CL-P1/COLEC12 Antibody

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HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human CL‑P1/COLEC12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2690, filled histogram) or isotype control antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

CL-P1/COLEC12 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human CL‑P1/COLEC12 isoform 1
Leu57-Leu742
Accession # Q5KU26
Specificity
Detects CL‑P1/COLEC12 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross‑reactivity with recombinant mouse CL-P1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
COLEC12
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF2690 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CL-P1/COLEC12 Antibody

  • CL-P1
  • CLP1hCL-P1
  • COLEC12
  • collectin placenta 1
  • Collectin placenta protein 1
  • collectin sub-family member 12
  • collectin-12
  • NSR2
  • Nurse cell scavenger receptor 2
  • SCARA4
  • SCARA4NSR2
  • Scavenger receptor class A member 4
  • scavenger receptor class A, member 4
  • SRCL Type I
  • SRCLScavenger receptor with C-type lectin

Background

Collectins are a family of Ca++-dependent, C-type lectins that contain a collagenous domain and function as recognition molecules for molecular patterns found on pathogens (1‑4). Human collectin placenta 1 (CL-P1; also known as collectin sub-family member 12 and SRCL type I [scavenger receptor with C-type lectin type I]) is a 110 kDa member of the collectin family of glycoproteins (5, 6). CL-P1 is the only collectin known to be membrane bound, while CL-L1 (collectin liver-1) is the only known cytoplasmic collectin (1). Human CL-P1 is synthesized as a 742 amino acid (aa) type II transmembrane glycoprotein that contains an N-terminal 39 aa cytoplasmic domain, a 17 aa transmembrane segment, and a 686 aa C-terminal extracellular region (6). The short cytoplasmic domain contains an internalization motif (Y-K-R-F) while the extracellular region is complex, demonstrating a coiled-coil segment, a Ser-Thr rich region, a collagen-like structure and a C-type lectin/ carbohydrate recognition domain (CRD). Notably, this CRD recognizes galactose (and fucose) within the context of asialo‑orosomucoids associated with the Lewisx epitope (7, 8). CL-P1 has a 300 kDa trimeric form due to its collagen-like and coiled-coil helical domains (1, 5). There is a 97 kDa, alternate splice form of CL-P1 (SRCL type II) that shows a 120 aa truncation at the C-terminus. This effectively removes the entire CRD found on full‑length CL-P1 (6). Human CL-P1 is 93% aa identical to mouse CL-P1 over the entire extracellular region, and 87% aa identical over the CRD region (5, 9). Human CL-P1 is known to be expressed in vascular endothelial cells (5).

  1. van de Wetering, JK. et al. (2004) Eur. J. Biochem. 271:1229.
  2. Holmskov, U. et al. (2003) Annu. Rev. Immunol. 21:547.
  3. Hoppe, H-J. and K. Reid (1994) Protein Sci. 3:1143.
  4. Hickling, T.P. et al. (2004) J. Leukoc. Biol. 75:27.
  5. Ohtani, K. et al. (2001) J. Biol. Chem. 276:44222.
  6. Nakamura,K. et al. (2001) Biochem. Biophys. Res. Commun. 280:1028.
  7. Coombs, P.J. et al. (2005) J. Biol. Chem. 280:22993.
  8. Yoshida, T. et al. (2003) J. Biochem. 133:271.
  9. Nakamura, K. et al. (2001) Biochim. Biophys. Acta 1522:53.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CL-P1/COLEC12 Antibody (AF2690)(4)

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 3 applications: Flow Cytometry, IHC-Fr, Western Blot.


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Flow Cytometry
(1)
IHC-Fr
(1)
Western Blot
(2)
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Human
(3)
N/A
(1)
All Species

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