Recombinant Human NELL2 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human NELL2 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.3-1.8 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived human NELL2 protein
  Leu22-Leu816, with a C-terminal 6-His tag
• Accession #: Q99435
• N-terminal Sequence: Leu22
• Protein/Peptide Type: Recombinant Proteins
• Gene: NELL2
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 90 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 100-125 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Sodium Citrate, NaCl and EDTA.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NELL2 Protein, CF

• Mel91
• nel (chicken)-like 2
• NELL2
• NEL-like 2 (chicken)
• NEL-like protein 2
• Nel-related protein 2
• NRP2
• NRP2neural epidermal growth factor-like 2
• protein kinase C-binding protein NELL2

Background

NELL2 is an approximately 140 kDa secreted glycoprotein that exhibits several actions during nervous system development. NELL2 associates into a homotrimer and contains one Laminin G domain, one vWF-C domain, and six EGF-like domains, followed by two more vWF-C domains (1). Mature human NELL2 shares 94% aa sequence identity with mouse and rat NELL2. Alternative splicing generates a cytosolic isoform that associates with PKC beta 1 and can inhibit PKC signaling (2). NELL2 is widely expressed in neurons of the central and peripheral nervous systems, particularly in the hippocampus, hypothalamus, cerebral cortex, and cerebellar cortex (3-5). It is selectively expressed in VGAT1⁺ or VGAT2⁺ glutamatergic neurons (4, 6). During development, NELL2 is expressed in select neural crest cells, retinal ganglion cells, and hypaxial muscle precursor cells of the dermomyotome (7). It promotes the differentiation and migration of both motor and sensory neurons (8), enhances neuronal survival (9), and promotes neuronal aggregation by inducing N-Cadherin up-regulation (10). NELL2 supports the release of GnRH from the hypothalamus which is important for the onset of female puberty (5, 6). NELL2 is additionally expressed in renal tubules but is down-regulated in renal cancer (11).

1. Watanabe, T.K. et al. (1996) Genomics 38:273.
2. Lee, D.Y. et al. (2014) Biochem. Biophys. Res. Commun. 454:459.
3. Oyasu, M. et al. (2000) Brain Res. Mol. Brain Res. 76:151.
4. Jeong, J.K. et al. (2008) Mol. Cells 26:186.
5. Zhou, S.S. and P. Li (2014) Genet. Mol. Res. 13:6672.
6. Ha, C.M. et al. (2008) Neuroendocrinology 88:199.
7. Nelson, B.R. et al. (2002) Mech. Dev. 119S:S11.
8. Nelson, B.R. et al. (2004) Dev. Biol. 270:322.
9. Aihara, K. et al. (2003) Mol. Brain Res. 116:86.
10. Kim, D.H. et al. (2014) PLoS One 9:e85898.
11. Nakamura, R. et al. (2015) Cancer Sci. 106:656.

Publications for NELL2 (8946-NL)(2)

Publications using 8946-NL

• Jones, K;Keddy, C;Jenkins, C;Nicholson, K;Shinde, U;Davare, MA; Novel insight into mechanisms of ROS1 catalytic activation via loss of the extracellular domain Scientific reports 2024-09-27 [PMID: 39333184] (Bioassay, Human)
• P Jayabal, F Zhou, X Lei, X Ma, B Blackman, ST Weintraub, PJ Houghton, Y Shiio NELL2-cdc42 signaling regulates BAF complexes and Ewing sarcoma cell growth Cell Reports, 2021-07-06;36(1):109254. 2021-07-06 [PMID: 34233189] (Bioassay, Human)

Bioinformatics

• Gene Symbol: NELL2
• Uniprot:
  • Q99435(Human)