Recombinant Human NRG1-beta 1/HRG1-beta 1 ECD Protein

Images

 
1 μg/lane of Recombinant Human NRG1-beta 1/HRG1-beta 1 Extracellular Domain (Catalog # 377-HB) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 36 kDa.
Recombinant Human NRG1-beta 1/HRG1-beta 1 Extracellular Domain (Catalog # 377-HB) stimulates cell proliferation of the MCF-7 human breast cancer cell line in a serum free assay. The ED50 for this effect is 2.5-12.5 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human NRG1-beta 1/HRG1-beta 1 ECD Protein Summary

Details of Functionality
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 2.5-12.5 ng/mL.
Source
E. coli-derived human Neuregulin-1 beta 1/NRG1 beta 1 protein
Ser2-Lys246
Accession #
N-terminal Sequence
Ser2
Protein/Peptide Type
Recombinant Proteins
Gene
NRG1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
26.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
377-HB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NRG1-beta 1/HRG1-beta 1 ECD Protein

  • ARIA
  • GGF
  • GGF2
  • HGL
  • HRG
  • HRG1
  • HRGA
  • MST131
  • MSTP131
  • NDF
  • neuregulin 1
  • Neuregulin1 beta 1
  • Neuregulin-1 beta 1
  • NRG1-IT2
  • SMDF

Background

The Neuregulin family of structurally related glycoproteins comprises products from four distinct but related genes, Nrg-1, Nrg-2, Nrg-3, and Nrg-4. Through alternative splicing or the use of alternative promoters, Nrg-1 has been shown to encode more than 14 soluble or transmembrane proteins. The extracellular domain of the transmembrane NRG1 isoforms can be proteolytically cleaved to release soluble growth factors. All NRG1 isoforms contain an EGF-like domain ( alpha - or beta -splice variant that differ in their C-terminal region) that is required for their direct binding to the ErbB3 or ErbB4 receptor tyrosine kinases. The ErbB3 or ErbB4 subsequently recruits and heterodimerizes with ErbB2, resulting in tyrosine phosphorylation and NRG1 signaling. NRG1 isoforms can be classified into three major subtypes. Type I (Neu Differentiation Factor, NDF; Heregulin, HRG; Acetylcholine Receptor Inducing Activity, ARIA) and type II (Glial Growth Factor, GGF) NRG1s have an immunoglobulin (Ig)-like domain N-terminal to the EGF-like domain. Type I NRG1s differ from type II NRG1s by having a glycosylation-rich domain between the Ig-like and the EGF-like domains. Type III NRG1s (Sensory and Motor neuron-Derived Factor) lacks the Ig-like domain but has a cysteine rich domain (CRD) instead. NRG1 isoforms show distinct spatial and temporal expression patterns. These proteins play important roles during development of both the nervous system and the heart. They have been shown to regulate the selective expression of neurotransmitter receptors in neurons and at the neuromuscular junction, and promote the differentiation and development of Schwann cells from neural crest stem cells. NRG1s have also been shown to be involved in the establishment of the oligodendroglial lineage.

  1. Buonanno, A., and G.D Fischbach (2001) Curr. Opin. Neurobiol. 11:287.
  2. Adlkofer, K. and C. Lai (2000) Glia 29:104.
  3. Garratt, A.N. et al., (2000) BioEssays 22:987.

Customers Who Viewed This Item Also Viewed...

NB120-3104
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IP
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NBP1-85010
Species: Hu
Applications: WB, IHC, IHC-P
MAB3481
Species: Hu
Applications: Flow, CyTOF-ready, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
NBP2-52983
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-00527
Species: Hu, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-33177
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-12553
Species: Hu
NBP1-85299
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NB110-40773
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-85818
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NB100-866
Species: Hu, Mu, Rt, Po
Applications: WB, IHC, IHC-P, PEP-ELISA
377-HB
Species: Hu
Applications: Bioactivity

Publications for Neuregulin-1 beta 1/NRG1 beta 1 (377-HB)(12)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 3 applications: Binding Assay, Bioassay, In Vivo.


Filter By Application
Binding Assay
(1)
Bioassay
(10)
In Vivo
(1)
All Applications
Filter By Species
Human
(7)
Mouse
(1)
Rat
(2)
Canine
(1)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using 377-HB Applications Species
T Osada, ZC Hartman, J Wei, G Lei, AC Hobeika, WR Gwin, MA Diniz, N Spector, TM Clay, W Chen, MA Morse, HK Lyerly Polyfunctional anti-human epidermal growth factor receptor 3 (anti-HER3) antibodies induced by HER3 vaccines have multiple mechanisms of antitumor activity against therapy resistant and triple negative breast cancers Breast Cancer Res., 2018;20(1):90. 2018 [PMID: 30092835] (Bioassay, Human) Bioassay Human
G Scapin, VP Dandey, Z Zhang, W Prosise, A Hruza, T Kelly, T Mayhood, C Strickland, CS Potter, B Carragher Structure of the Insulin Receptor-Insulin Complex by Single Particle CryoEM analysis Nature, 2018;0(0):. 2018 [PMID: 29512653] (Bioassay) Bioassay
Gu J, Yang J, Chang Q, Liu Z, Ghayur T, Gu J Identification of Anti-EGFR and Anti-ErbB3 Dual Variable Domains Immunoglobulin (DVD-Ig) Proteins with Unique Activities. PLoS ONE, 2015;10(5):e0124135. 2015 [PMID: 25997020] (Bioassay, Human) Bioassay Human
Gericota B, Anderson J, Mitchell G, Borjesson D, Sturges B, Nolta J, Sieber-Blum M Canine epidermal neural crest stem cells: characterization and potential as therapy candidate for a large animal model of spinal cord injury. Stem Cells Transl Med, 2014;3(3):334-45. 2014 [PMID: 24443004] (Bioassay, Canine) Bioassay Canine
Gostring L, Malm M, Hoiden-Guthenberg I, Frejd FY, Stahl S, Lofblom J, Gedda L Cellular Effects of HER3-Specific Affibody Molecules. PLoS ONE, 2012;7(6):e40023. 2012 [PMID: 22768204] (Binding Assay, Human) Binding Assay Human
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012;487(7408):505-9. 2012 [PMID: 22763448] (Bioassay, Human) Bioassay Human
Casazza A, Finisguerra V, Capparuccia L Sema3E-Plexin D1 signaling drives human cancer cell invasiveness and metastatic spreading in mice. J. Clin. Invest., 2010;120(8):2684-98. 2010 [PMID: 20664171] (Bioassay, Human) Bioassay Human
Sheng, Qing, Liu, Xinggang, Fleming, Eleanor, Yuan, Karen, Piao, Huiying, Chen, Jinyun, Moustafa, Zeinab, Thomas, Roman K, Greulich, Heidi, Schinzel, Anna, Zaghlul, Sara, Batt, David, Ettenberg, Seth, Meyerson, Matthew, Schoeberl, Birgit, Kung, Andrew L, Hahn, William, Drapkin, Ronny, Livingston, David M, Liu, Joyce F An activated ErbB3/NRG1 autocrine loop supports in vivo proliferation in ovarian cancer cells. Cancer Cell, 2010;17(3):298-310. 2010 [PMID: 20227043] (Bioassay, Human) Bioassay Human
Yamauchi J, Miyamoto Y, Chan JR, Tanoue A ErbB2 directly activates the exchange factor Dock7 to promote Schwann cell migration. J. Cell Biol., 2008;181(2):351-65. 2008 [PMID: 18426980] (Bioassay, Rat) Bioassay Rat
Barkho BZ, Song H, Aimone JB Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation. Stem Cells Dev., 2006;15(3):407-21. 2006 [PMID: 16846377] (Bioassay, Rat) Bioassay Rat
Show All 12 Publications.

Reviews for Neuregulin-1 beta 1/NRG1 beta 1 (377-HB) (0)

There are no reviews for Neuregulin-1 beta 1/NRG1 beta 1 (377-HB). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for Neuregulin-1 beta 1/NRG1 beta 1 (377-HB) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for Neuregulin-1 beta 1/NRG1 beta 1 (377-HB)

Discover related pathways, diseases and genes to Neuregulin-1 beta 1/NRG1 beta 1 (377-HB). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Neuregulin-1 beta 1/NRG1 beta 1 (377-HB)

Discover more about diseases related to Neuregulin-1 beta 1/NRG1 beta 1 (377-HB).
 

Pathways for Neuregulin-1 beta 1/NRG1 beta 1 (377-HB)

View related products by pathway.

PTMs for Neuregulin-1 beta 1/NRG1 beta 1 (377-HB)

Learn more about PTMs related to Neuregulin-1 beta 1/NRG1 beta 1 (377-HB).

Blogs on Neuregulin-1 beta 1/NRG1 beta 1

There are no specific blogs for Neuregulin-1 beta 1/NRG1 beta 1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human NRG1-beta 1/HRG1-beta 1 ECD Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol NRG1
Uniprot