in cis association of PILR‑alpha with sialylated molecules on the neutrophil surface inhibits beta integrin activation and neutrophil extravasation during inflammatory responses (7).

1. Kogure, A. et al. (2011) Biochem. Biophys. Res. Commun. 405:428.
2. Fournier, N. et al. (2000) J. Immunol. 165:1197.
3. Wang, J. et al. (2008) J. Immunol. 180:1686.
4. Sun, Y. et al. (2012) J. Biol. Chem. 287:15837.
5. Satoh, T. et al. (2008) Cell 132:935.
6. Wang, J. et al. (2009) J. Virol. 83:13042.
7. Wang, J. et al. (2013) Nat. Immunol. 14:34.

Bioinformatics

• Gene Symbol: PIANP
• Uniprot:
  • Q8IYJ0()