Recombinant Human OCAM/NCAM2 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human OCAM/NCAM2 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of T47D human breast cancer cells. The ED₅₀ for this effect is typically 0.3-1.5 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human OCAM/NCAM2 protein
  

  Human OCAM/NCAM2 Leu20-Asn697 (Arg49Glu, Gly72Glu, Leu163Phe, Gly374Asp) Accession # AAB80803	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: AAB80803
• N-terminal Sequence: Leu20
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: NCAM2
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 102.6 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 100-140 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human OCAM/NCAM2 Fc Chimera Protein, CF

• NCAM2
• N-CAM-2
• NCAM-2
• NCAM21MGC51008
• neural cell adhesion molecule 2 precursor10
• neural cell adhesion molecule 2
• OCAM
• Rb-8
• RNCAM

Background

OCAM (olfactory cell adhesion molecule), also known as NCAM2 (neural cell adhesion molecule 2) and RNCAM (Rb‑8 neural cell adhesion molecule) is an approximately 100 kDa type I transmembrane glycoprotein belonging to the NCAM family within the immunoglobulin superfamily (1‑5). OCAM dimerizes and participates in homophilic adhesion, but unlike NCAM1/CD56, it is not polysialic acid‑glycosylated, and does not participate in heparin sulfate binding or heterophilic adhesion (2). The 837 amino acid (aa) form of human or mouse OCAM contains a 19 aa signal sequence, a 678 aa extracellular domain (ECD) with five Ig‑like C2‑type domains and two fibronectin type III domains, a transmembrane domain and a 119 aa cytoplasmic domain (2‑4). The first two Ig‑like domains mediate homodimer formation in trans (6). In mouse, a glycosyl phosphatidylinositol‑linked form may show differential expression and function on olfactory axons (1‑3, 7). The ECD of human OCAM shares 93%, 92% and 95% aa sequence identity with mouse, rat, and bovine OCAM, respectively. Human OCAM also shares approximately 44% aa sequence identity with human NCAM1. OCAM is expressed by a subset of axons in the olfactory, vomeronasal, and retrosplenial cortex in a zone‑specific manner (1‑3, 7‑9). It is thought to be important for organization of axons and dendrites and segregation of axodendritic and dendrodendritic synapses within glomeruli (1, 7, 10, 11). Because OCAM is encoded on human chromosome 21, it is implicated in neurological abnormalities in Down Syndrome (trisomy 21) (1, 3, 4). It may also be implicated in development of autism and Alzheimer’s disease, and over‑expressed in some breast and prostate cancers (1, 5).

1. Winther, M. et al. (2012) Int. J. Biochem. Cell Biol. 44:441.
2. Yoshihara, Y. et al. (1997) J. Neurosci. 17:5930.
3. Alenius, M. and S. Bohm (1997) J. Biol. Chem. 272:26083.
4. Paoloni-Giacobino, A. et al. (1997) Genomics 43:43.
5. Takahashi, S. et al. (2011) Cancer Sci. 102:808.
6. Kulahin, N. et al. (2011) Structure 19:203.
7. Alenius, M. and S. Bohm (2003) Development 130:917.
8. von Campenhausen, H. et al. (1997) Neuroreport 8:2607.
9. Ichinohe, N. et al. (2003) Eur. J. Neurosci. 18:1764.
10. Walz, A. et al. (2006) Mol. Cell. Neurosci. 32:1.
11. Borisovska, M. et al. (2011) J. Physiol. 589:1927.

Publications for OCAM/NCAM2 (7729-CM)(1)

Publication using 7729-CM

• Deleyrolle L, Sabourin J, Rothhut B, Fujita H, Guichet P, Teigell M, Ripoll C, Chauvet N, Perrin F, Mamaeva D, Noda T, Mori K, Yoshihara Y, Hugnot J OCAM Regulates Embryonic Spinal Cord Stem Cell Proliferation by Modulating ErbB2 Receptor. PLoS ONE, 2015-04-13;10(4):e0122337. 2015-04-13 [PMID: 25875008] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: NCAM2
• Uniprot:
  • AAB80803()